FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892266199> ?p ?o ?g. }

• W2892266199 endingPage "e0198862" @default.
• W2892266199 startingPage "e0198862" @default.
• W2892266199 abstract "Keratinocytes are the most abundant cell type in the epidermis. They prevent desiccation and provide immunological and barrier defense against potential pathogens such as Staphylococcus aureus and Candida albicans. The study of this first line of immune defense may be hindered by invasive isolation methods and/or improper culture conditions to support stem cell maintenance and other potential mechanisms contributing to long-term subcultivation in vitro. Primary keratinocytes have been successfully isolated from blister roofs induced by negative pressure, which separates the epidermis from the dermis in vivo in human subjects. This method allows collection of pure epidermal cells without dermal contamination in a minimally invasive manner. However, the isolated keratinocytes differentiate and senesce when cultured in vitro beyond five passages. Here, we present evidence that the Rho kinase (ROCK) inhibitor Y-27632 can be used to effectively increase the proliferative capabilities of keratinocytes isolated using the suction blister method, similar to what has been previously reported for primary keratinocytes isolated using alternative methods. We show that the increase in passage number is directly correlated to delayed differentiation, and that cells passaged long term with the inhibitor retain their ability to stratify in organotypic raft cultures and respond to cytokine treatment; additionally, the late passage cells have a heterogeneous mix of differentiated and non-differentiated cells which may be predicted by a ratio of select differentiation markers. The described method presents a minimally invasive procedure for keratinocyte isolation and prolonged culture that allows analysis of keratinocyte function in both healthy volunteers and patients with dermatologic diseases." @default.
• W2892266199 created "2018-09-27" @default.
• W2892266199 creator A5003188767 @default.
• W2892266199 creator A5007400359 @default.
• W2892266199 creator A5012745489 @default.
• W2892266199 creator A5018818205 @default.
• W2892266199 creator A5027258238 @default.
• W2892266199 creator A5050609317 @default.
• W2892266199 creator A5051121734 @default.
• W2892266199 creator A5061592542 @default.
• W2892266199 creator A5068961940 @default.
• W2892266199 creator A5085809688 @default.
• W2892266199 date "2018-09-12" @default.
• W2892266199 modified "2023-10-06" @default.
• W2892266199 title "Prolonging culture of primary human keratinocytes isolated from suction blisters with the Rho kinase inhibitor Y-27632" @default.
• W2892266199 cites W1512513473 @default.
• W2892266199 cites W1597787921 @default.
• W2892266199 cites W1963510280 @default.
• W2892266199 cites W1976134177 @default.
• W2892266199 cites W1989224577 @default.
• W2892266199 cites W1994736072 @default.
• W2892266199 cites W1998914462 @default.
• W2892266199 cites W2005356516 @default.
• W2892266199 cites W2008029604 @default.
• W2892266199 cites W2008381030 @default.
• W2892266199 cites W2013108964 @default.
• W2892266199 cites W2016693266 @default.
• W2892266199 cites W2021288989 @default.
• W2892266199 cites W2022753971 @default.
• W2892266199 cites W2037749974 @default.
• W2892266199 cites W2037801769 @default.
• W2892266199 cites W2039909286 @default.
• W2892266199 cites W2049043712 @default.
• W2892266199 cites W2061466699 @default.
• W2892266199 cites W2076771588 @default.
• W2892266199 cites W2077603983 @default.
• W2892266199 cites W2084753863 @default.
• W2892266199 cites W2111344666 @default.
• W2892266199 cites W2120685230 @default.
• W2892266199 cites W2126432370 @default.
• W2892266199 cites W2148569723 @default.
• W2892266199 cites W2169096873 @default.
• W2892266199 cites W2328859818 @default.
• W2892266199 cites W2404939041 @default.
• W2892266199 cites W2405135753 @default.
• W2892266199 cites W2408881078 @default.
• W2892266199 cites W2466443795 @default.
• W2892266199 cites W2581675136 @default.
• W2892266199 cites W2791272535 @default.
• W2892266199 cites W2798067203 @default.
• W2892266199 cites W2804222503 @default.
• W2892266199 cites W50175125 @default.
• W2892266199 doi "https://doi.org/10.1371/journal.pone.0198862" @default.
• W2892266199 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6135349" @default.
• W2892266199 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30208113" @default.
• W2892266199 hasPublicationYear "2018" @default.
• W2892266199 type Work @default.
• W2892266199 sameAs 2892266199 @default.
• W2892266199 citedByCount "10" @default.
• W2892266199 countsByYear W28922661992019 @default.
• W2892266199 countsByYear W28922661992020 @default.
• W2892266199 countsByYear W28922661992021 @default.
• W2892266199 countsByYear W28922661992022 @default.
• W2892266199 countsByYear W28922661992023 @default.
• W2892266199 crossrefType "journal-article" @default.
• W2892266199 hasAuthorship W2892266199A5003188767 @default.
• W2892266199 hasAuthorship W2892266199A5007400359 @default.
• W2892266199 hasAuthorship W2892266199A5012745489 @default.
• W2892266199 hasAuthorship W2892266199A5018818205 @default.
• W2892266199 hasAuthorship W2892266199A5027258238 @default.
• W2892266199 hasAuthorship W2892266199A5050609317 @default.
• W2892266199 hasAuthorship W2892266199A5051121734 @default.
• W2892266199 hasAuthorship W2892266199A5061592542 @default.
• W2892266199 hasAuthorship W2892266199A5068961940 @default.
• W2892266199 hasAuthorship W2892266199A5085809688 @default.
• W2892266199 hasBestOaLocation W28922661991 @default.
• W2892266199 hasConcept C102230213 @default.
• W2892266199 hasConcept C104317684 @default.
• W2892266199 hasConcept C105702510 @default.
• W2892266199 hasConcept C141035611 @default.
• W2892266199 hasConcept C202751555 @default.
• W2892266199 hasConcept C203014093 @default.
• W2892266199 hasConcept C2776458125 @default.
• W2892266199 hasConcept C2777074287 @default.
• W2892266199 hasConcept C2777624698 @default.
• W2892266199 hasConcept C2778266373 @default.
• W2892266199 hasConcept C2778962987 @default.
• W2892266199 hasConcept C2780917455 @default.
• W2892266199 hasConcept C2780949179 @default.
• W2892266199 hasConcept C28328180 @default.
• W2892266199 hasConcept C51911345 @default.
• W2892266199 hasConcept C54355233 @default.
• W2892266199 hasConcept C55493867 @default.
• W2892266199 hasConcept C81885089 @default.
• W2892266199 hasConcept C86803240 @default.
• W2892266199 hasConcept C89423630 @default.
• W2892266199 hasConcept C95444343 @default.
• W2892266199 hasConceptScore W2892266199C102230213 @default.

• next