Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892087672> ?p ?o ?g. }
- W2892087672 endingPage "3184" @default.
- W2892087672 startingPage "3171" @default.
- W2892087672 abstract "Abstract Modern high‐throughput and high‐content biological research is performed with advanced instrumentation and complex and time‐consuming protocols, which, as a whole, pose a challenge for routine implementation in a research laboratory. In support of a “bioanalytical toolbox” with potential utility for exploring cellular functions mediated via protein phosphorylation‐a post‐translational modification (PTM) with essential regulatory roles in a variety of cellular processes‐in this work, we describe the development of a simple, integrated microfluidic chip that can perform targeted, quantitative analysis of phosphopeptides involved in cancer‐relevant signaling pathways. The microfluidic device comprises microreactors packed with C18 and TiO 2 particles for on‐chip solid phase extraction (SPE) and phosphopeptide enrichment, and an ESI interface for facilitating multiple reaction monitoring (MRM)‐mass spectrometry (MS) detection. The chips are demonstrated for the detection of three phosphopeptides involved in ERBB2/MAPK signaling pathways, selected from the outcome of a proteomic study involving EGF stimulation of SKBR3/HER2+ breast cancer cells. The data demonstrate that the proposed microfluidic strategy can be used for the MS quantification of phosphopeptides in the low nM range from cell lysates without any prior sample pretreatment, fractionation or bioaffinity enrichment, and is generally applicable to the analysis of any phosphopeptide targets." @default.
- W2892087672 created "2018-09-27" @default.
- W2892087672 creator A5006878115 @default.
- W2892087672 creator A5019405139 @default.
- W2892087672 creator A5050559830 @default.
- W2892087672 creator A5087114142 @default.
- W2892087672 date "2018-10-01" @default.
- W2892087672 modified "2023-09-27" @default.
- W2892087672 title "Streamlined microfluidic analysis of phosphopeptides using stable isotope‐labeled synthetic peptides and MRM‐MS detection" @default.
- W2892087672 cites W1490809750 @default.
- W2892087672 cites W1549635624 @default.
- W2892087672 cites W1969378145 @default.
- W2892087672 cites W1969838763 @default.
- W2892087672 cites W1975304761 @default.
- W2892087672 cites W1980947807 @default.
- W2892087672 cites W1983836332 @default.
- W2892087672 cites W1987500408 @default.
- W2892087672 cites W1991361524 @default.
- W2892087672 cites W1997339479 @default.
- W2892087672 cites W1998780144 @default.
- W2892087672 cites W2004441496 @default.
- W2892087672 cites W2012535772 @default.
- W2892087672 cites W2012867417 @default.
- W2892087672 cites W2024923641 @default.
- W2892087672 cites W2029729319 @default.
- W2892087672 cites W2031095666 @default.
- W2892087672 cites W2033413072 @default.
- W2892087672 cites W2033883485 @default.
- W2892087672 cites W2033889320 @default.
- W2892087672 cites W2039136605 @default.
- W2892087672 cites W2051021237 @default.
- W2892087672 cites W2054887015 @default.
- W2892087672 cites W2062289724 @default.
- W2892087672 cites W2072309306 @default.
- W2892087672 cites W2080386769 @default.
- W2892087672 cites W2081150154 @default.
- W2892087672 cites W2099255819 @default.
- W2892087672 cites W2102668719 @default.
- W2892087672 cites W2104764521 @default.
- W2892087672 cites W2107779633 @default.
- W2892087672 cites W2109993774 @default.
- W2892087672 cites W2121103946 @default.
- W2892087672 cites W2133801172 @default.
- W2892087672 cites W2139737762 @default.
- W2892087672 cites W2141091417 @default.
- W2892087672 cites W2150519551 @default.
- W2892087672 cites W2158442654 @default.
- W2892087672 cites W2158969445 @default.
- W2892087672 cites W2169359398 @default.
- W2892087672 cites W2178401034 @default.
- W2892087672 cites W2189178602 @default.
- W2892087672 cites W2238393175 @default.
- W2892087672 cites W2282662039 @default.
- W2892087672 cites W2321570512 @default.
- W2892087672 doi "https://doi.org/10.1002/elps.201800133" @default.
- W2892087672 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30216485" @default.
- W2892087672 hasPublicationYear "2018" @default.
- W2892087672 type Work @default.
- W2892087672 sameAs 2892087672 @default.
- W2892087672 citedByCount "3" @default.
- W2892087672 countsByYear W28920876722019 @default.
- W2892087672 countsByYear W28920876722020 @default.
- W2892087672 crossrefType "journal-article" @default.
- W2892087672 hasAuthorship W2892087672A5006878115 @default.
- W2892087672 hasAuthorship W2892087672A5019405139 @default.
- W2892087672 hasAuthorship W2892087672A5050559830 @default.
- W2892087672 hasAuthorship W2892087672A5087114142 @default.
- W2892087672 hasBestOaLocation W28920876721 @default.
- W2892087672 hasConcept C104317684 @default.
- W2892087672 hasConcept C11960822 @default.
- W2892087672 hasConcept C162356407 @default.
- W2892087672 hasConcept C171250308 @default.
- W2892087672 hasConcept C185592680 @default.
- W2892087672 hasConcept C192562407 @default.
- W2892087672 hasConcept C2779933727 @default.
- W2892087672 hasConcept C34905852 @default.
- W2892087672 hasConcept C43617362 @default.
- W2892087672 hasConcept C46111723 @default.
- W2892087672 hasConcept C55493867 @default.
- W2892087672 hasConcept C6675166 @default.
- W2892087672 hasConcept C8673954 @default.
- W2892087672 hasConcept C87325107 @default.
- W2892087672 hasConcept C97029542 @default.
- W2892087672 hasConceptScore W2892087672C104317684 @default.
- W2892087672 hasConceptScore W2892087672C11960822 @default.
- W2892087672 hasConceptScore W2892087672C162356407 @default.
- W2892087672 hasConceptScore W2892087672C171250308 @default.
- W2892087672 hasConceptScore W2892087672C185592680 @default.
- W2892087672 hasConceptScore W2892087672C192562407 @default.
- W2892087672 hasConceptScore W2892087672C2779933727 @default.
- W2892087672 hasConceptScore W2892087672C34905852 @default.
- W2892087672 hasConceptScore W2892087672C43617362 @default.
- W2892087672 hasConceptScore W2892087672C46111723 @default.
- W2892087672 hasConceptScore W2892087672C55493867 @default.
- W2892087672 hasConceptScore W2892087672C6675166 @default.
- W2892087672 hasConceptScore W2892087672C8673954 @default.
- W2892087672 hasConceptScore W2892087672C87325107 @default.
- W2892087672 hasConceptScore W2892087672C97029542 @default.