FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891955057> ?p ?o ?g. }

• W2891955057 endingPage "225" @default.
• W2891955057 startingPage "220" @default.
• W2891955057 abstract "PCR enables sensitive and specific detection of infectious disease agents, but application in point-of-care diagnostic testing remains scarce. A compact tool that runs PCR assays in less than a few minutes and that relies on mass-producible, disposable reactors could revolutionize while-you-wait molecular testing. We here exploit well-established semiconductor manufacturing processes to produce silicon ultra-fast quantitative PCR (UF-qPCR) chips that can run PCR protocols with limited assay optimization. A total of 110 clinical samples were analyzed for the detection of group B streptococci using both a validated benchtop and an on-chip qPCR assay. For the on-chip assay, the total reaction time was reduced after optimization to less than 5 min. The standard curve, spanning a concentration range of 5 log units, yielded a PCR efficiency of 94%. The sensitivity obtained was 96% (96/100; CI: 90–98%) and the specificity 70% (7/10; CI: 40–90%). We show that if melting analyses would be integrated, the obtained sensitivity would drop slightly to 93% (CI: 86–96%), while the specificity would increase to 100% (CI: 72% − 100%). In comparison to the benchtop reference qPCR assay performed on a LightCycler©96, the on-chip assay demonstrated a highly significant qualitative (Spearman's rank correlation) and quantitative (linear regression) correlation. Using a mass-producible qPCR chip and limited assay optimization, we were able to develop a validated qPCR protocol that can be carried out in less than five minutes. The analytical performance of the microchip-based UF-qPCR system was shown to match that of a benchtop assay. This is the first report to provide UF-qPCR validation using clinical samples. We demonstrate that qPCR-based while-you-wait testing is feasible without jeopardizing assay performance." @default.
• W2891955057 created "2018-09-27" @default.
• W2891955057 creator A5006339665 @default.
• W2891955057 creator A5006651644 @default.
• W2891955057 creator A5035750721 @default.
• W2891955057 creator A5038809737 @default.
• W2891955057 creator A5050966931 @default.
• W2891955057 creator A5052183888 @default.
• W2891955057 creator A5080323121 @default.
• W2891955057 creator A5082334350 @default.
• W2891955057 date "2019-01-01" @default.
• W2891955057 modified "2023-10-05" @default.
• W2891955057 title "Ultra-fast, sensitive and quantitative on-chip detection of group B streptococci in clinical samples" @default.
• W2891955057 cites W1759005947 @default.
• W2891955057 cites W1967745807 @default.
• W2891955057 cites W1973651571 @default.
• W2891955057 cites W1980863083 @default.
• W2891955057 cites W2002045697 @default.
• W2891955057 cites W2011348920 @default.
• W2891955057 cites W2018379564 @default.
• W2891955057 cites W2022745284 @default.
• W2891955057 cites W2035443290 @default.
• W2891955057 cites W2073745233 @default.
• W2891955057 cites W2074305200 @default.
• W2891955057 cites W2076652076 @default.
• W2891955057 cites W2077823618 @default.
• W2891955057 cites W2081998687 @default.
• W2891955057 cites W2091477342 @default.
• W2891955057 cites W2100531163 @default.
• W2891955057 cites W2103992736 @default.
• W2891955057 cites W2106072567 @default.
• W2891955057 cites W2108729723 @default.
• W2891955057 cites W2112523009 @default.
• W2891955057 cites W2113558688 @default.
• W2891955057 cites W2146168433 @default.
• W2891955057 cites W2159759971 @default.
• W2891955057 cites W2167903799 @default.
• W2891955057 cites W2168420558 @default.
• W2891955057 cites W2607152017 @default.
• W2891955057 cites W2607530362 @default.
• W2891955057 cites W2611953063 @default.
• W2891955057 cites W2749954635 @default.
• W2891955057 cites W2769044360 @default.
• W2891955057 doi "https://doi.org/10.1016/j.talanta.2018.09.041" @default.
• W2891955057 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30348381" @default.
• W2891955057 hasPublicationYear "2019" @default.
• W2891955057 type Work @default.
• W2891955057 sameAs 2891955057 @default.
• W2891955057 citedByCount "12" @default.
• W2891955057 countsByYear W28919550572019 @default.
• W2891955057 countsByYear W28919550572020 @default.
• W2891955057 countsByYear W28919550572021 @default.
• W2891955057 countsByYear W28919550572022 @default.
• W2891955057 countsByYear W28919550572023 @default.
• W2891955057 crossrefType "journal-article" @default.
• W2891955057 hasAuthorship W2891955057A5006339665 @default.
• W2891955057 hasAuthorship W2891955057A5006651644 @default.
• W2891955057 hasAuthorship W2891955057A5035750721 @default.
• W2891955057 hasAuthorship W2891955057A5038809737 @default.
• W2891955057 hasAuthorship W2891955057A5050966931 @default.
• W2891955057 hasAuthorship W2891955057A5052183888 @default.
• W2891955057 hasAuthorship W2891955057A5080323121 @default.
• W2891955057 hasAuthorship W2891955057A5082334350 @default.
• W2891955057 hasConcept C104317684 @default.
• W2891955057 hasConcept C142724271 @default.
• W2891955057 hasConcept C185592680 @default.
• W2891955057 hasConcept C204787440 @default.
• W2891955057 hasConcept C2780367336 @default.
• W2891955057 hasConcept C43617362 @default.
• W2891955057 hasConcept C48023723 @default.
• W2891955057 hasConcept C55493867 @default.
• W2891955057 hasConcept C70784841 @default.
• W2891955057 hasConcept C71924100 @default.
• W2891955057 hasConceptScore W2891955057C104317684 @default.
• W2891955057 hasConceptScore W2891955057C142724271 @default.
• W2891955057 hasConceptScore W2891955057C185592680 @default.
• W2891955057 hasConceptScore W2891955057C204787440 @default.
• W2891955057 hasConceptScore W2891955057C2780367336 @default.
• W2891955057 hasConceptScore W2891955057C43617362 @default.
• W2891955057 hasConceptScore W2891955057C48023723 @default.
• W2891955057 hasConceptScore W2891955057C55493867 @default.
• W2891955057 hasConceptScore W2891955057C70784841 @default.
• W2891955057 hasConceptScore W2891955057C71924100 @default.
• W2891955057 hasLocation W28919550571 @default.
• W2891955057 hasLocation W28919550572 @default.
• W2891955057 hasOpenAccess W2891955057 @default.
• W2891955057 hasPrimaryLocation W28919550571 @default.
• W2891955057 hasRelatedWork W1980297543 @default.
• W2891955057 hasRelatedWork W2359478292 @default.
• W2891955057 hasRelatedWork W2374391544 @default.
• W2891955057 hasRelatedWork W2378851794 @default.
• W2891955057 hasRelatedWork W2387450218 @default.
• W2891955057 hasRelatedWork W2413749996 @default.
• W2891955057 hasRelatedWork W2415719425 @default.
• W2891955057 hasRelatedWork W2543682440 @default.
• W2891955057 hasRelatedWork W2891955057 @default.
• W2891955057 hasRelatedWork W3032558312 @default.
• W2891955057 hasVolume "192" @default.

• next