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- W2892230668 abstract "The existence of G protein-dependent and -independent mechanisms activated by sodium fluoride was examined in muscle cells isolated separately from the circular and longitudinal layers of guinea pig intestine. The cells were transiently permeabilized by incubation with Trans.Port Reagent in the presence or absence of GDPβS (100 μM) and then re-sealed. In the absence of GDPβS, NaF (1 mM) induced contraction and caused an increase in [Ca2+]i, IP3 and diacylglycerol levels and in protein kinase C (PKC) activity in both cell types. In the presence of GDPβS, the increases in IP3, DAG and PKC were abolished whereas contraction and the increase in [Ca2+]i were partly inhibited. Residual contraction and [Ca2+]i were abolished by the Ca2+ channel blocker, methoxyverapamil. We conclude that contraction and Ca2+ mobilization induced by NaF is mediated by G protein activation as well as by a G protein-independent mechanism involving activation of plasmalemmal Ca2+ channels." @default.
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- W2892230668 date "2016-10-01" @default.
- W2892230668 modified "2023-09-25" @default.
- W2892230668 title "Differences in effects of NMDA receptor antagonists in BARR2-KO mice" @default.
- W2892230668 doi "https://doi.org/10.1016/s0924-977x(16)31163-4" @default.
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