FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892938240> ?p ?o ?g. }

• W2892938240 abstract "ABSTRACT Staphylococcus aureus infections represent the major cause of titanium based-orthopaedic implant failure. Current treatments for S. aureus infections involve the systemic delivery of antibiotics and additional surgeries, increasing health-care costs and affecting patient’s quality of life. As a step toward the development of new strategies that can prevent these infections, we build upon previous work demonstrating that the colonization of catheters by the fungal pathogen Candida albicans can be prevented by coating them with thin polymer multilayers composed of chitosan (CH) and hyaluronic acid (HA) designed to release a β-amino acid-based peptidomimetic of antimicrobial peptides (AMPs). We demonstrate here that this β-peptide is also potent against S. aureus (MIC = 4 µg/mL) and characterize its selectivity toward S. aureus biofilms. We demonstrate further that β-peptide-containing CH/HA thin-films can be fabricated on the surfaces of rough planar titanium substrates in ways that allow mammalian cell attachment and permit the long-term release of β-peptide. β-Peptide loading on CH/HA thin-films was then adjusted to achieve release of β-peptide quantities that selectively prevent S. aureus biofilms on titanium substrates in vitro for up to 24 days and remained antimicrobial after being challenged sequentially five times with S. aureus inocula, while causing no significant MC3T3-E1 preosteoblast cytotoxicity compared to uncoated and film-coated controls lacking β-peptide. We conclude that these β-peptide-containing films offer a novel and promising localized delivery approach for preventing orthopaedic implant infections. The facile fabrication and loading of β-peptide-containing films reported here provides opportunities for coating other medical devices prone to biofilm-associated infections. STATEMENT OF SIGNIFICANCE Titanium (Ti) and its alloys are used widely in internal fixation devices due to their mechanical strength and long-term biocompatibility. However, these devices are susceptible to bacterial colonization and the subsequent formation of biofilms. Here we report a chitosan and hyaluronic acid polyelectrolyte multilayer-based approach for the localized delivery of helical, cationic, globally amphiphilic β-peptide mimetics of antimicrobial peptides to inhibit S. aureus colonization and biofilm formation. Our results reveal that controlled release of this β-peptide can selectively kill S. aureus cells without exhibiting toxicity toward MC3T3-E1 preosteoblast cells. Further development of this polymer-based coating could result in new strategies for preventing orthopaedic implant-related infections, improving outcomes of these titanium implants." @default.
• W2892938240 created "2018-10-05" @default.
• W2892938240 creator A5014585172 @default.
• W2892938240 creator A5021033521 @default.
• W2892938240 creator A5056112122 @default.
• W2892938240 creator A5062284472 @default.
• W2892938240 creator A5072436102 @default.
• W2892938240 date "2018-09-30" @default.
• W2892938240 modified "2023-09-26" @default.
• W2892938240 title "Preventing S. aureus biofilm formation on titanium surfaces by the release of antimicrobial β-peptides from polyelectrolyte multilayers" @default.
• W2892938240 cites W1498038978 @default.
• W2892938240 cites W1501359805 @default.
• W2892938240 cites W1590554107 @default.
• W2892938240 cites W1602877696 @default.
• W2892938240 cites W1947538072 @default.
• W2892938240 cites W1963878748 @default.
• W2892938240 cites W1967858969 @default.
• W2892938240 cites W1969556666 @default.
• W2892938240 cites W1973714798 @default.
• W2892938240 cites W1975128800 @default.
• W2892938240 cites W1975313075 @default.
• W2892938240 cites W1979491885 @default.
• W2892938240 cites W1983255179 @default.
• W2892938240 cites W1988871507 @default.
• W2892938240 cites W1989998611 @default.
• W2892938240 cites W1991620243 @default.
• W2892938240 cites W1996689705 @default.
• W2892938240 cites W1997152791 @default.
• W2892938240 cites W1997520863 @default.
• W2892938240 cites W2000238227 @default.
• W2892938240 cites W2000643795 @default.
• W2892938240 cites W2003335879 @default.
• W2892938240 cites W2008158617 @default.
• W2892938240 cites W2013161496 @default.
• W2892938240 cites W2022199852 @default.
• W2892938240 cites W2032951551 @default.
• W2892938240 cites W2036628662 @default.
• W2892938240 cites W2038581855 @default.
• W2892938240 cites W2042654987 @default.
• W2892938240 cites W2044504436 @default.
• W2892938240 cites W2045131152 @default.
• W2892938240 cites W2045856259 @default.
• W2892938240 cites W2052042148 @default.
• W2892938240 cites W2053988366 @default.
• W2892938240 cites W2056145482 @default.
• W2892938240 cites W2069593470 @default.
• W2892938240 cites W2081277508 @default.
• W2892938240 cites W2083183071 @default.
• W2892938240 cites W2091743069 @default.
• W2892938240 cites W2095237953 @default.
• W2892938240 cites W2103743650 @default.
• W2892938240 cites W2105116823 @default.
• W2892938240 cites W2107830936 @default.
• W2892938240 cites W2108152814 @default.
• W2892938240 cites W2109163655 @default.
• W2892938240 cites W2115448390 @default.
• W2892938240 cites W2119682743 @default.
• W2892938240 cites W2124384123 @default.
• W2892938240 cites W2128672939 @default.
• W2892938240 cites W2132287875 @default.
• W2892938240 cites W2136423918 @default.
• W2892938240 cites W2152142388 @default.
• W2892938240 cites W2168505406 @default.
• W2892938240 cites W2265531934 @default.
• W2892938240 cites W2292608623 @default.
• W2892938240 cites W2317356664 @default.
• W2892938240 cites W2323677389 @default.
• W2892938240 cites W2342369213 @default.
• W2892938240 cites W2465620007 @default.
• W2892938240 cites W2473736819 @default.
• W2892938240 cites W2592917363 @default.
• W2892938240 cites W2730334649 @default.
• W2892938240 cites W2753231769 @default.
• W2892938240 doi "https://doi.org/10.1101/431205" @default.
• W2892938240 hasPublicationYear "2018" @default.
• W2892938240 type Work @default.
• W2892938240 sameAs 2892938240 @default.
• W2892938240 citedByCount "0" @default.
• W2892938240 crossrefType "posted-content" @default.
• W2892938240 hasAuthorship W2892938240A5014585172 @default.
• W2892938240 hasAuthorship W2892938240A5021033521 @default.
• W2892938240 hasAuthorship W2892938240A5056112122 @default.
• W2892938240 hasAuthorship W2892938240A5062284472 @default.
• W2892938240 hasAuthorship W2892938240A5072436102 @default.
• W2892938240 hasBestOaLocation W28929382401 @default.
• W2892938240 hasConcept C109316439 @default.
• W2892938240 hasConcept C178790620 @default.
• W2892938240 hasConcept C185592680 @default.
• W2892938240 hasConcept C192562407 @default.
• W2892938240 hasConcept C202751555 @default.
• W2892938240 hasConcept C2777529286 @default.
• W2892938240 hasConcept C2779281246 @default.
• W2892938240 hasConcept C2779489039 @default.
• W2892938240 hasConcept C2780917455 @default.
• W2892938240 hasConcept C4937899 @default.
• W2892938240 hasConcept C501593827 @default.
• W2892938240 hasConcept C506065880 @default.
• W2892938240 hasConcept C523546767 @default.
• W2892938240 hasConcept C540938839 @default.
• W2892938240 hasConcept C54355233 @default.

• next