Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892073350> ?p ?o ?g. }
- W2892073350 endingPage "14084" @default.
- W2892073350 startingPage "14080" @default.
- W2892073350 abstract "Abstract Closthioamide (CTA) is a unique symmetric nonribosomal peptide with six thioamide moieties that is produced by the Gram‐positive obligate anaerobe Ruminiclostridium cellulolyticum . CTA displays potent inhibitory activity against important clinical pathogens, making it a promising drug candidate. Yet, the biosynthesis of this DNA gyrase‐targeting antibiotic has remained enigmatic. Using a combination of genome mining, genome editing (targeted group II intron, CRISPR/Cas9), and heterologous expression, we show that CTA biosynthesis involves specialized enzymes for starter unit biosynthesis, amide bond formation, thionation, and dimerization. Surprisingly, CTA biosynthesis involves a novel thiotemplated peptide assembly line that markedly differs from known nonribosomal peptide synthetases. These findings provide the first insights into the biosynthesis of thioamide‐containing nonribosomal peptides and offer a starting point for the discovery of related natural products." @default.
- W2892073350 created "2018-09-27" @default.
- W2892073350 creator A5032991843 @default.
- W2892073350 creator A5049711516 @default.
- W2892073350 creator A5066230640 @default.
- W2892073350 creator A5073588276 @default.
- W2892073350 creator A5085775144 @default.
- W2892073350 creator A5086918997 @default.
- W2892073350 date "2018-10-03" @default.
- W2892073350 modified "2023-09-25" @default.
- W2892073350 title "Genome Editing Reveals Novel Thiotemplated Assembly of Polythioamide Antibiotics in Anaerobic Bacteria" @default.
- W2892073350 cites W1921225708 @default.
- W2892073350 cites W1940773573 @default.
- W2892073350 cites W1976458443 @default.
- W2892073350 cites W2001189466 @default.
- W2892073350 cites W2027609269 @default.
- W2892073350 cites W2032974626 @default.
- W2892073350 cites W2041262546 @default.
- W2892073350 cites W2049451871 @default.
- W2892073350 cites W2057937871 @default.
- W2892073350 cites W2058259501 @default.
- W2892073350 cites W2067403820 @default.
- W2892073350 cites W2068725665 @default.
- W2892073350 cites W2068902221 @default.
- W2892073350 cites W2076243547 @default.
- W2892073350 cites W2079937579 @default.
- W2892073350 cites W2099047627 @default.
- W2892073350 cites W2101855500 @default.
- W2892073350 cites W2106783740 @default.
- W2892073350 cites W2107141440 @default.
- W2892073350 cites W2110628207 @default.
- W2892073350 cites W2114806975 @default.
- W2892073350 cites W2120656564 @default.
- W2892073350 cites W2121141283 @default.
- W2892073350 cites W2124254997 @default.
- W2892073350 cites W2144252938 @default.
- W2892073350 cites W2154077609 @default.
- W2892073350 cites W2155208047 @default.
- W2892073350 cites W2262475241 @default.
- W2892073350 cites W2414062173 @default.
- W2892073350 cites W2593608868 @default.
- W2892073350 cites W2602307275 @default.
- W2892073350 cites W2605748286 @default.
- W2892073350 cites W2742256737 @default.
- W2892073350 cites W2767339561 @default.
- W2892073350 cites W2785273844 @default.
- W2892073350 cites W2793366789 @default.
- W2892073350 cites W2793464565 @default.
- W2892073350 cites W2810418286 @default.
- W2892073350 cites W4231612347 @default.
- W2892073350 cites W4236745611 @default.
- W2892073350 cites W4241006586 @default.
- W2892073350 cites W4248269532 @default.
- W2892073350 cites W4249525835 @default.
- W2892073350 cites W4256377307 @default.
- W2892073350 doi "https://doi.org/10.1002/anie.201807970" @default.
- W2892073350 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30193003" @default.
- W2892073350 hasPublicationYear "2018" @default.
- W2892073350 type Work @default.
- W2892073350 sameAs 2892073350 @default.
- W2892073350 citedByCount "27" @default.
- W2892073350 countsByYear W28920733502019 @default.
- W2892073350 countsByYear W28920733502020 @default.
- W2892073350 countsByYear W28920733502021 @default.
- W2892073350 countsByYear W28920733502022 @default.
- W2892073350 countsByYear W28920733502023 @default.
- W2892073350 crossrefType "journal-article" @default.
- W2892073350 hasAuthorship W2892073350A5032991843 @default.
- W2892073350 hasAuthorship W2892073350A5049711516 @default.
- W2892073350 hasAuthorship W2892073350A5066230640 @default.
- W2892073350 hasAuthorship W2892073350A5073588276 @default.
- W2892073350 hasAuthorship W2892073350A5085775144 @default.
- W2892073350 hasAuthorship W2892073350A5086918997 @default.
- W2892073350 hasConcept C104317684 @default.
- W2892073350 hasConcept C115574571 @default.
- W2892073350 hasConcept C132141352 @default.
- W2892073350 hasConcept C141231307 @default.
- W2892073350 hasConcept C144501496 @default.
- W2892073350 hasConcept C185592680 @default.
- W2892073350 hasConcept C2777379556 @default.
- W2892073350 hasConcept C2777578258 @default.
- W2892073350 hasConcept C2777650178 @default.
- W2892073350 hasConcept C2909999020 @default.
- W2892073350 hasConcept C523546767 @default.
- W2892073350 hasConcept C54355233 @default.
- W2892073350 hasConcept C54458228 @default.
- W2892073350 hasConcept C553450214 @default.
- W2892073350 hasConcept C55493867 @default.
- W2892073350 hasConcept C64962909 @default.
- W2892073350 hasConcept C67705224 @default.
- W2892073350 hasConcept C70721500 @default.
- W2892073350 hasConcept C71240020 @default.
- W2892073350 hasConcept C86803240 @default.
- W2892073350 hasConcept C88478588 @default.
- W2892073350 hasConceptScore W2892073350C104317684 @default.
- W2892073350 hasConceptScore W2892073350C115574571 @default.
- W2892073350 hasConceptScore W2892073350C132141352 @default.
- W2892073350 hasConceptScore W2892073350C141231307 @default.