FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892333029> ?p ?o ?g. }

• W2892333029 endingPage "78" @default.
• W2892333029 startingPage "71" @default.
• W2892333029 abstract "Efficient extraction of proteins is a great challenge for numerous downstream proteomic analyses. During the protein extraction procedure, it is critical to maintain the conformational stability, integrity, as well as higher yield of the protein. To do so, 5-different lysis buffers of Tris and HEPES have been used as the primary buffering reagents with variable compositions at different concentrations and pH using human cancer cells. In this study, different protein lysates of human breast cancer cells T47D and MDA-MB-231 and ovarian cancer cell PA-1 were subjected to run SDS-PAGE for separation of proteins based on their molecular size, followed by Coomassie blue, silver staining, and immunoblot assays to compare the extraction yield of total cytoplasmic proteins, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and the integral membrane protein, integrin β-1. Our results revealed that Tris-based lysis buffer with 50 mM concentration, pH 7.5, is relatively the efficient and reliable protein extraction method for a wide range of MW subcellular markers, cytoplasmic GAPDH and transmembrane integrin β-1 proteins. We anticipate that this simple and cost-effective protein extraction protocol might be extremely useful across a broad range of subcellular proteins in different biologic samples." @default.
• W2892333029 created "2018-09-27" @default.
• W2892333029 creator A5020428228 @default.
• W2892333029 creator A5025313845 @default.
• W2892333029 creator A5065240149 @default.
• W2892333029 date "2018-09-01" @default.
• W2892333029 modified "2023-10-14" @default.
• W2892333029 title "Cytosolic and Transmembrane Protein Extraction Methods of Breast and Ovarian Cancer Cells: A Comparative Study" @default.
• W2892333029 cites W1894884196 @default.
• W2892333029 cites W1897193595 @default.
• W2892333029 cites W1952066406 @default.
• W2892333029 cites W1969112758 @default.
• W2892333029 cites W1969540615 @default.
• W2892333029 cites W1969908940 @default.
• W2892333029 cites W2049194781 @default.
• W2892333029 cites W2053405000 @default.
• W2892333029 cites W2054338638 @default.
• W2892333029 cites W2057013716 @default.
• W2892333029 cites W2086487142 @default.
• W2892333029 cites W2099163131 @default.
• W2892333029 cites W2130572697 @default.
• W2892333029 cites W2134562620 @default.
• W2892333029 cites W2152770371 @default.
• W2892333029 cites W2426268036 @default.
• W2892333029 cites W2553402145 @default.
• W2892333029 cites W2765362479 @default.
• W2892333029 cites W2765923272 @default.
• W2892333029 cites W2782865693 @default.
• W2892333029 cites W76832415 @default.
• W2892333029 cites W948914886 @default.
• W2892333029 doi "https://doi.org/10.7171/jbt.18-2903-002" @default.
• W2892333029 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6091320" @default.
• W2892333029 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30174558" @default.
• W2892333029 hasPublicationYear "2018" @default.
• W2892333029 type Work @default.
• W2892333029 sameAs 2892333029 @default.
• W2892333029 citedByCount "11" @default.
• W2892333029 countsByYear W28923330292018 @default.
• W2892333029 countsByYear W28923330292020 @default.
• W2892333029 countsByYear W28923330292021 @default.
• W2892333029 countsByYear W28923330292022 @default.
• W2892333029 countsByYear W28923330292023 @default.
• W2892333029 crossrefType "journal-article" @default.
• W2892333029 hasAuthorship W2892333029A5020428228 @default.
• W2892333029 hasAuthorship W2892333029A5025313845 @default.
• W2892333029 hasAuthorship W2892333029A5065240149 @default.
• W2892333029 hasBestOaLocation W28923330291 @default.
• W2892333029 hasConcept C104317684 @default.
• W2892333029 hasConcept C134792803 @default.
• W2892333029 hasConcept C153911025 @default.
• W2892333029 hasConcept C170493617 @default.
• W2892333029 hasConcept C181199279 @default.
• W2892333029 hasConcept C185592680 @default.
• W2892333029 hasConcept C190062978 @default.
• W2892333029 hasConcept C24530287 @default.
• W2892333029 hasConcept C2776317432 @default.
• W2892333029 hasConcept C2780761556 @default.
• W2892333029 hasConcept C46111723 @default.
• W2892333029 hasConcept C54355233 @default.
• W2892333029 hasConcept C55493867 @default.
• W2892333029 hasConcept C57409179 @default.
• W2892333029 hasConcept C74864618 @default.
• W2892333029 hasConcept C86803240 @default.
• W2892333029 hasConcept C8985478 @default.
• W2892333029 hasConcept C98539663 @default.
• W2892333029 hasConceptScore W2892333029C104317684 @default.
• W2892333029 hasConceptScore W2892333029C134792803 @default.
• W2892333029 hasConceptScore W2892333029C153911025 @default.
• W2892333029 hasConceptScore W2892333029C170493617 @default.
• W2892333029 hasConceptScore W2892333029C181199279 @default.
• W2892333029 hasConceptScore W2892333029C185592680 @default.
• W2892333029 hasConceptScore W2892333029C190062978 @default.
• W2892333029 hasConceptScore W2892333029C24530287 @default.
• W2892333029 hasConceptScore W2892333029C2776317432 @default.
• W2892333029 hasConceptScore W2892333029C2780761556 @default.
• W2892333029 hasConceptScore W2892333029C46111723 @default.
• W2892333029 hasConceptScore W2892333029C54355233 @default.
• W2892333029 hasConceptScore W2892333029C55493867 @default.
• W2892333029 hasConceptScore W2892333029C57409179 @default.
• W2892333029 hasConceptScore W2892333029C74864618 @default.
• W2892333029 hasConceptScore W2892333029C86803240 @default.
• W2892333029 hasConceptScore W2892333029C8985478 @default.
• W2892333029 hasConceptScore W2892333029C98539663 @default.
• W2892333029 hasIssue "3" @default.
• W2892333029 hasLocation W28923330291 @default.
• W2892333029 hasLocation W28923330292 @default.
• W2892333029 hasLocation W28923330293 @default.
• W2892333029 hasLocation W28923330294 @default.
• W2892333029 hasOpenAccess W2892333029 @default.
• W2892333029 hasPrimaryLocation W28923330291 @default.
• W2892333029 hasRelatedWork W2030492692 @default.
• W2892333029 hasRelatedWork W2126183117 @default.
• W2892333029 hasRelatedWork W2374691494 @default.
• W2892333029 hasRelatedWork W2487615444 @default.
• W2892333029 hasRelatedWork W2513218992 @default.
• W2892333029 hasRelatedWork W2544182179 @default.
• W2892333029 hasRelatedWork W2892333029 @default.
• W2892333029 hasRelatedWork W3014995365 @default.

• next