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• W2892883663 abstract "Zebrafish larvae are transparent and the entire gastrointestinal (GI) tract is easily visualized. Application of a new image analysis technique is reported in this issue of Neurogastroenterology and Motility (Neurogastroenterol Motil., 2018, volume 30, e13351). The technique quantifies movement in images collected in a timed sequence, and characterizes smooth muscle contractions based on contraction distance and frequency. The technique also reports the contraction amplitude, or the distance moved. This technique, and current spatiotemporal mapping techniques, are essential tools enabling characterization of GI motility patterns in intact physiological settings. Advances and development of transgenic zebrafish that lack pigmentation, with calcium reporters expressed in specific cell types, or with inactivation of specific genes contribute to our understanding of the generation, and regulation of GI motility at the molecular, cellular, and systemic level. Finally, development of chambers that immobilize zebrafish larvae for long-duration imaging will contribute to our technique toolbox, and will provide an increased experimental throughput." @default.
• W2892883663 created "2018-10-05" @default.
• W2892883663 creator A5028324593 @default.
• W2892883663 date "2018-09-01" @default.
• W2892883663 modified "2023-10-03" @default.
• W2892883663 title "Improved Imaging of Zebrafish Motility" @default.
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• W2892883663 doi "https://doi.org/10.1111/nmo.13435" @default.
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