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• W2892331119 startingPage "191" @default.
• W2892331119 abstract "Aim: Complementary medicinal techniques have gainedfocus by modern medicine, recently. Maggot Debridement Therapy is a widely-used method worldwide. It is especially recommended for chronic wounds, and has serious advantages such as low cost, easily-applicability and rare adverse effects, but its effect mechanisms remains unclear. The aim of this study is to detect components and to investigate potential antibacterial effects of whole body extract metabolites of Lucilia sericata larvae.Material anf Methods: Due to potential antibacterial effects, agar well diffusion and flowcytometry methods were used against Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Enterococcus faecalis to do evaluation on whole body extracts of previously-cloned maggots in specialized climate room. After this antibacterial effect evaluation, 2-D PAGE analysis was done for protein investigation. Results: Inhibition zones were observed for S.aureus (16mm), E.coli (22mm) and E.faecalis (14mm), but for P.aeruginosa, the extract could not provide any inhibiton zone. In flow cytometry, different killing rates were detected in different extract dilutions, and for the lowest (1/64) dilution, killing rates were 51.9%, 75%, 80% and 98.7% for P.aeruginosa, E.faecalis, E.coli and S.aureus, respectively. 2-D PAGE showed various proteins with different molercular mass (&lt;10-260kDa) and pI (3-9). Conclusion: Antibacterial effects of maggot whole body extracts on tested strains are obviously detected. Many protein spots with widely variable molecular mass and isoelectric points were observed. As a result, this antibacterial effects may be caused by these proteins, but it is necessary that these proteins must be further evaluated via mass spectrometry and protein databases." @default.
• W2892331119 created "2018-09-27" @default.
• W2892331119 creator A5004064025 @default.
• W2892331119 creator A5048755324 @default.
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• W2892331119 date "2018-09-30" @default.
• W2892331119 modified "2023-09-23" @default.
• W2892331119 title "Investigation of whole body extract metabolites of Lucilia sericata larvae and potential antibacterial effects" @default.
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• W2892331119 doi "https://doi.org/10.18663/tjcl.396308" @default.
• W2892331119 hasPublicationYear "2018" @default.
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