Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892173888> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2892173888 abstract "Extracellular vesicles (EV) have attracted attention as circulating biomarkers for many diseases, particularly cancer. Conventional immunofluorescence staining has been used for the detection of target antigens on EV by flow cytometry. However, the staining intensity depends on the amount of antigen expressed on the vesicles and is often only around the noise level. Instead of immunofluorescence, we combined immunomagnetic separation using nanosize MACS® MicroBeads with phospholipid staining of EV (IMS-PS method). EpCAM-positive EV were prepared from the culture supernatants of OVCAR3 (EpCAM-high), A431 (EpCAM-low) or Colon-26 (non-human control) cells as cancer models and were examined by the IMS-PS method using EpCAM mAb-coated MicroBeads. By employing Polaric-500c6F as the dye for staining EV phospholipids and using appropriate flow cytometry settings, autofluorescence was excluded, whereas pretreatment of the MicroBeads with conventional blocking agents reduced nonspecific binding to non-target vesicles. These modifications resulted in a linear relation between the number of EV detected and the sample volume, regardless of the level of EpCAM expression on the vesicles. A431 EV spiked into healthy volunteer plasma were enumerated with good accuracy. The IMS-PS method may be useful for clinical evaluation of EV with low levels of antigen expression that are difficult to detect by conventional immunofluorescence." @default.
- W2892173888 created "2018-09-27" @default.
- W2892173888 creator A5007379824 @default.
- W2892173888 creator A5011452278 @default.
- W2892173888 creator A5048907152 @default.
- W2892173888 creator A5068370626 @default.
- W2892173888 date "2018-10-24" @default.
- W2892173888 modified "2023-09-24" @default.
- W2892173888 title "Flow cytometric quantitation of EpCAM-positive extracellular vesicles by immunomagnetic separation and phospholipid staining method" @default.
- W2892173888 cites W1596808825 @default.
- W2892173888 cites W1871390418 @default.
- W2892173888 cites W1917937260 @default.
- W2892173888 cites W1994344811 @default.
- W2892173888 cites W1997122898 @default.
- W2892173888 cites W2009625495 @default.
- W2892173888 cites W2012295691 @default.
- W2892173888 cites W2012454696 @default.
- W2892173888 cites W2018191093 @default.
- W2892173888 cites W2048452946 @default.
- W2892173888 cites W2054410308 @default.
- W2892173888 cites W2072813859 @default.
- W2892173888 cites W2074891817 @default.
- W2892173888 cites W2086016395 @default.
- W2892173888 cites W2088851260 @default.
- W2892173888 cites W2121354606 @default.
- W2892173888 cites W2169505041 @default.
- W2892173888 cites W2191816501 @default.
- W2892173888 cites W2343797741 @default.
- W2892173888 cites W2474977599 @default.
- W2892173888 cites W2533480443 @default.
- W2892173888 cites W2549458217 @default.
- W2892173888 cites W2552535243 @default.
- W2892173888 cites W2582281019 @default.
- W2892173888 cites W2584631915 @default.
- W2892173888 cites W2612774616 @default.
- W2892173888 cites W2617507069 @default.
- W2892173888 cites W2751344545 @default.
- W2892173888 cites W2764019325 @default.
- W2892173888 cites W2805560621 @default.
- W2892173888 doi "https://doi.org/10.1111/gtc.12645" @default.
- W2892173888 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30220098" @default.
- W2892173888 hasPublicationYear "2018" @default.
- W2892173888 type Work @default.
- W2892173888 sameAs 2892173888 @default.
- W2892173888 citedByCount "5" @default.
- W2892173888 countsByYear W28921738882019 @default.
- W2892173888 countsByYear W28921738882020 @default.
- W2892173888 countsByYear W28921738882022 @default.
- W2892173888 countsByYear W28921738882023 @default.
- W2892173888 crossrefType "journal-article" @default.
- W2892173888 hasAuthorship W2892173888A5007379824 @default.
- W2892173888 hasAuthorship W2892173888A5011452278 @default.
- W2892173888 hasAuthorship W2892173888A5048907152 @default.
- W2892173888 hasAuthorship W2892173888A5068370626 @default.
- W2892173888 hasBestOaLocation W28921738881 @default.
- W2892173888 hasConcept C130316041 @default.
- W2892173888 hasConcept C147483822 @default.
- W2892173888 hasConcept C153911025 @default.
- W2892173888 hasConcept C159654299 @default.
- W2892173888 hasConcept C203014093 @default.
- W2892173888 hasConcept C2778236600 @default.
- W2892173888 hasConcept C2781053203 @default.
- W2892173888 hasConcept C41625074 @default.
- W2892173888 hasConcept C54355233 @default.
- W2892173888 hasConcept C553184892 @default.
- W2892173888 hasConcept C55493867 @default.
- W2892173888 hasConcept C74864618 @default.
- W2892173888 hasConcept C86803240 @default.
- W2892173888 hasConceptScore W2892173888C130316041 @default.
- W2892173888 hasConceptScore W2892173888C147483822 @default.
- W2892173888 hasConceptScore W2892173888C153911025 @default.
- W2892173888 hasConceptScore W2892173888C159654299 @default.
- W2892173888 hasConceptScore W2892173888C203014093 @default.
- W2892173888 hasConceptScore W2892173888C2778236600 @default.
- W2892173888 hasConceptScore W2892173888C2781053203 @default.
- W2892173888 hasConceptScore W2892173888C41625074 @default.
- W2892173888 hasConceptScore W2892173888C54355233 @default.
- W2892173888 hasConceptScore W2892173888C553184892 @default.
- W2892173888 hasConceptScore W2892173888C55493867 @default.
- W2892173888 hasConceptScore W2892173888C74864618 @default.
- W2892173888 hasConceptScore W2892173888C86803240 @default.
- W2892173888 hasFunder F4320323584 @default.
- W2892173888 hasLocation W28921738881 @default.
- W2892173888 hasLocation W28921738882 @default.
- W2892173888 hasOpenAccess W2892173888 @default.
- W2892173888 hasPrimaryLocation W28921738881 @default.
- W2892173888 hasRelatedWork W1495540074 @default.
- W2892173888 hasRelatedWork W1984629061 @default.
- W2892173888 hasRelatedWork W1985943124 @default.
- W2892173888 hasRelatedWork W1989289676 @default.
- W2892173888 hasRelatedWork W2023775957 @default.
- W2892173888 hasRelatedWork W2063795624 @default.
- W2892173888 hasRelatedWork W2073262237 @default.
- W2892173888 hasRelatedWork W2254251806 @default.
- W2892173888 hasRelatedWork W2375758616 @default.
- W2892173888 hasRelatedWork W4250557310 @default.
- W2892173888 isParatext "false" @default.
- W2892173888 isRetracted "false" @default.
- W2892173888 magId "2892173888" @default.
- W2892173888 workType "article" @default.