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- W2892289766 abstract "We introduce SPADE (small particle detection), a novel method to detect and characterize objects only a few pixel large on microscopy images, based on the use of a dictionary of shapes (Figure 1). It involves the minimization of an energy function embedding a prior and a data fidelity term to match shapes of the dictionary with patterns in images. The data fidelity term is a comparison between the pixels' intensities within a potential match and the surrounding pixels' intensities. It can be defined in different ways, from simple averages differences to formulas incorporating variance of the intensities. The prior forbids any overlap between the matched shapes. The sensitivity of the detector can be tuned using a minimal threshold on the data fidelity term. Using synthetic images of increasing blur, i.e., detection difficulty, we showed that, on certain images, SPADE can out-perform widely used methods in this classical task of biological image processing (Figure 2). We also show that the data fidelity term must be adapted to the nature of the images in order to get the best results. We then apply SPADE, in a real-case scenario, on con-focal fluorescence microscopy images of D. melanogaster cultured cells where cytoplasmic ribonucleoproteic granules have to be detected and characterized (Figure 3). We demonstrate that our method can be calibrated for high-throughput screening imaging study, generating a F 1 detection score of 0.76 after cross-validation using a trained biologist expertise as ground truth (data not shown). An open-source python implementation of SPADE has been released on the Python Package Index 1. Different dic-tonaries of shapes and data fidelity terms could be used for different applications." @default.
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- W2892289766 date "2018-04-04" @default.
- W2892289766 modified "2023-10-14" @default.
- W2892289766 title "SPADE: A Small Particle Detection Method Using A Dictionary Of Shapes Within The Marked Point Process Framework" @default.
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