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- W2892047816 abstract "Antibody-fusion proteins with ligands, e.g., of the TNF superfamily (TNFSF) can be adequately produced in mammalian expression systems. Here, we describe the transient production in adherent and suspension human embryonic kidney cells at laboratory scale, followed by purification procedures applying protein A and immobilized metal affinity chromatography for proteins with Fc domain and 6 × histidine-tag, respectively. In addition, characterization of the purified proteins by size exclusion chromatography is described." @default.
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- W2892047816 date "2018-01-01" @default.
- W2892047816 modified "2023-09-27" @default.
- W2892047816 title "Production, Purification, and Characterization of Antibody-TNF Superfamily Ligand Fusion Proteins" @default.
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- W2892047816 doi "https://doi.org/10.1007/978-1-4939-8648-4_18" @default.
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