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• W2891977888 abstract "Understanding the topology adopted by individual G-quadruplex (GQ)-forming sequences in vivo and targeting a specific GQ motif among others in the genome will have a profound impact on GQ-directed therapeutic strategies. However, this remains a major challenge as most of the tools poorly distinguish different GQ conformations and are not suitable for both cell-free and in-cell analysis. Here, we describe an innovative probe design to investigate GQ conformations and recognition in both cell-free and native cellular environments by using a conformation-sensitive dual-app nucleoside analogue probe. The nucleoside probe, derived by conjugating fluorobenzofuran at the 5-position of 2'-deoxyuridine, is composed of a microenvironment-sensitive fluorophore and an in-cell NMR compatible 19F label. This noninvasive nucleoside, incorporated into the human telomeric DNA oligonucleotide repeat, serves as a common probe to distinguish different GQ topologies and quantify topology-specific binding of ligands by fluorescence and NMR techniques. Importantly, unique signatures displayed by the 19F-labeled nucleoside for different GQs enabled a systematic study in Xenopus laevis oocytes to provide new structural insights into the GQ topologies adopted by human telomeric overhang in cells, which so far has remained unclear. Studies using synthetic cell models, immunostaining on fixed cells, and crystallization conditions suggest that parallel GQ is the preferred conformation of telomeric DNA repeat. However, our findings using the dual-app probe clearly indicate that multiple structures including hybrid-type parallel-antiparallel and parallel GQs are formed in the cellular environment. Taken together, our findings open new experimental strategies to investigate topology, recognition, and therapeutic potential of individual GQ-forming motifs in a biologically relevant context." @default.
• W2891977888 created "2018-09-27" @default.
• W2891977888 creator A5040361326 @default.
• W2891977888 creator A5041237847 @default.
• W2891977888 creator A5066827195 @default.
• W2891977888 date "2018-09-07" @default.
• W2891977888 modified "2023-10-13" @default.
• W2891977888 title "A Dual-App Nucleoside Probe Provides Structural Insights into the Human Telomeric Overhang in Live Cells" @default.
• W2891977888 cites W158754959 @default.
• W2891977888 cites W1721320694 @default.
• W2891977888 cites W1765730586 @default.
• W2891977888 cites W1789563956 @default.
• W2891977888 cites W1914649288 @default.
• W2891977888 cites W1965740503 @default.
• W2891977888 cites W1967345725 @default.
• W2891977888 cites W1968000756 @default.
• W2891977888 cites W1969039627 @default.
• W2891977888 cites W1981981820 @default.
• W2891977888 cites W1990635146 @default.
• W2891977888 cites W1998085469 @default.
• W2891977888 cites W2000818448 @default.
• W2891977888 cites W2006934214 @default.
• W2891977888 cites W2007812774 @default.
• W2891977888 cites W2008120871 @default.
• W2891977888 cites W2009581739 @default.
• W2891977888 cites W2012219231 @default.
• W2891977888 cites W2015755605 @default.
• W2891977888 cites W2016491299 @default.
• W2891977888 cites W2020038227 @default.
• W2891977888 cites W2026088563 @default.
• W2891977888 cites W2032339090 @default.
• W2891977888 cites W2035030010 @default.
• W2891977888 cites W2037863064 @default.
• W2891977888 cites W2038138229 @default.
• W2891977888 cites W2043720815 @default.
• W2891977888 cites W2044285379 @default.
• W2891977888 cites W2047873173 @default.
• W2891977888 cites W2048286707 @default.
• W2891977888 cites W2051270516 @default.
• W2891977888 cites W2053146271 @default.
• W2891977888 cites W2054599183 @default.
• W2891977888 cites W2055132246 @default.
• W2891977888 cites W2055493491 @default.
• W2891977888 cites W2056201880 @default.
• W2891977888 cites W2059113504 @default.
• W2891977888 cites W2062724831 @default.
• W2891977888 cites W2075530432 @default.
• W2891977888 cites W2076345534 @default.
• W2891977888 cites W2082613621 @default.
• W2891977888 cites W2088139266 @default.
• W2891977888 cites W2102212065 @default.
• W2891977888 cites W2108763751 @default.
• W2891977888 cites W2111061634 @default.
• W2891977888 cites W2111271543 @default.
• W2891977888 cites W2119684905 @default.
• W2891977888 cites W2122141896 @default.
• W2891977888 cites W2124582915 @default.
• W2891977888 cites W2134166196 @default.
• W2891977888 cites W2140590042 @default.
• W2891977888 cites W2141218040 @default.
• W2891977888 cites W2151510603 @default.
• W2891977888 cites W2155773930 @default.
• W2891977888 cites W2159105945 @default.
• W2891977888 cites W2166965022 @default.
• W2891977888 cites W2168146598 @default.
• W2891977888 cites W2251344425 @default.
• W2891977888 cites W2290989326 @default.
• W2891977888 cites W2316902933 @default.
• W2891977888 cites W2318569979 @default.
• W2891977888 cites W2330574937 @default.
• W2891977888 cites W2426177481 @default.
• W2891977888 cites W2477718940 @default.
• W2891977888 cites W2510312826 @default.
• W2891977888 cites W2524542824 @default.
• W2891977888 cites W2539691326 @default.
• W2891977888 cites W2583080711 @default.
• W2891977888 cites W2586894471 @default.
• W2891977888 cites W2588267243 @default.
• W2891977888 cites W2590327834 @default.
• W2891977888 cites W2603728845 @default.
• W2891977888 cites W2612060709 @default.
• W2891977888 cites W2621182399 @default.
• W2891977888 cites W2734518194 @default.
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• W2891977888 doi "https://doi.org/10.1021/jacs.8b08436" @default.
• W2891977888 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6348103" @default.
• W2891977888 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30192541" @default.
• W2891977888 hasPublicationYear "2018" @default.
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