FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892328587> ?p ?o ?g. }

• W2892328587 abstract "In the biofuel industry, cellulase plays an indispensable role in hydrolyzing cellulose into fermentable glucose. Trichoderma reesei is a popular filamentous fungus with prominent ability to produce cellulase. While classical mutagenesis and modern multiplex genome engineering are both effective ways to improve cellulase production, successful obtaining of strains with improved cellulase-producing ability requires screening a large number of strains, which is time-consuming and labor intensive.Herein, we developed a versatile method coupling expression of the red fluorescence protein (DsRed) in T. reesei and fluorescence-assisted cell sorting (FACS) of germinated spores. This method was first established by expressing DsRed intracellularly under the control of the major cellulase cbh1 promoter in T. reesei, which allowed us to rapidly isolate cellulase hyperproducers from T. reesei progenies transformed with a dedicated transcriptional activator ace3 and from an atmospheric and room temperature plasma-created mutant T. reesei library. Since intracellularly expressed DsRed was expected to isolate mutations mainly affecting cellulase transcription, this method was further improved by displaying DsRed on the T. reesei cell surface, enabling isolation of strains with beneficial genetic alterations (overexpressing hac1 and bip1) affecting regulatory stages beyond transcription. Using this method, T. reesei cellulase hyperproducers were also successfully isolated from an Agrobacterium-mediated random insertional mutant library.The coupled DsRed-FACS high-throughput screening method proved to be an effective strategy for fast isolation of T. reesei cellulase hyperproducers and could also be applied in other industrially important filamentous fungi." @default.
• W2892328587 created "2018-09-27" @default.
• W2892328587 creator A5005847066 @default.
• W2892328587 creator A5008371533 @default.
• W2892328587 creator A5032931209 @default.
• W2892328587 creator A5036217647 @default.
• W2892328587 creator A5038837730 @default.
• W2892328587 creator A5056550372 @default.
• W2892328587 creator A5066301734 @default.
• W2892328587 creator A5075043640 @default.
• W2892328587 creator A5075760116 @default.
• W2892328587 date "2018-09-24" @default.
• W2892328587 modified "2023-10-17" @default.
• W2892328587 title "A versatile system for fast screening and isolation of Trichoderma reesei cellulase hyperproducers based on DsRed and fluorescence-assisted cell sorting" @default.
• W2892328587 cites W1606862874 @default.
• W2892328587 cites W1845097650 @default.
• W2892328587 cites W1873195519 @default.
• W2892328587 cites W1953352723 @default.
• W2892328587 cites W1964324803 @default.
• W2892328587 cites W1967210731 @default.
• W2892328587 cites W1968468250 @default.
• W2892328587 cites W1970871004 @default.
• W2892328587 cites W1971439989 @default.
• W2892328587 cites W1985252740 @default.
• W2892328587 cites W1988896096 @default.
• W2892328587 cites W1989211919 @default.
• W2892328587 cites W1994803479 @default.
• W2892328587 cites W1999634015 @default.
• W2892328587 cites W2002733094 @default.
• W2892328587 cites W2016635374 @default.
• W2892328587 cites W2023767092 @default.
• W2892328587 cites W2024347957 @default.
• W2892328587 cites W2025544376 @default.
• W2892328587 cites W2026349284 @default.
• W2892328587 cites W2027219161 @default.
• W2892328587 cites W2027741551 @default.
• W2892328587 cites W2029309165 @default.
• W2892328587 cites W2033834490 @default.
• W2892328587 cites W2039530780 @default.
• W2892328587 cites W2042345705 @default.
• W2892328587 cites W2042617105 @default.
• W2892328587 cites W2043306773 @default.
• W2892328587 cites W2044622948 @default.
• W2892328587 cites W2049144064 @default.
• W2892328587 cites W2062492434 @default.
• W2892328587 cites W2067603288 @default.
• W2892328587 cites W2073265884 @default.
• W2892328587 cites W2082324597 @default.
• W2892328587 cites W2082591769 @default.
• W2892328587 cites W2090182348 @default.
• W2892328587 cites W2096947765 @default.
• W2892328587 cites W2099941694 @default.
• W2892328587 cites W2104640383 @default.
• W2892328587 cites W2119824898 @default.
• W2892328587 cites W2125648541 @default.
• W2892328587 cites W2136464092 @default.
• W2892328587 cites W2152313243 @default.
• W2892328587 cites W2156426990 @default.
• W2892328587 cites W2156911822 @default.
• W2892328587 cites W2161656422 @default.
• W2892328587 cites W2165574256 @default.
• W2892328587 cites W2168506412 @default.
• W2892328587 cites W2416079164 @default.
• W2892328587 cites W2484112271 @default.
• W2892328587 cites W2529558525 @default.
• W2892328587 cites W2577386509 @default.
• W2892328587 cites W2618962964 @default.
• W2892328587 cites W2734802359 @default.
• W2892328587 cites W2775530028 @default.
• W2892328587 cites W2803585751 @default.
• W2892328587 doi "https://doi.org/10.1186/s13068-018-1264-z" @default.
• W2892328587 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6151939" @default.
• W2892328587 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30258495" @default.
• W2892328587 hasPublicationYear "2018" @default.
• W2892328587 type Work @default.
• W2892328587 sameAs 2892328587 @default.
• W2892328587 citedByCount "34" @default.
• W2892328587 countsByYear W28923285872019 @default.
• W2892328587 countsByYear W28923285872020 @default.
• W2892328587 countsByYear W28923285872021 @default.
• W2892328587 countsByYear W28923285872022 @default.
• W2892328587 countsByYear W28923285872023 @default.
• W2892328587 crossrefType "journal-article" @default.
• W2892328587 hasAuthorship W2892328587A5005847066 @default.
• W2892328587 hasAuthorship W2892328587A5008371533 @default.
• W2892328587 hasAuthorship W2892328587A5032931209 @default.
• W2892328587 hasAuthorship W2892328587A5036217647 @default.
• W2892328587 hasAuthorship W2892328587A5038837730 @default.
• W2892328587 hasAuthorship W2892328587A5056550372 @default.
• W2892328587 hasAuthorship W2892328587A5066301734 @default.
• W2892328587 hasAuthorship W2892328587A5075043640 @default.
• W2892328587 hasAuthorship W2892328587A5075760116 @default.
• W2892328587 hasBestOaLocation W28923285871 @default.
• W2892328587 hasConcept C104317684 @default.
• W2892328587 hasConcept C141603559 @default.
• W2892328587 hasConcept C142613039 @default.
• W2892328587 hasConcept C143065580 @default.
• W2892328587 hasConcept C1491633281 @default.
• W2892328587 hasConcept C185592680 @default.
• W2892328587 hasConcept C2776125036 @default.

• next