FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892923998> ?p ?o ?g. }

• W2892923998 endingPage "297" @default.
• W2892923998 startingPage "291" @default.
• W2892923998 abstract "The causative agent of tuberculosis is the bacterium Mycobacterium tuberculosis. It is the second leading cause of death worldwide. The development of resistance associated with this bacterium is of great concern, and the ability of the bacteria to form protective biofilms and persist for longer periods of time needs to be considered as a possible target. Plants have been considered as an additive to current treatments as an adjuvant, to aid the body in fighting the disease and as a source for new drug candidates to aid in the development of resistance of mycobacteria to available drugs. Twenty plants were selected based on their traditional usage against tuberculosis and related symptoms. The antimycobacterial activity of the ethanolic extracts from different genera and variable plant parts were evaluated using the microplate Alamar Blue assay. A biofilm formation inhibition assay was adapted and used to determine the inhibitory activity against Mycobacterium smegmatis biofilm formation. The cytotoxic effect of the plants was determined on U937 human macrophage cells using the XTT (2, 3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) cell proliferation kit. ADTNB-coupled Glutathione/Mycothiol disulfide reductase assay was used to determine the inhibitory activity against the human and bacterial reductases. Of the 20 plant extracts tested, it was found that Salvia africana-lutea, Ficus sur and Sphedamnocarpus pruriens showed moderate antimycobacterial activity with minimum inhibitory concentration values of 31.25, 62.5 and 62.5 μg/ml, respectively. Sphedamnocarpus pruriens (BF SI 1.00) and S.africana-lutea (BF SI 0.33) showed potential biofilm formation inhibition at concentrations, 62.2 and 95.8 μg/ml, respectively when compared to Ciprofloxacin (BF SI 0.32), which inhibited at a concentration of 1.9 μg/ml. Most of the plant extracts showed low to moderate cellular toxicity except Withania somnifera that showed a higher toxicity with an IC50 of 6.8 μg/ml (Actinomycin D0.0009 μg/ml). F.sur showed the highest selectivity index of 3.36. During mechanistic studies, S.africana-lutea showed inhibitory activity against Mycothiol disulfide reductase (Mtr) exhibiting an inhibitory concentration (IC50) of 102.3 μg/ml and showed lower inhibitory activity against Glutathione reductase (Gtr) with an IC50 of 224.1 μg/ml. This study indicates that South African plant extracts traditionally used to treat tuberculosis-related symptoms have reasonable antimycobacterial, antibiofilm and enzyme inhibitory activity with relatively low toxicity." @default.
• W2892923998 created "2018-10-05" @default.
• W2892923998 creator A5006032909 @default.
• W2892923998 creator A5016596289 @default.
• W2892923998 creator A5049308185 @default.
• W2892923998 creator A5069664398 @default.
• W2892923998 date "2019-01-01" @default.
• W2892923998 modified "2023-09-26" @default.
• W2892923998 title "Inhibition of mycothione disulphide reductase and mycobacterial biofilm by selected South African plants" @default.
• W2892923998 cites W1857912440 @default.
• W2892923998 cites W1964366874 @default.
• W2892923998 cites W1966057681 @default.
• W2892923998 cites W1981444004 @default.
• W2892923998 cites W1982133789 @default.
• W2892923998 cites W1982415698 @default.
• W2892923998 cites W1985871973 @default.
• W2892923998 cites W1987886315 @default.
• W2892923998 cites W1989991378 @default.
• W2892923998 cites W1990068847 @default.
• W2892923998 cites W1992840257 @default.
• W2892923998 cites W1992933299 @default.
• W2892923998 cites W1997221487 @default.
• W2892923998 cites W2000377779 @default.
• W2892923998 cites W2000821395 @default.
• W2892923998 cites W2007075117 @default.
• W2892923998 cites W2009185448 @default.
• W2892923998 cites W2016460939 @default.
• W2892923998 cites W2019432687 @default.
