FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2891997162> ?p ?o ?g. }

• W2891997162 abstract "Bone marrow mesenchymal stem cells (BMSCs) are considered the most important seed cells in bone tissue engineering. The present study aimed to investigate the potential of rabbit BMSCs in osteogenesis after the transfection of human BMP-2 and EGFP recombinant adenovirus. Rabbit BMSCs were isolated and the surface stem cell makers, including CD29, CD44 and CD45 were detected by flow cytometry. The expression of BMP-2 mRNA and protein in BMSCs were detected by reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. After an induction with osteogenic medium, the alkaline phosphatase (ALK) activity at 7 days, the type I collagen at 14 days, and the calcium nodules at 21 days were performed using an ALK activity kit, immunohistochemical staining and alizarin red S staining, respectively. The expression levels of proteins related to the Wnt signaling pathway were detected by western blot analysis. The positive rates of CD29, CD44 and CD45 were 62.92±1.99, 93.55±0.99 and 0.21±0.12%. The expression of BMP-2 mRNA and protein was significantly upregulated in Ad-BMP-2/EGFP transfected BMSCs. Furthermore, Ad-BMP-2/EGFP induced ALP activity, promoted the production of type I collagen and calcium nodule formation in rabbit BMSCs. The levels of β-catenin, cyclin D1, Runx2 and c-myc were upregulated in Ad-hBMP-2/EGFP transfected BMSCs, while the level of GSK3β was significantly decreased. Results also indicated that the overexpression of BMP-2 by Ad-hBMP-2/EGFP enhanced the osteogenic differentiation ability of cultured rabbit BMSCs via stimulating the Wnt signaling pathway with the accumulation of β-catenin and suppression of GSK3β. The Ad-hBMP-2/EGFP transfected rabbit BMSCs are expected to be a good seed cell in bone tissue engineering." @default.
• W2891997162 created "2018-09-27" @default.
• W2891997162 creator A5002661071 @default.
• W2891997162 creator A5003285644 @default.
• W2891997162 creator A5023160300 @default.
• W2891997162 creator A5023613911 @default.
• W2891997162 creator A5038099693 @default.
• W2891997162 creator A5050745133 @default.
• W2891997162 creator A5063395984 @default.
• W2891997162 creator A5065116828 @default.
• W2891997162 creator A5073964986 @default.
• W2891997162 date "2018-09-13" @default.
• W2891997162 modified "2023-10-15" @default.
• W2891997162 title "Enhancement of osteogenesis of rabbit bone marrow derived mesenchymal stem cells by transfection of human BMP‑2 and EGFP recombinant adenovirus via Wnt signaling pathway" @default.
• W2891997162 cites W1779098814 @default.
• W2891997162 cites W1997991074 @default.
• W2891997162 cites W2014861948 @default.
• W2891997162 cites W2021306520 @default.
• W2891997162 cites W2024027335 @default.
• W2891997162 cites W2036122797 @default.
• W2891997162 cites W2037810763 @default.
• W2891997162 cites W2062811107 @default.
• W2891997162 cites W2071642802 @default.
• W2891997162 cites W2074289481 @default.
• W2891997162 cites W2098649929 @default.
• W2891997162 cites W2120799429 @default.
• W2891997162 cites W2131978756 @default.
• W2891997162 cites W2157962408 @default.
• W2891997162 cites W2266017316 @default.
• W2891997162 cites W2270233522 @default.
• W2891997162 cites W2274565482 @default.
• W2891997162 cites W2322000951 @default.
• W2891997162 cites W2398022422 @default.
• W2891997162 cites W2518828749 @default.
• W2891997162 cites W2529491176 @default.
• W2891997162 cites W2551022799 @default.
• W2891997162 cites W2565472650 @default.
• W2891997162 cites W2600380571 @default.
• W2891997162 cites W2606254366 @default.
• W2891997162 cites W2611340938 @default.
• W2891997162 cites W2612525397 @default.
• W2891997162 cites W2614490776 @default.
• W2891997162 cites W2618622436 @default.
• W2891997162 cites W2626580617 @default.
• W2891997162 cites W2736609365 @default.
• W2891997162 cites W2746536920 @default.
• W2891997162 cites W2748201689 @default.
• W2891997162 cites W2751561446 @default.
• W2891997162 cites W2752559852 @default.
• W2891997162 cites W2752743045 @default.
• W2891997162 cites W2754750373 @default.
• W2891997162 cites W2756181794 @default.
• W2891997162 doi "https://doi.org/10.3892/etm.2018.6735" @default.
• W2891997162 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6200966" @default.
• W2891997162 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30402148" @default.
• W2891997162 hasPublicationYear "2018" @default.
• W2891997162 type Work @default.
• W2891997162 sameAs 2891997162 @default.
• W2891997162 citedByCount "5" @default.
• W2891997162 countsByYear W28919971622020 @default.
• W2891997162 countsByYear W28919971622022 @default.
• W2891997162 crossrefType "journal-article" @default.
• W2891997162 hasAuthorship W2891997162A5002661071 @default.
• W2891997162 hasAuthorship W2891997162A5003285644 @default.
• W2891997162 hasAuthorship W2891997162A5023160300 @default.
• W2891997162 hasAuthorship W2891997162A5023613911 @default.
• W2891997162 hasAuthorship W2891997162A5038099693 @default.
• W2891997162 hasAuthorship W2891997162A5050745133 @default.
• W2891997162 hasAuthorship W2891997162A5063395984 @default.
• W2891997162 hasAuthorship W2891997162A5065116828 @default.
• W2891997162 hasAuthorship W2891997162A5073964986 @default.
• W2891997162 hasBestOaLocation W28919971621 @default.
• W2891997162 hasConcept C137620995 @default.
• W2891997162 hasConcept C1491633281 @default.
• W2891997162 hasConcept C153911025 @default.
• W2891997162 hasConcept C160160445 @default.
• W2891997162 hasConcept C181199279 @default.
• W2891997162 hasConcept C185592680 @default.
• W2891997162 hasConcept C198826908 @default.
• W2891997162 hasConcept C2780932548 @default.
• W2891997162 hasConcept C28328180 @default.
• W2891997162 hasConcept C54009773 @default.
• W2891997162 hasConcept C54355233 @default.
• W2891997162 hasConcept C55493867 @default.
• W2891997162 hasConcept C62478195 @default.
• W2891997162 hasConcept C81885089 @default.
• W2891997162 hasConcept C86803240 @default.
• W2891997162 hasConcept C95444343 @default.
• W2891997162 hasConceptScore W2891997162C137620995 @default.
• W2891997162 hasConceptScore W2891997162C1491633281 @default.
• W2891997162 hasConceptScore W2891997162C153911025 @default.
• W2891997162 hasConceptScore W2891997162C160160445 @default.
• W2891997162 hasConceptScore W2891997162C181199279 @default.
• W2891997162 hasConceptScore W2891997162C185592680 @default.
• W2891997162 hasConceptScore W2891997162C198826908 @default.
• W2891997162 hasConceptScore W2891997162C2780932548 @default.
• W2891997162 hasConceptScore W2891997162C28328180 @default.
• W2891997162 hasConceptScore W2891997162C54009773 @default.
• W2891997162 hasConceptScore W2891997162C54355233 @default.
• W2891997162 hasConceptScore W2891997162C55493867 @default.

• next