Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892792724> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2892792724 abstract "Background: Agrobacterium mediated genetic transformation experiments were carried out in Dolichos bean Cv. (Konkan Bhushan) showing better regenerability. Methods: Three cry genes viz. cry1Aabc, cry1Fa1 and cry2Aa were used for the transformation each of which were linked to CaMV35S promoter and nptII gene under control of nos promoter and terminator. A vector system consisting of the disarmed hyper virulent Agrobacterium tumefaciens strain EHA-105 harboring pBinAR or BinBt3 was used. Mature embryo axis with single cotyledon was used as explant. Kanamycin as well as PCR screening was carried out to assess the transformation frequency. Progeny analysis using PCR was also carried out to assess the transgene segregation and stable transformation.Result: Kanamycin concentration of 500 mg/l was found as optimum for selection of a transgenic turning leaf blades albino. Among five methods of colonization used, the method employing mild injury to explant with dipping in Agrobacterium culture for 20 minutes followed by co-cultivation for 48 hours, cefotaxime washing and sowing in soil resulted in maximum survival (74.80%) associated with maximum transformation frequency through PCR analysis (2.13%). Among three cry genes, the gene cry2Aa was found the most effective in transforming Dolichos bean. The progeny analysis of transformants has shown the 3:1 mendelian segregation ratio confirming stable transformation of transgene." @default.
- W2892792724 created "2018-10-05" @default.
- W2892792724 creator A5016278111 @default.
- W2892792724 creator A5032352488 @default.
- W2892792724 creator A5053050804 @default.
- W2892792724 creator A5058421460 @default.
- W2892792724 creator A5067877583 @default.
- W2892792724 creator A5084281058 @default.
- W2892792724 creator A5089719299 @default.
- W2892792724 date "2021-05-25" @default.
- W2892792724 modified "2023-09-27" @default.
- W2892792724 title "Genetic Transformation for Pod Borer Resistance in Dolichos Bean [Lablab purpureus (L.) Sweet]" @default.
- W2892792724 doi "https://doi.org/10.18805/lr-4471" @default.
- W2892792724 hasPublicationYear "2021" @default.
- W2892792724 type Work @default.
- W2892792724 sameAs 2892792724 @default.
- W2892792724 citedByCount "0" @default.
- W2892792724 crossrefType "journal-article" @default.
- W2892792724 hasAuthorship W2892792724A5016278111 @default.
- W2892792724 hasAuthorship W2892792724A5032352488 @default.
- W2892792724 hasAuthorship W2892792724A5053050804 @default.
- W2892792724 hasAuthorship W2892792724A5058421460 @default.
- W2892792724 hasAuthorship W2892792724A5067877583 @default.
- W2892792724 hasAuthorship W2892792724A5084281058 @default.
- W2892792724 hasAuthorship W2892792724A5089719299 @default.
- W2892792724 hasConcept C102230213 @default.
- W2892792724 hasConcept C104317684 @default.
- W2892792724 hasConcept C122172344 @default.
- W2892792724 hasConcept C144027150 @default.
- W2892792724 hasConcept C204241405 @default.
- W2892792724 hasConcept C2776162087 @default.
- W2892792724 hasConcept C2776632002 @default.
- W2892792724 hasConcept C2777948272 @default.
- W2892792724 hasConcept C2779656422 @default.
- W2892792724 hasConcept C2780920689 @default.
- W2892792724 hasConcept C2909510519 @default.
- W2892792724 hasConcept C54355233 @default.
- W2892792724 hasConcept C59822182 @default.
- W2892792724 hasConcept C86803240 @default.
- W2892792724 hasConceptScore W2892792724C102230213 @default.
- W2892792724 hasConceptScore W2892792724C104317684 @default.
- W2892792724 hasConceptScore W2892792724C122172344 @default.
- W2892792724 hasConceptScore W2892792724C144027150 @default.
- W2892792724 hasConceptScore W2892792724C204241405 @default.
- W2892792724 hasConceptScore W2892792724C2776162087 @default.
- W2892792724 hasConceptScore W2892792724C2776632002 @default.
- W2892792724 hasConceptScore W2892792724C2777948272 @default.
- W2892792724 hasConceptScore W2892792724C2779656422 @default.
- W2892792724 hasConceptScore W2892792724C2780920689 @default.
- W2892792724 hasConceptScore W2892792724C2909510519 @default.
- W2892792724 hasConceptScore W2892792724C54355233 @default.
- W2892792724 hasConceptScore W2892792724C59822182 @default.
- W2892792724 hasConceptScore W2892792724C86803240 @default.
- W2892792724 hasLocation W28927927241 @default.
- W2892792724 hasOpenAccess W2892792724 @default.
- W2892792724 hasPrimaryLocation W28927927241 @default.
- W2892792724 hasRelatedWork W11852517 @default.
- W2892792724 hasRelatedWork W20277378 @default.
- W2892792724 hasRelatedWork W26960367 @default.
- W2892792724 hasRelatedWork W31890670 @default.
- W2892792724 hasRelatedWork W3725137 @default.
- W2892792724 hasRelatedWork W38583529 @default.
- W2892792724 hasRelatedWork W5863606 @default.
- W2892792724 hasRelatedWork W6014467 @default.
- W2892792724 hasRelatedWork W7559939 @default.
- W2892792724 hasRelatedWork W769668 @default.
- W2892792724 isParatext "false" @default.
- W2892792724 isRetracted "false" @default.
- W2892792724 magId "2892792724" @default.
- W2892792724 workType "article" @default.