• W2892923998 cites W2023446287 @default.
• W2892923998 cites W2042268077 @default.
• W2892923998 cites W2042501392 @default.
• W2892923998 cites W2049860537 @default.
• W2892923998 cites W2065456568 @default.
• W2892923998 cites W2066951899 @default.
• W2892923998 cites W2068175557 @default.
• W2892923998 cites W2070845766 @default.
• W2892923998 cites W2073312672 @default.
• W2892923998 cites W2077351842 @default.
• W2892923998 cites W2081372892 @default.
• W2892923998 cites W2086644936 @default.
• W2892923998 cites W2099655800 @default.
• W2892923998 cites W2111393577 @default.
• W2892923998 cites W2134736970 @default.
• W2892923998 cites W2141388665 @default.
• W2892923998 cites W2144120153 @default.
• W2892923998 cites W2157525511 @default.
• W2892923998 cites W2160272913 @default.
• W2892923998 cites W2160787060 @default.
• W2892923998 cites W2535198814 @default.
• W2892923998 cites W2566316489 @default.
• W2892923998 cites W2606849962 @default.
• W2892923998 cites W4211085923 @default.
• W2892923998 cites W4243315380 @default.
• W2892923998 doi "https://doi.org/10.1016/j.sajb.2018.09.015" @default.
• W2892923998 hasPublicationYear "2019" @default.
• W2892923998 type Work @default.
• W2892923998 sameAs 2892923998 @default.
• W2892923998 citedByCount "14" @default.
• W2892923998 countsByYear W28929239982019 @default.
• W2892923998 countsByYear W28929239982020 @default.
• W2892923998 countsByYear W28929239982021 @default.
• W2892923998 countsByYear W28929239982022 @default.
• W2892923998 countsByYear W28929239982023 @default.
• W2892923998 crossrefType "journal-article" @default.
• W2892923998 hasAuthorship W2892923998A5006032909 @default.
• W2892923998 hasAuthorship W2892923998A5016596289 @default.
• W2892923998 hasAuthorship W2892923998A5049308185 @default.
• W2892923998 hasAuthorship W2892923998A5069664398 @default.
• W2892923998 hasBestOaLocation W28929239981 @default.
• W2892923998 hasConcept C142724271 @default.
• W2892923998 hasConcept C176947019 @default.
• W2892923998 hasConcept C2776967927 @default.
• W2892923998 hasConcept C2777204207 @default.
• W2892923998 hasConcept C2777975735 @default.
• W2892923998 hasConcept C2779685592 @default.
• W2892923998 hasConcept C2779707156 @default.
• W2892923998 hasConcept C2780255822 @default.
• W2892923998 hasConcept C2781069245 @default.
• W2892923998 hasConcept C4937899 @default.
• W2892923998 hasConcept C523546767 @default.
• W2892923998 hasConcept C54355233 @default.
• W2892923998 hasConcept C55493867 @default.
• W2892923998 hasConcept C556039675 @default.
• W2892923998 hasConcept C58123911 @default.
• W2892923998 hasConcept C59822182 @default.
• W2892923998 hasConcept C62112901 @default.
• W2892923998 hasConcept C71924100 @default.
• W2892923998 hasConcept C86803240 @default.
• W2892923998 hasConcept C89423630 @default.
• W2892923998 hasConceptScore W2892923998C142724271 @default.
• W2892923998 hasConceptScore W2892923998C176947019 @default.
• W2892923998 hasConceptScore W2892923998C2776967927 @default.
• W2892923998 hasConceptScore W2892923998C2777204207 @default.
• W2892923998 hasConceptScore W2892923998C2777975735 @default.
• W2892923998 hasConceptScore W2892923998C2779685592 @default.
• W2892923998 hasConceptScore W2892923998C2779707156 @default.
• W2892923998 hasConceptScore W2892923998C2780255822 @default.
• W2892923998 hasConceptScore W2892923998C2781069245 @default.

• next