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- W2892477113 abstract "Circadian rhythms are 24-h oscillations conserved in nearly all living organisms that allow for the anticipation of daily environmental changes. These rhythms are maintained by a molecular clock comprised of a transcriptional/translational negative feedback loop. Many of the proteins that organize this feedback loop are intrinsically disordered proteins (IDPs), which lack a fixed or ordered three-dimensional structure. Little is known about the impact of intrinsic disorder in clock proteins and this lack of comprehension is compounded by the fact that sophisticated techniques to understand the inherent nature of IDPs are only now emerging. Here, we add to that conversation by describing our novel protocol to track the conformation of a core clock protein (FREQUENCY) in a vital clock model organism (Neurospora crassa). Our protocol, CiRcadian nAtive FasT parallel proteolYsis (CRAFTY), utilizes a parallel proteolysis approach in native conditions to determine the conformational shifts in FREQUENCY over time, providing biologically relevant information and contributing to our understanding of the importance of disorder in the circadian clock." @default.
- W2892477113 created "2018-10-05" @default.
- W2892477113 creator A5008343970 @default.
- W2892477113 creator A5016869041 @default.
- W2892477113 creator A5086527018 @default.
- W2892477113 date "2018-01-01" @default.
- W2892477113 modified "2023-10-16" @default.
- W2892477113 title "Characterizing Time-of-Day Conformational Changes in the Intrinsically Disordered Proteins of the Circadian Clock" @default.
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- W2892477113 doi "https://doi.org/10.1016/bs.mie.2018.08.024" @default.
- W2892477113 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30471697" @default.
- W2892477113 hasPublicationYear "2018" @default.
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