FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892933698> ?p ?o ?g. }

• W2892933698 endingPage "48" @default.
• W2892933698 startingPage "40" @default.
• W2892933698 abstract "Determining the precursor/product ion pair and optimal collision energy are the critical steps for developing a multiple reaction monitoring (MRM) assay using triple quadruple mass spectrometer for protein quantitation. In this study, a platform consisting of stable isotope dimethyl labeling coupled with triple-quadruple mass spectrometer was used to quantify the protein components of the influenza vaccines. Dimethyl labeling of both the peptide N-termini and the ϵ-amino group of lysine residues was achieved by reductive amination using formaldehyde and sodium cyanoborohydrate. Dimethylated peptides are known to exhibit dominant a1 ions under gas phase fragmentation in a mass spectrometer. These a1 ions can be predicted from the peptide N-terminal amino acids, and their signals do not vary significantly across a wide range of collision energies, which facilitates the determination of MRM transition settings for multiple protein targets. The intrinsic a1 ions provide sensitivity for acquiring MRM peaks that is superior to that of the typical b/y ions used for native peptides, and they also provided good linearity (R2 ≥ 0.99) at the detected concentration range for each peptide. These features allow for the simultaneous quantification of hemagglutinin and neuraminidase in vaccines derived from either embryo eggs or cell cultivation. Moreover, the low abundant ovalbumin residue originated from the manufacturing process can also be determined. The results demonstrate that the stable isotope dimethyl labeling coupled with MRM Mass spectrometry screening of a1 ions (i.e., SIDa-MS) can be used as a high-throughput platform for multiple protein quantification of vaccine products." @default.
• W2892933698 created "2018-10-05" @default.
• W2892933698 creator A5001769670 @default.
• W2892933698 creator A5018799038 @default.
• W2892933698 creator A5052193448 @default.
• W2892933698 creator A5055455485 @default.
• W2892933698 creator A5060066201 @default.
• W2892933698 creator A5061417488 @default.
• W2892933698 creator A5087873961 @default.
• W2892933698 date "2019-01-01" @default.
• W2892933698 modified "2023-10-14" @default.
• W2892933698 title "Application of stable isotope dimethyl labeling for MRM based absolute antigen quantification of influenza vaccine" @default.
• W2892933698 cites W1481106099 @default.
• W2892933698 cites W1900060674 @default.
• W2892933698 cites W1953786867 @default.
• W2892933698 cites W1961237441 @default.
• W2892933698 cites W1972394235 @default.
• W2892933698 cites W1984855202 @default.
• W2892933698 cites W1987429263 @default.
• W2892933698 cites W1991683095 @default.
• W2892933698 cites W2001636778 @default.
• W2892933698 cites W2002778277 @default.
• W2892933698 cites W2017795981 @default.
• W2892933698 cites W2023049750 @default.
• W2892933698 cites W2023260449 @default.
• W2892933698 cites W2036741271 @default.
• W2892933698 cites W2049828693 @default.
• W2892933698 cites W2085528857 @default.
• W2892933698 cites W2087298314 @default.
• W2892933698 cites W2092014341 @default.
• W2892933698 cites W2092102543 @default.
• W2892933698 cites W2092442619 @default.
• W2892933698 cites W2094823208 @default.
• W2892933698 cites W2104764521 @default.
• W2892933698 cites W2109257248 @default.
• W2892933698 cites W2115722053 @default.
• W2892933698 cites W2140212776 @default.
• W2892933698 cites W2141091417 @default.
• W2892933698 cites W2152665645 @default.
• W2892933698 cites W2741095919 @default.
• W2892933698 cites W4210606555 @default.
• W2892933698 doi "https://doi.org/10.1016/j.jchromb.2018.09.020" @default.
• W2892933698 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30428430" @default.
• W2892933698 hasPublicationYear "2019" @default.
• W2892933698 type Work @default.
• W2892933698 sameAs 2892933698 @default.
• W2892933698 citedByCount "3" @default.
• W2892933698 countsByYear W28929336982020 @default.
• W2892933698 countsByYear W28929336982023 @default.
• W2892933698 crossrefType "journal-article" @default.
• W2892933698 hasAuthorship W2892933698A5001769670 @default.
• W2892933698 hasAuthorship W2892933698A5018799038 @default.
• W2892933698 hasAuthorship W2892933698A5052193448 @default.
• W2892933698 hasAuthorship W2892933698A5055455485 @default.
• W2892933698 hasAuthorship W2892933698A5060066201 @default.
• W2892933698 hasAuthorship W2892933698A5061417488 @default.
• W2892933698 hasAuthorship W2892933698A5087873961 @default.
• W2892933698 hasConcept C104317684 @default.
• W2892933698 hasConcept C111919701 @default.
• W2892933698 hasConcept C161790260 @default.
• W2892933698 hasConcept C162356407 @default.
• W2892933698 hasConcept C185592680 @default.
• W2892933698 hasConcept C191015642 @default.
• W2892933698 hasConcept C2779281246 @default.
• W2892933698 hasConcept C2779938440 @default.
• W2892933698 hasConcept C31827203 @default.
• W2892933698 hasConcept C41008148 @default.
• W2892933698 hasConcept C43617362 @default.
• W2892933698 hasConcept C46111723 @default.
• W2892933698 hasConcept C55493867 @default.
• W2892933698 hasConcept C68289359 @default.
• W2892933698 hasConcept C71043704 @default.
• W2892933698 hasConcept C80311884 @default.
• W2892933698 hasConceptScore W2892933698C104317684 @default.
• W2892933698 hasConceptScore W2892933698C111919701 @default.
• W2892933698 hasConceptScore W2892933698C161790260 @default.
• W2892933698 hasConceptScore W2892933698C162356407 @default.
• W2892933698 hasConceptScore W2892933698C185592680 @default.
• W2892933698 hasConceptScore W2892933698C191015642 @default.
• W2892933698 hasConceptScore W2892933698C2779281246 @default.
• W2892933698 hasConceptScore W2892933698C2779938440 @default.
• W2892933698 hasConceptScore W2892933698C31827203 @default.
• W2892933698 hasConceptScore W2892933698C41008148 @default.
• W2892933698 hasConceptScore W2892933698C43617362 @default.
• W2892933698 hasConceptScore W2892933698C46111723 @default.
• W2892933698 hasConceptScore W2892933698C55493867 @default.
• W2892933698 hasConceptScore W2892933698C68289359 @default.
• W2892933698 hasConceptScore W2892933698C71043704 @default.
• W2892933698 hasConceptScore W2892933698C80311884 @default.
• W2892933698 hasFunder F4320322108 @default.
• W2892933698 hasFunder F4320322845 @default.
• W2892933698 hasLocation W28929336981 @default.
• W2892933698 hasLocation W28929336982 @default.
• W2892933698 hasOpenAccess W2892933698 @default.
• W2892933698 hasPrimaryLocation W28929336981 @default.
• W2892933698 hasRelatedWork W2014605936 @default.
• W2892933698 hasRelatedWork W2024923641 @default.
• W2892933698 hasRelatedWork W2035971591 @default.

• next