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- W2892512205 abstract "Abstract Microbial survival in dynamic environments requires the ability to successfully respond to abrupt changes in osmolarity. The mechanosensitive channel of large conductance (MscL) is a ubiquitous channel that facilitates the survival of bacteria and archaea under severe osmotic downshock conditions by relieving excess turgor pressure in response to increased membrane tension. A prominent structural feature of MscL, the cytoplasmic C-terminal domain, has been suggested to influence channel assembly and function. In this report, we describe the X-ray crystal structure and electrophysiological properties of a C-terminal domain truncation of the Mycobacterium tuberculosis MscL ( Mt MscLΔC). A crystal structure of Mt MscLΔC solubilized in the detergent n-dodecyl-β-D-maltopyranoside reveals the pentameric, closed state-like architecture for the membrane spanning region observed in the previously solved full-length Mt MscL. Electrophysiological characterization demonstrates that Mt MscLΔC retains mechanosensitivity, but with conductance and tension sensitivity more closely resembling full length Ec MscL than Mt MscL. This study establishes that the C-terminal domain of Mt MscL is not required for oligomerization of the full-length channel, but rather influences the tension sensitivity and conductance properties of the channel. The collective picture that emerges from these data is that each MscL channel structure has characteristic features, highlighting the importance of studying multiple homologs." @default.
- W2892512205 created "2018-10-05" @default.
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- W2892512205 date "2018-10-01" @default.
- W2892512205 modified "2023-10-14" @default.
- W2892512205 title "Elucidating a role for the cytoplasmic domain in the Mycobacterium tuberculosis mechanosensitive channel of large conductance" @default.
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- W2892512205 doi "https://doi.org/10.1038/s41598-018-32536-6" @default.
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