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- W2892012109 abstract "Fusarium oxysporum f. sp. canariensis is the causal agent of Fusarium wilt of Canary Island date palm. Fusarium wilt is an important disease that results in the loss of mature palm specimens. Management options involve removing infected trees as quickly as possible to try to prevent spread to other palms. Rapid and accurate detection is vital to the management of this disease. The pathogen is identified with a PCR protocol. We evaluated and modified the detection protocol to correct for false positive results obtained from Fusarium proliferatum which is a common saprophyte on palms. New DNA extraction kits were compared with the previously published extraction technique. Additional PCR primer sets were developed and multiplexed with the previous set to distinguish between the two species. Real-time PCR primers and probes also were evaluated as part of a new detection protocol for the Cepheid SmartCycler system. The new extraction methods and PCRprotocols were evaluated and found to reliably identify F. oxysporum f. sp. canariensis. The real-time PCR protocol also reliably detected the pathogen. The updated protocols distinguished between F. oxysporum f. sp. canariensis, forma specialis other than canariensis, and F. proliferatum. The last chapter of the thesis is a standard operating procedure for diagnosticians who wish to use the updated protocols. ( en )" @default.
- W2892012109 created "2018-09-27" @default.
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- W2892012109 date "2008-01-01" @default.
- W2892012109 modified "2023-09-23" @default.
- W2892012109 title "Development of a Real-Time Polymerase Chain Reaction Diagnostic Technique for Fusarium oxysporum f. sp. canariensis on Palm Species" @default.
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