Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892444777> ?p ?o ?g. }
- W2892444777 abstract "Abstract Many bacterial pathogens hijack macrophages to egress from the port of entry to the lymphatic/blood-stream, causing dissemination of life-threatening infections. However, the underlying mechanisms are not well understood. Here, we report that Salmonella infection generates directional electric fields (EF) in the follicle-associated epithelium of mouse cecum. In vitro application of an EF, mimicking the infection-generated electric field (IGEF), induces directional migration of primary mouse macrophages to the anode, which is reversed to the cathode upon Salmonella infection. This infection-dependent directional switch is independent of the Salmonella pathogenicity island 1 (SPI-1) type III secretion system. The switch is accompanied by a reduction of sialic acids on glycosylated surface components during phagocytosis of bacteria, which is absent in macrophages challenged by microspheres. Moreover, enzymatic cleavage of terminally exposed sialic acids reduces macrophage surface negativity and severely impairs directional migration of macrophages in response to EF. Based on these findings, we propose that macrophages are attracted to the site of infection by a combination of chemotaxis and galvanotaxis; after phagocytosis of bacteria, surface electrical properties of the macrophage change, and galvanotaxis directs the cells away from the site of infection. Abbreviations CFU, colony-forming unit; Con A, Concanavalin A; EF, electric field; FAE, follicle-associated epithelium; GNL, Galanthus Nivalis lectin; IGEF, infection-generated electric field; Ji, electric current density; MAL-2, Maackia Amurensis lectin II; MLN, mesenteric lymph node; MOI, multiplicity of infection; nMFI, normalized mean fluorescence intensity; RCA-1, Ricinus Communis Agglutinin I; SNA, Sambucus Nigra lectin; S . Typhimurium, Salmonella enterica serotype Typhimurium; SPI-1, Salmonella pathogenicity island 1; PDMS, polydimethylsiloxane; TEP, trans-epithelial potential difference; TLR, Toll-like receptors; WGEF, wound-generated electric field" @default.
- W2892444777 created "2018-10-05" @default.
- W2892444777 creator A5004093071 @default.
- W2892444777 creator A5013246400 @default.
- W2892444777 creator A5021553104 @default.
- W2892444777 creator A5030729248 @default.
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- W2892444777 creator A5051538911 @default.
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- W2892444777 creator A5058025613 @default.
- W2892444777 creator A5058125672 @default.
- W2892444777 creator A5058931057 @default.
- W2892444777 creator A5073121510 @default.
- W2892444777 creator A5084956764 @default.
- W2892444777 date "2018-09-25" @default.
- W2892444777 modified "2023-09-26" @default.
- W2892444777 title "Infection-generated electric field in gut epithelium drives bidirectional migration of macrophages" @default.
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