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- W2892043619 abstract "A novel enzyme-free photoelectrochemical (PEC) immunoassay was developed for the ultrasensitive detection of prostate specific antigen (PSA) based on the DNA-mediated nanoscale zirconium-porphyrin MOFs (NMOFs). By virtue of the intrinsic coordination between unsaturated zirconium sites of the NMOFs frameworks and phosphonate groups, the 5'-phosphorylared ss-DNA-tagged antibody (Ab-DNA) conjugate with a consecutive stretch of guanines as a spacer could be loaded on the NMOFs easily, obtaining a novel type of Ab-DNA-functionalized NMOFs complex. Additionally, as a photocathode PEC active nanomaterial, NMOFs exhibited a significant enhanced photocurrent response with the presence of dopamine under oxygen-containing aqueous media at -0.3 V (vs Ag/AgCl). Furthermore, with the aid of the electrochemical grafting of polyamidoamine (PAMAM) dendrimers functionalized interface, the novel type of Ab-DNA-NMOFs further served as a PEC signal nanoprobe for the ultrasensitive PSA immunoassay. Under optimal conditions, the corresponding immunosensor possessed a wide calibration range of 1 pg mL-1 to 10 ng mL-1 and a limit of detection (LOD) of 0.2 pg mL-1. This present work demonstrated the promising application of DNA-mediated NMOFs in developing highly sensitive, environmentally friendly, and cost-effective PEC biosensors." @default.
- W2892043619 created "2018-09-27" @default.
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- W2892043619 date "2018-09-20" @default.
- W2892043619 modified "2023-10-07" @default.
- W2892043619 title "DNA-Mediated Nanoscale Metal–Organic Frameworks for Ultrasensitive Photoelectrochemical Enzyme-Free Immunoassay" @default.
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- W2892043619 doi "https://doi.org/10.1021/acs.analchem.8b03762" @default.
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