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• W2891994829 abstract "Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to 'fish' native cytoplasmic or nuclear proteins from single mammalian cells, without compromising cell viability, followed by ex cellulo quantitative detection. Our work paves the way for spatiotemporally-controlled, quantitative, live, single-cell proteomics." @default.
• W2891994829 created "2018-09-27" @default.
• W2891994829 creator A5048984195 @default.
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• W2891994829 date "2018-09-11" @default.
• W2891994829 modified "2023-09-29" @default.
• W2891994829 title "Protein fishing from single live cells" @default.
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• W2891994829 doi "https://doi.org/10.1186/s12951-018-0395-5" @default.
• W2891994829 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6134770" @default.
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