FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892002176> ?p ?o ?g. }

• W2892002176 abstract "Objective: A Tag Array chip was used to detect plasmids of different template concentration, and then analyzed for sensitivity and specificity. Drug resistance genes from tuberculosis clinical specimens were detected, giving comparative phenotypic resistance results to explore the feasibility and value of clinical applications. Methods: Twenty-four strains of Mycobacterium Tuberculosis (MTB) having sequence differences in extracted plasmids of mutant strains. The plasmid was diluted into different concentrations, and then was performed to analyze the sensitivity and specificity of the chip system. A total of 427 clinical specimens (including spinal tuberculosis and pulmonary tuberculosis) were collected from patients who came from seven hospitals. Design, optimization and preparation of the chip detection system, sequencing and phenotypic drug susceptibility results were analyzed to evaluate the sensitivity and specificity of the gene chip. Results: In the template, concentrations of 1 × 103 copies/μL and above were consistent with sequencing results in the mutant. The sensitivity and specificity in spine Tuberculosis specimen of rifampicin (RFP) were 94.40 and 92.86%; isoniazide (INH) were 92.37 and 87.50%; ethambutol (EMB) were 61.36 and 89.29%; fluoroquinolones (FQS) were 79.41 and 92.86%; streptomycin (SM) were 90.18 and 89.29%; second line drugs (SLD) were 77.61 and 83.93%. In Pulmonary Tuberculosis specimen, the sensitivity and specificity respectively were RFP: 92.74%; 93.75%; INH: 91.26%; 87.50%; EMB: 54.17%; 89.58%; FQS: 84.87%; 93.75%; SM: 86.73%; 85.42%; SLD: 80.9%; 91.67%. The RFP, INH, FQs and SM resistance genes was highly sensitive and specific: however, for detection of amikacin (AMK), capreomycin (CPM), kanamycin (KM), specificity was higher, but sensitivity was lower. Sensitivity for the detection of a mutation in the eis promoter region could be improved. Conclusion: Tag Array chip can achieve rapid, accurate detection of tuberculosis resistance, which has important clinical significance and feasibility." @default.
• W2892002176 created "2018-09-27" @default.
• W2892002176 creator A5002293481 @default.
• W2892002176 creator A5004786498 @default.
• W2892002176 creator A5015337633 @default.
• W2892002176 creator A5022320894 @default.
• W2892002176 creator A5028647898 @default.
• W2892002176 creator A5044895077 @default.
• W2892002176 creator A5063088081 @default.
• W2892002176 creator A5070101287 @default.
• W2892002176 date "2018-09-07" @default.
• W2892002176 modified "2023-09-26" @default.
• W2892002176 title "A Chip for Detecting Tuberculosis Drug Resistance Based on Polymerase Chain Reaction (PCR)-Magnetic Bead Molecule Platform" @default.
• W2892002176 cites W1491655820 @default.
• W2892002176 cites W1610203956 @default.
• W2892002176 cites W1966192968 @default.
• W2892002176 cites W1997237947 @default.
• W2892002176 cites W2001697730 @default.
• W2892002176 cites W2004254509 @default.
• W2892002176 cites W2013585608 @default.
• W2892002176 cites W2015217662 @default.
• W2892002176 cites W2039733501 @default.
• W2892002176 cites W2043226567 @default.
• W2892002176 cites W2072486659 @default.
• W2892002176 cites W2075666807 @default.
• W2892002176 cites W2090070686 @default.
• W2892002176 cites W2101080794 @default.
• W2892002176 cites W2116175177 @default.
• W2892002176 cites W2124243126 @default.
• W2892002176 cites W2124628939 @default.
• W2892002176 cites W2126534483 @default.
• W2892002176 cites W2126621628 @default.
• W2892002176 cites W2128057974 @default.
• W2892002176 cites W2129756442 @default.
• W2892002176 cites W2137758606 @default.
