Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892263222> ?p ?o ?g. }
- W2892263222 abstract "Exosomes have emerged as important mediators of intercellular communication. Although their modes of action have been elucidated, the molecular mechanisms underlying their secretion, sorting of molecules, uptake into recipient cells, and biological distribution in vivo remain elusive. Here, we present a novel system for quantifying secreted exosomes by introducing ectopic or CRISPR/Cas9-mediated knock-in of luciferase-fusion exosome markers such as CD63. This luciferase-based method makes it possible to measure exosomes secreted into the culture medium with high linearity and wide dynamic range in a high-throughput manner. We demonstrate that data obtained by luminescent quantification are well correlated with data obtained by conventional nanoparticle tracking analysis under multiple conditions. In addition, our system is capable of evaluating the recipient cells or tissues that take up exosomes, as well as visualizing exosomes in vivo. The proposed system represents a powerful tool for understanding the molecular mechanisms underlying exosome production, uptake, and long-term distribution." @default.
- W2892263222 created "2018-09-27" @default.
- W2892263222 creator A5022946411 @default.
- W2892263222 creator A5023388437 @default.
- W2892263222 creator A5036594249 @default.
- W2892263222 creator A5048390097 @default.
- W2892263222 date "2018-09-19" @default.
- W2892263222 modified "2023-10-17" @default.
- W2892263222 title "Sensitive and rapid quantification of exosomes by fusing luciferase to exosome marker proteins" @default.
- W2892263222 cites W1500925570 @default.
- W2892263222 cites W1508249887 @default.
- W2892263222 cites W1591311438 @default.
- W2892263222 cites W1798331267 @default.
- W2892263222 cites W1872071454 @default.
- W2892263222 cites W1890513212 @default.
- W2892263222 cites W1944985511 @default.
- W2892263222 cites W1967367827 @default.
- W2892263222 cites W1970877840 @default.
- W2892263222 cites W1974505166 @default.
- W2892263222 cites W1981012538 @default.
- W2892263222 cites W1985543845 @default.
- W2892263222 cites W1987409881 @default.
- W2892263222 cites W1992970284 @default.
- W2892263222 cites W1995136218 @default.
- W2892263222 cites W1998336649 @default.
- W2892263222 cites W1998661425 @default.
- W2892263222 cites W2000455734 @default.
- W2892263222 cites W2022805251 @default.
- W2892263222 cites W2025199750 @default.
- W2892263222 cites W2027195751 @default.
- W2892263222 cites W2028006609 @default.
- W2892263222 cites W2034774921 @default.
- W2892263222 cites W2038363115 @default.
- W2892263222 cites W2055313665 @default.
- W2892263222 cites W2055791162 @default.
- W2892263222 cites W2057332675 @default.
- W2892263222 cites W2060133690 @default.
- W2892263222 cites W2061229454 @default.
- W2892263222 cites W2076266324 @default.
- W2892263222 cites W2078969239 @default.
- W2892263222 cites W2084271408 @default.
- W2892263222 cites W2087008556 @default.
- W2892263222 cites W2097343453 @default.
- W2892263222 cites W2108057472 @default.
- W2892263222 cites W2121725913 @default.
- W2892263222 cites W2126210405 @default.
- W2892263222 cites W2131409719 @default.
- W2892263222 cites W2154203178 @default.
- W2892263222 cites W2259481044 @default.
- W2892263222 cites W2281134225 @default.
- W2892263222 cites W2301890235 @default.
- W2892263222 cites W2522821491 @default.
- W2892263222 cites W2554427090 @default.
- W2892263222 cites W2568845480 @default.
- W2892263222 cites W2608932173 @default.
- W2892263222 cites W2736321686 @default.
- W2892263222 cites W2750925568 @default.
- W2892263222 cites W2765293311 @default.
- W2892263222 cites W2770146439 @default.
- W2892263222 cites W2783944216 @default.
- W2892263222 cites W2790701366 @default.
- W2892263222 cites W2793044374 @default.
- W2892263222 cites W2796493184 @default.
- W2892263222 cites W2802794566 @default.
- W2892263222 cites W4210471029 @default.
- W2892263222 doi "https://doi.org/10.1038/s41598-018-32535-7" @default.
- W2892263222 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6145919" @default.
- W2892263222 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30232365" @default.
- W2892263222 hasPublicationYear "2018" @default.
- W2892263222 type Work @default.
- W2892263222 sameAs 2892263222 @default.
- W2892263222 citedByCount "63" @default.
- W2892263222 countsByYear W28922632222019 @default.
- W2892263222 countsByYear W28922632222020 @default.
- W2892263222 countsByYear W28922632222021 @default.
- W2892263222 countsByYear W28922632222022 @default.
- W2892263222 countsByYear W28922632222023 @default.
- W2892263222 crossrefType "journal-article" @default.
- W2892263222 hasAuthorship W2892263222A5022946411 @default.
- W2892263222 hasAuthorship W2892263222A5023388437 @default.
- W2892263222 hasAuthorship W2892263222A5036594249 @default.
- W2892263222 hasAuthorship W2892263222A5048390097 @default.
- W2892263222 hasBestOaLocation W28922632221 @default.
- W2892263222 hasConcept C104317684 @default.
- W2892263222 hasConcept C111335760 @default.
- W2892263222 hasConcept C145059251 @default.
- W2892263222 hasConcept C185592680 @default.
- W2892263222 hasConcept C20518536 @default.
- W2892263222 hasConcept C207001950 @default.
- W2892263222 hasConcept C2778120352 @default.
- W2892263222 hasConcept C2781261824 @default.
- W2892263222 hasConcept C49039625 @default.
- W2892263222 hasConcept C54009773 @default.
- W2892263222 hasConcept C54355233 @default.
- W2892263222 hasConcept C55493867 @default.
- W2892263222 hasConcept C70721500 @default.
- W2892263222 hasConcept C81885089 @default.
- W2892263222 hasConcept C86803240 @default.
- W2892263222 hasConcept C95444343 @default.
- W2892263222 hasConcept C98108389 @default.