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• W2891947261 startingPage "e00805" @default.
• W2891947261 abstract "This study aimed to evaluate in vitro antioxidant capacity of olive leaf extract (OLE), Olea europaea L., and its protective effect on peroxyl radical-induced oxidative damage in human erythrocytes.The OLE was evaluated by the following assays: i) total phenolic and flavonoid content; ii) oleuropein content; iii) Ferric reducing antioxidant power (FRAP); iv) antioxidant activity against ABTS•+, DPPH• and reactive oxygen and nitrogen species: superoxide anion ( O2·- ), hypochlorous acid (HOCl) and nitric oxide (NO•) and v) protective effect on peroxyl radical-induced oxidative damages in human erythrocytes as hemolysis, thiobarbituric acid reactive substances (TBARS) formation and oxyhemoglobin oxidation.Total phenolic and flavonoid contents were 131.7 ± 9.4 mg gallic acid equivalents/g dry weight (dw) and 19.4 ± 1.3 mg quercetin equivalents/g dw, respectively. Oleuropein content was 25.5 ± 5.2 mg/g dw. FRAP analysis was 281.8 ± 22.8 mg trolox equivalent/g dw and OLE inhibited ABTS•+ (50% effective concentration (EC50) = 16.1 ± 1.2 μg/mL) and DPPH• (EC50 = 13.8 ± 0.8 μg/mL). The extract demonstrated effective ability to scavenge O2·- (EC50 = 52.6 ± 2.1 μg/mL), NO• (EC50 = 48.4 ± 6.8 μg/mL) and HOCl (EC50 = 714.1 ± 31.4 μg/mL). The extract inhibited peroxyl radical-induced hemolysis (EC50 = 11.5 ± 1.5 μg/mL), TBARS formation (EC50 = 38.0 ± 11.7 μg/mL) and hemoglobin oxidation (EC50 = 186.3 ± 29.7 μg/mL) in erythrocytes.OLE is an important source of natural antioxidants; it has effective antioxidant activity against different reactive species and protects human erythrocytes against oxidative damage." @default.
• W2891947261 created "2018-09-27" @default.
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• W2891947261 date "2018-09-01" @default.
• W2891947261 modified "2023-10-14" @default.
• W2891947261 title "In vitro antioxidant activity of olive leaf extract (Olea europaea L.) and its protective effect on oxidative damage in human erythrocytes" @default.
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• W2891947261 doi "https://doi.org/10.1016/j.heliyon.2018.e00805" @default.
• W2891947261 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6148714" @default.
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