• W2892002176 cites W2138699271 @default.
• W2892002176 cites W2144651829 @default.
• W2892002176 cites W2152075272 @default.
• W2892002176 cites W2153878126 @default.
• W2892002176 cites W2157364615 @default.
• W2892002176 cites W2166566986 @default.
• W2892002176 cites W2342111708 @default.
• W2892002176 cites W4240860361 @default.
• W2892002176 doi "https://doi.org/10.3389/fmicb.2018.02106" @default.
• W2892002176 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6143819" @default.
• W2892002176 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30258420" @default.
• W2892002176 hasPublicationYear "2018" @default.
• W2892002176 type Work @default.
• W2892002176 sameAs 2892002176 @default.
• W2892002176 citedByCount "3" @default.
• W2892002176 countsByYear W28920021762020 @default.
• W2892002176 countsByYear W28920021762023 @default.
• W2892002176 crossrefType "journal-article" @default.
• W2892002176 hasAuthorship W2892002176A5002293481 @default.
• W2892002176 hasAuthorship W2892002176A5004786498 @default.
• W2892002176 hasAuthorship W2892002176A5015337633 @default.
• W2892002176 hasAuthorship W2892002176A5022320894 @default.
• W2892002176 hasAuthorship W2892002176A5028647898 @default.
• W2892002176 hasAuthorship W2892002176A5044895077 @default.
• W2892002176 hasAuthorship W2892002176A5063088081 @default.
• W2892002176 hasAuthorship W2892002176A5070101287 @default.
• W2892002176 hasBestOaLocation W28920021761 @default.
• W2892002176 hasConcept C104317684 @default.
• W2892002176 hasConcept C114851261 @default.
• W2892002176 hasConcept C142724271 @default.
• W2892002176 hasConcept C153911025 @default.
• W2892002176 hasConcept C22744801 @default.
• W2892002176 hasConcept C2775843808 @default.
• W2892002176 hasConcept C2776228421 @default.
• W2892002176 hasConcept C2777975735 @default.
• W2892002176 hasConcept C2778607973 @default.
• W2892002176 hasConcept C2781069245 @default.
• W2892002176 hasConcept C49105822 @default.
• W2892002176 hasConcept C501593827 @default.
• W2892002176 hasConcept C54355233 @default.
• W2892002176 hasConcept C71924100 @default.
• W2892002176 hasConcept C86803240 @default.
• W2892002176 hasConcept C89423630 @default.
• W2892002176 hasConceptScore W2892002176C104317684 @default.
• W2892002176 hasConceptScore W2892002176C114851261 @default.
• W2892002176 hasConceptScore W2892002176C142724271 @default.
• W2892002176 hasConceptScore W2892002176C153911025 @default.
• W2892002176 hasConceptScore W2892002176C22744801 @default.
• W2892002176 hasConceptScore W2892002176C2775843808 @default.
• W2892002176 hasConceptScore W2892002176C2776228421 @default.
• W2892002176 hasConceptScore W2892002176C2777975735 @default.
• W2892002176 hasConceptScore W2892002176C2778607973 @default.
• W2892002176 hasConceptScore W2892002176C2781069245 @default.
• W2892002176 hasConceptScore W2892002176C49105822 @default.
• W2892002176 hasConceptScore W2892002176C501593827 @default.
• W2892002176 hasConceptScore W2892002176C54355233 @default.
• W2892002176 hasConceptScore W2892002176C71924100 @default.
• W2892002176 hasConceptScore W2892002176C86803240 @default.
• W2892002176 hasConceptScore W2892002176C89423630 @default.
• W2892002176 hasLocation W28920021761 @default.
• W2892002176 hasLocation W28920021762 @default.
• W2892002176 hasLocation W28920021763 @default.
• W2892002176 hasLocation W28920021764 @default.
• W2892002176 hasLocation W28920021765 @default.
• W2892002176 hasOpenAccess W2892002176 @default.

• next