FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2892999997> ?p ?o ?g. }

• W2892999997 abstract "Efforts to stimulate economic independence while remaining environmentally conscious have motivated researchers to replace non-renewable resources through the engineering of suitable microbes. The efficient generation of polymer precursors, such as maleic acid, through engineered microbial strains will contribute to this aim. This work proposed an engineered strain of Escherichia coli to produce the non-native dicarboxylic acid, maleic acid. The strategy included the design of a synthetic metabolic pathway to overproduce fumaric acid, which would serve as a substrate for the heterologously expressed maleate isomerase. The modifications to the E. coli strain included 6 knockouts, that resulted in a 10-fold increase in fumaric acid production (7.46mg/l vs. 0.78mg/l). The kinetic characteristics of maleate isomerases had not previously been explored in great detail, which prompted the inclusion of a screening of putative isomerases for optimal activity in the synthetic pathway. A library of 55 maleate isomerase candidates was generated from published literature and bioinformatics databases. Activity of the candidates was screened; the favoured forward reaction (maleic acid to fumaric acid) was observed in 23 variants, and the desired reverse reaction was observed in 13 variants. Maleic acid was successfully produced in vivo at a titre of about 100μg/l, which will require significant improvement before industrial implementation is possible. This is however, to our knowledge, the first time a host has been engineered to produce maleic acid using maleate isomerases. Future optimization of this production strain will contribute to a reduced dependence on petroleum as a resource for the polymer precursor, maleic acid." @default.
• W2892999997 created "2018-10-05" @default.
• W2892999997 creator A5043212957 @default.
• W2892999997 date "2017-11-28" @default.
• W2892999997 modified "2023-09-23" @default.
• W2892999997 title "Screening of a library of putative maleate isomerases for the engineering of a synthetic maleic acid-producing Escherichia coli strain." @default.
• W2892999997 cites W1579490863 @default.
• W2892999997 cites W163747836 @default.
• W2892999997 cites W1660421813 @default.
• W2892999997 cites W1963834318 @default.
• W2892999997 cites W1980240898 @default.
• W2892999997 cites W1980453473 @default.
• W2892999997 cites W1980789643 @default.
• W2892999997 cites W1985284376 @default.
• W2892999997 cites W1985849893 @default.
• W2892999997 cites W1985975348 @default.
• W2892999997 cites W1999640817 @default.
• W2892999997 cites W2001791151 @default.
• W2892999997 cites W2002533469 @default.
• W2892999997 cites W2003360218 @default.
• W2892999997 cites W2005005020 @default.
• W2892999997 cites W2016943817 @default.
• W2892999997 cites W2017862866 @default.
• W2892999997 cites W2019454936 @default.
• W2892999997 cites W2025857073 @default.
• W2892999997 cites W2041031323 @default.
• W2892999997 cites W2042101437 @default.
• W2892999997 cites W2044155398 @default.
• W2892999997 cites W2045641994 @default.
• W2892999997 cites W2045780029 @default.
• W2892999997 cites W2046918470 @default.
• W2892999997 cites W2060024445 @default.
• W2892999997 cites W2062275861 @default.
• W2892999997 cites W2073783833 @default.
• W2892999997 cites W2085881499 @default.
• W2892999997 cites W2095110809 @default.
• W2892999997 cites W2095139199 @default.
• W2892999997 cites W2095485829 @default.
• W2892999997 cites W2103385859 @default.
• W2892999997 cites W2106861615 @default.
• W2892999997 cites W2110612352 @default.
• W2892999997 cites W2111967267 @default.
• W2892999997 cites W2113497222 @default.
• W2892999997 cites W2123368500 @default.
• W2892999997 cites W2130591372 @default.
• W2892999997 cites W2133476699 @default.
• W2892999997 cites W2136072677 @default.
• W2892999997 cites W2137121087 @default.
• W2892999997 cites W2140670503 @default.
• W2892999997 cites W2140831068 @default.
• W2892999997 cites W2144678314 @default.
• W2892999997 cites W2148771150 @default.
• W2892999997 cites W2151618686 @default.
• W2892999997 cites W2152207030 @default.
• W2892999997 cites W2156195031 @default.
• W2892999997 cites W2158714788 @default.
• W2892999997 cites W2159482845 @default.
• W2892999997 cites W2159559614 @default.
• W2892999997 cites W2162115836 @default.
• W2892999997 cites W2165612309 @default.
• W2892999997 cites W2224056471 @default.
• W2892999997 cites W2293410513 @default.
• W2892999997 cites W2372200462 @default.
• W2892999997 cites W2399322857 @default.
• W2892999997 cites W2492827454 @default.
• W2892999997 cites W2560707317 @default.
• W2892999997 cites W2573984017 @default.
• W2892999997 cites W2604888580 @default.
• W2892999997 cites W2605323227 @default.
• W2892999997 cites W2617140651 @default.
• W2892999997 cites W2618738256 @default.
• W2892999997 cites W619602509 @default.
• W2892999997 hasPublicationYear "2017" @default.
• W2892999997 type Work @default.
• W2892999997 sameAs 2892999997 @default.
• W2892999997 citedByCount "0" @default.
• W2892999997 crossrefType "dissertation" @default.
• W2892999997 hasAuthorship W2892999997A5043212957 @default.
• W2892999997 hasConcept C104317684 @default.
• W2892999997 hasConcept C105702510 @default.
• W2892999997 hasConcept C15920480 @default.
• W2892999997 hasConcept C178790620 @default.
• W2892999997 hasConcept C181199279 @default.
• W2892999997 hasConcept C185592680 @default.
• W2892999997 hasConcept C2776163829 @default.
• W2892999997 hasConcept C2778022156 @default.
• W2892999997 hasConcept C2779146789 @default.
• W2892999997 hasConcept C2781206178 @default.
• W2892999997 hasConcept C2781264208 @default.
• W2892999997 hasConcept C521977710 @default.
• W2892999997 hasConcept C547475151 @default.
• W2892999997 hasConcept C55493867 @default.
• W2892999997 hasConcept C6350086 @default.
• W2892999997 hasConcept C86803240 @default.
• W2892999997 hasConceptScore W2892999997C104317684 @default.
• W2892999997 hasConceptScore W2892999997C105702510 @default.
• W2892999997 hasConceptScore W2892999997C15920480 @default.
• W2892999997 hasConceptScore W2892999997C178790620 @default.
• W2892999997 hasConceptScore W2892999997C181199279 @default.
• W2892999997 hasConceptScore W2892999997C185592680 @default.

• next