Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892097320> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2892097320 endingPage "129" @default.
- W2892097320 startingPage "124" @default.
- W2892097320 abstract "It is recognized that ovarian follicular atresia is associated with apoptosis, and the most important effector of cell death is caspase-3. The aim of this study was to investigate the influence of anti-apoptotic drug Z-VAD-FMK on in vitro follicle growth in the domestic dog. Ovaries were obtained from peri-pubertal and adult domestic dogs, and cortical fragments recovered and incubated on 1.5% (w/v) agarose gel blocks within a 24-well culture plate containing Minimum Essential Medium Eagle-Alpha Modification (αMEM) supplemented with 4.2 μg/mL insulin, 3.8 μg/mL transferrin, 5 ng/mL selenium, 2 mM L-glutamine, 100 μg/mL of penicillin G sodium, 100 μg/mL of streptomycin sulfate, 0.05 mM ascorbic acid, 10 ng/mL of FSH and 0.1% (w/v) polyvinyl alcohol in humidified atmosphere of 5% CO2 and 5% O2. The cortices were randomly allocated in six treatments: 1) 10 ng/mL EGF (EGF V0); 2) 10 ng/mL of EGF plus 1 mM Z-VAD-FMK (EGF V1); 3) 10 ng/mL of EGF and 10 mM Z-VAD-FMK (EGF V10); 4) 1 mM Z-VAD-FMK; 5) 10 mM Z-VAD-FMK and (6) no EGF and Z-VAD-FMK supplementation (Control). The cortices were processed for histology and assessed for viability (based on morphology), density of structurally normal follicles, and diameter immediately after collection (non-culture Control) or after 3 or 7 days of in vitro incubation. Evaluation of mRNA expression of Cas3 in fresh cortices and those incubated for 3 days was performed using real-time PCR. Histological analysis revealed that in vitro incubation decreased (P < 0.05) follicle viability and density compared to the fresh, non-culture control. Addition of 10 μM of Z-VAD-FMK alone to the culture medium sustained follicle viability at Day 3, but did not impact follicle diameter when compared to the other treatment groups (p < 0.001); however, the beneficial benefit of this anti-apoptotic drug diminished after 7 days of incubation. Furthermore, Z-VAD-FMK supplementation did not impact Cas3 expression. The findings demonstrated that dog ovarian tissues are highly susceptible to in vitro incubation and Z-VAD-FMK supported short-term survival of dog follicles enclosed within the ovarian cortex." @default.
- W2892097320 created "2018-09-27" @default.
- W2892097320 creator A5012675694 @default.
- W2892097320 creator A5067432745 @default.
- W2892097320 creator A5069891195 @default.
- W2892097320 creator A5070626316 @default.
- W2892097320 date "2018-12-01" @default.
- W2892097320 modified "2023-10-14" @default.
- W2892097320 title "Effect of anti-apoptotic drug Z-VAD-FMK on in vitro viability of dog follicles" @default.
- W2892097320 cites W2006655933 @default.
- W2892097320 cites W2021759930 @default.
- W2892097320 cites W2032515494 @default.
- W2892097320 cites W2037518090 @default.
- W2892097320 cites W2041116159 @default.
- W2892097320 cites W2047527040 @default.
- W2892097320 cites W2062596593 @default.
- W2892097320 cites W2073489666 @default.
- W2892097320 cites W2078307923 @default.
- W2892097320 cites W2078637554 @default.
- W2892097320 cites W2092208552 @default.
- W2892097320 cites W2093840172 @default.
- W2892097320 cites W2100247566 @default.
- W2892097320 cites W2123953793 @default.
- W2892097320 cites W2125017552 @default.
- W2892097320 cites W2129890012 @default.
- W2892097320 cites W2138268960 @default.
- W2892097320 cites W2142206986 @default.
- W2892097320 cites W2142250901 @default.
- W2892097320 cites W2203906945 @default.
- W2892097320 cites W2216557758 @default.
- W2892097320 cites W2528762358 @default.
- W2892097320 cites W2550605817 @default.
- W2892097320 cites W2773301741 @default.
- W2892097320 cites W2786090367 @default.
- W2892097320 cites W4251881900 @default.
- W2892097320 doi "https://doi.org/10.1016/j.theriogenology.2018.09.012" @default.
- W2892097320 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30248613" @default.
- W2892097320 hasPublicationYear "2018" @default.
- W2892097320 type Work @default.
- W2892097320 sameAs 2892097320 @default.
- W2892097320 citedByCount "1" @default.
- W2892097320 countsByYear W28920973202023 @default.
- W2892097320 crossrefType "journal-article" @default.
- W2892097320 hasAuthorship W2892097320A5012675694 @default.
- W2892097320 hasAuthorship W2892097320A5067432745 @default.
- W2892097320 hasAuthorship W2892097320A5069891195 @default.
- W2892097320 hasAuthorship W2892097320A5070626316 @default.
- W2892097320 hasBestOaLocation W28920973201 @default.
- W2892097320 hasConcept C134018914 @default.
- W2892097320 hasConcept C153911025 @default.
- W2892097320 hasConcept C16685009 @default.
- W2892097320 hasConcept C185592680 @default.
- W2892097320 hasConcept C190283241 @default.
- W2892097320 hasConcept C2779349466 @default.
- W2892097320 hasConcept C515207424 @default.
- W2892097320 hasConcept C53227056 @default.
- W2892097320 hasConcept C55493867 @default.
- W2892097320 hasConcept C71924100 @default.
- W2892097320 hasConcept C86803240 @default.
- W2892097320 hasConceptScore W2892097320C134018914 @default.
- W2892097320 hasConceptScore W2892097320C153911025 @default.
- W2892097320 hasConceptScore W2892097320C16685009 @default.
- W2892097320 hasConceptScore W2892097320C185592680 @default.
- W2892097320 hasConceptScore W2892097320C190283241 @default.
- W2892097320 hasConceptScore W2892097320C2779349466 @default.
- W2892097320 hasConceptScore W2892097320C515207424 @default.
- W2892097320 hasConceptScore W2892097320C53227056 @default.
- W2892097320 hasConceptScore W2892097320C55493867 @default.
- W2892097320 hasConceptScore W2892097320C71924100 @default.
- W2892097320 hasConceptScore W2892097320C86803240 @default.
- W2892097320 hasFunder F4320306083 @default.
- W2892097320 hasFunder F4320320997 @default.
- W2892097320 hasLocation W28920973201 @default.
- W2892097320 hasLocation W28920973202 @default.
- W2892097320 hasOpenAccess W2892097320 @default.
- W2892097320 hasPrimaryLocation W28920973201 @default.
- W2892097320 hasRelatedWork W2347359022 @default.
- W2892097320 hasRelatedWork W2351776519 @default.
- W2892097320 hasRelatedWork W2360919160 @default.
- W2892097320 hasRelatedWork W2363571182 @default.
- W2892097320 hasRelatedWork W2370207786 @default.
- W2892097320 hasRelatedWork W2381004937 @default.
- W2892097320 hasRelatedWork W2386832033 @default.
- W2892097320 hasRelatedWork W2748952813 @default.
- W2892097320 hasRelatedWork W4289223126 @default.
- W2892097320 hasRelatedWork W1860850171 @default.
- W2892097320 hasVolume "122" @default.
- W2892097320 isParatext "false" @default.
- W2892097320 isRetracted "false" @default.
- W2892097320 magId "2892097320" @default.
- W2892097320 workType "article" @default.