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- W2892685910 abstract "The CRISPR/Cas9 revolution is profoundly changing the way life sciences technologies are used. Many assays now rely on engineered clonal cell lines to eliminate the overexpression of bait proteins. Control cell lines are typically nonengineered cells or engineered clones, implying a considerable risk for artifacts because of clonal variation. Genome engineering can also transform BioID, a proximity labeling method that relies on fusing a bait protein to a promiscuous biotin ligase, BirA*, resulting in the tagging of vicinal proteins. We here propose an innovative design to enable BioID for endogenous proteins wherein we introduce a T2A-BirA* module at the C-terminus of endogenous p53 by genome engineering, leading to bicistronic expression of both p53 and BirA* under control of the endogenous promoter. By targeting a Cas9-cytidine deaminase base editor to the T2A autocleavage site, we can efficiently derive an isogenic population expressing a functional p53-BirA* fusion protein. Using quantitative proteomics we show significant benefits over the classical ectopic expression of p53-BirA*, and we provide a first well-controlled view of the proximal proteins of endogenous p53 in colon carcinoma cells. This novel application for base editors expands the CRISPR/Cas9 toolbox and can be a valuable addition for synthetic biology." @default.
- W2892685910 created "2018-10-05" @default.
- W2892685910 creator A5009772719 @default.
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- W2892685910 date "2018-12-10" @default.
- W2892685910 modified "2023-09-23" @default.
- W2892685910 title "A Well-Controlled BioID Design for Endogenous Bait Proteins" @default.
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- W2892685910 cites W1838945435 @default.
- W2892685910 cites W1957507493 @default.
- W2892685910 cites W1964719993 @default.
- W2892685910 cites W1964852313 @default.
- W2892685910 cites W1967086556 @default.
- W2892685910 cites W1973163678 @default.
- W2892685910 cites W1974253337 @default.
- W2892685910 cites W1974393759 @default.
- W2892685910 cites W1977709885 @default.
- W2892685910 cites W1980496079 @default.
- W2892685910 cites W2012053348 @default.
- W2892685910 cites W2034304873 @default.
- W2892685910 cites W2038538904 @default.
- W2892685910 cites W2067196139 @default.
- W2892685910 cites W2070433620 @default.
- W2892685910 cites W2072636962 @default.
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- W2892685910 cites W2087523836 @default.
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- W2892685910 cites W2224593426 @default.
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- W2892685910 doi "https://doi.org/10.1021/acs.jproteome.8b00367" @default.
- W2892685910 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30525648" @default.
- W2892685910 hasPublicationYear "2018" @default.
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