FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892224178> ?p ?o ?g. }

• W2892224178 abstract "Presynaptic cGMP-gated ion (CNG) channels positively or negatively modulate neurotransmitter secretion as well as the strength of synaptic transmission. Zebrafish cGMP-gated ion channel, CNGA2a (a.k.a. CNGA5), was previously reported to be specifically enriched in synaptic terminals of zebrafish oxytocin (OXT) neurons. This conclusion was based on immunoreactivity of a monoclonal antibody (mAb) clone L55/54, which was directed against the carboxy terminal tail of the CNGA2a. To study the role of CNGA2a in oxytocin neurons function, we generated zebrafish mutants of cnga2a, cnga2b and oxytocin (oxt) genes using CRISPR-mediated genome editing. We show that mAb L55/54 specifically recognizes CNGA2a protein when expressed in heterologous cell culture system. Surprisingly, anti-CNGA2a immunoreactivity was not eliminated following knockout of either cnga2a, cnga2b or both. However, knockout of oxt resulted in total loss of anti-CNGA2a mAb immunoreactivity despite the lack of sequence and structural similarities between OXT and CNGA2a proteins. Our results provide a noteworthy lesson of differences in antibody immunoreactivity, which could only be revealed using specific genetic tools." @default.
• W2892224178 created "2018-09-27" @default.
• W2892224178 creator A5000166145 @default.
• W2892224178 creator A5020737959 @default.
• W2892224178 creator A5026281812 @default.
• W2892224178 creator A5028652571 @default.
• W2892224178 date "2018-09-20" @default.
• W2892224178 modified "2023-10-06" @default.
• W2892224178 title "Genome Editing Reveals Idiosyncrasy of CNGA2 Ion Channel-Directed Antibody Immunoreactivity Toward Oxytocin" @default.
• W2892224178 cites W1748779381 @default.
• W2892224178 cites W1872459230 @default.
• W2892224178 cites W1887864014 @default.
• W2892224178 cites W1964277253 @default.
• W2892224178 cites W1969737958 @default.
• W2892224178 cites W1989062378 @default.
• W2892224178 cites W2001486453 @default.
• W2892224178 cites W2004798786 @default.
• W2892224178 cites W2009810462 @default.
• W2892224178 cites W2011617296 @default.
• W2892224178 cites W2018980625 @default.
• W2892224178 cites W2020556204 @default.
• W2892224178 cites W2032226763 @default.
• W2892224178 cites W2032967567 @default.
• W2892224178 cites W2034342718 @default.
• W2892224178 cites W2035584685 @default.
• W2892224178 cites W2040119784 @default.
• W2892224178 cites W2058283488 @default.
• W2892224178 cites W2092254253 @default.
• W2892224178 cites W2093062941 @default.
• W2892224178 cites W2098094063 @default.
• W2892224178 cites W2098290298 @default.
• W2892224178 cites W2107862694 @default.
• W2892224178 cites W2117764478 @default.
• W2892224178 cites W2120309756 @default.
• W2892224178 cites W2161615619 @default.
• W2892224178 cites W2162685663 @default.
• W2892224178 cites W2164695230 @default.
• W2892224178 cites W2167652900 @default.
• W2892224178 cites W2419493891 @default.
• W2892224178 cites W2491618709 @default.
• W2892224178 cites W2578984598 @default.
• W2892224178 doi "https://doi.org/10.3389/fcell.2018.00117" @default.
• W2892224178 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6158348" @default.
• W2892224178 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30294598" @default.
• W2892224178 hasPublicationYear "2018" @default.
• W2892224178 type Work @default.
• W2892224178 sameAs 2892224178 @default.
• W2892224178 citedByCount "11" @default.
• W2892224178 countsByYear W28922241782019 @default.
• W2892224178 countsByYear W28922241782020 @default.
• W2892224178 countsByYear W28922241782021 @default.
• W2892224178 countsByYear W28922241782022 @default.
• W2892224178 countsByYear W28922241782023 @default.
• W2892224178 crossrefType "journal-article" @default.
• W2892224178 hasAuthorship W2892224178A5000166145 @default.
• W2892224178 hasAuthorship W2892224178A5020737959 @default.
• W2892224178 hasAuthorship W2892224178A5026281812 @default.
• W2892224178 hasAuthorship W2892224178A5028652571 @default.
• W2892224178 hasBestOaLocation W28922241781 @default.
• W2892224178 hasConcept C104317684 @default.
• W2892224178 hasConcept C159654299 @default.
• W2892224178 hasConcept C169760540 @default.
• W2892224178 hasConcept C170493617 @default.
• W2892224178 hasConcept C173396325 @default.
• W2892224178 hasConcept C2776176026 @default.
• W2892224178 hasConcept C2776878037 @default.
• W2892224178 hasConcept C50254741 @default.
• W2892224178 hasConcept C542903549 @default.
• W2892224178 hasConcept C54355233 @default.
• W2892224178 hasConcept C86803240 @default.
• W2892224178 hasConcept C95444343 @default.
• W2892224178 hasConceptScore W2892224178C104317684 @default.
• W2892224178 hasConceptScore W2892224178C159654299 @default.
• W2892224178 hasConceptScore W2892224178C169760540 @default.
• W2892224178 hasConceptScore W2892224178C170493617 @default.
• W2892224178 hasConceptScore W2892224178C173396325 @default.
• W2892224178 hasConceptScore W2892224178C2776176026 @default.
• W2892224178 hasConceptScore W2892224178C2776878037 @default.
• W2892224178 hasConceptScore W2892224178C50254741 @default.
• W2892224178 hasConceptScore W2892224178C542903549 @default.
• W2892224178 hasConceptScore W2892224178C54355233 @default.
• W2892224178 hasConceptScore W2892224178C86803240 @default.
• W2892224178 hasConceptScore W2892224178C95444343 @default.
• W2892224178 hasFunder F4320320878 @default.
• W2892224178 hasFunder F4320322252 @default.
• W2892224178 hasLocation W28922241781 @default.
• W2892224178 hasLocation W28922241782 @default.
• W2892224178 hasLocation W28922241783 @default.
• W2892224178 hasLocation W28922241784 @default.
• W2892224178 hasOpenAccess W2892224178 @default.
• W2892224178 hasPrimaryLocation W28922241781 @default.
• W2892224178 hasRelatedWork W142519857 @default.
• W2892224178 hasRelatedWork W162930727 @default.
• W2892224178 hasRelatedWork W1984891187 @default.
• W2892224178 hasRelatedWork W2005400476 @default.
• W2892224178 hasRelatedWork W2067778076 @default.
• W2892224178 hasRelatedWork W2616544231 @default.
• W2892224178 hasRelatedWork W2784285100 @default.
• W2892224178 hasRelatedWork W2809992820 @default.
• W2892224178 hasRelatedWork W2896335249 @default.

• next