FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893003330> ?p ?o ?g. }

• W2893003330 abstract "CD99/MIC2 gene product is a heavily glycosylated transmembrane protein which plays a major role in homotypic cell adhesion, apoptosis of double positive T cells and vesicular protein trafficking. It is over expressed in various cancers and has been considered as an ideal therapeutic target. The present study focused at developing monoclonal antibodies against the extracellular domain (ECD) of CD99 using hybridoma technology.In order to generate monoclonal antibodies, the recombinant ECD of CD99 was used for immunizing the mice. Resulting hybridomas were screened through indirect ELISA. Clones which gave high absorbance values were sub cloned by limiting dilution followed by isotype determination, IP, WB and FACS. The monoclonal antibody 547F2 4F12 was purified from culture supernatant using FPLC and further screened using IF. Finally, the antibodies were validated for specificity using siRNA knock-down.We were able to establish stable hybridoma clones secreting CD99 antibodies. The antibodies reacted with both the recombinant ECD as well as the wild type CD99 and their isotype's were determined as IgM.Based on these results, we propose that the purified monoclonal antibody 547F2 4F12 could be possibly used for targeting tumors which over express CD99." @default.
• W2893003330 created "2018-10-05" @default.
• W2893003330 creator A5016114675 @default.
• W2893003330 creator A5025147028 @default.
• W2893003330 creator A5034791601 @default.
• W2893003330 creator A5047400431 @default.
• W2893003330 creator A5083143645 @default.
• W2893003330 date "2018-12-03" @default.
• W2893003330 modified "2023-09-24" @default.
• W2893003330 title "Production and characterization of monoclonal antibodies against recombinant extracellular domain of CD99" @default.
• W2893003330 cites W1752653228 @default.
• W2893003330 cites W1899517018 @default.
• W2893003330 cites W1963957597 @default.
• W2893003330 cites W1987110492 @default.
• W2893003330 cites W1987920176 @default.
• W2893003330 cites W1994265986 @default.
• W2893003330 cites W1994911302 @default.
• W2893003330 cites W1995191795 @default.
• W2893003330 cites W1996384037 @default.
• W2893003330 cites W1998818064 @default.
• W2893003330 cites W2005737029 @default.
• W2893003330 cites W2007957539 @default.
• W2893003330 cites W2025092357 @default.
• W2893003330 cites W2025329768 @default.
• W2893003330 cites W2030372367 @default.
• W2893003330 cites W2033392236 @default.
• W2893003330 cites W2062050739 @default.
• W2893003330 cites W2063355986 @default.
• W2893003330 cites W2070230737 @default.
• W2893003330 cites W2083685172 @default.
• W2893003330 cites W2087070054 @default.
• W2893003330 cites W2091931191 @default.
• W2893003330 cites W2096457660 @default.
• W2893003330 cites W2103571364 @default.
• W2893003330 cites W2108005391 @default.
• W2893003330 cites W2108611680 @default.
• W2893003330 cites W2124669197 @default.
• W2893003330 cites W2126627603 @default.
• W2893003330 cites W2152376278 @default.
• W2893003330 cites W2157388483 @default.
• W2893003330 cites W2275792047 @default.
• W2893003330 cites W2411116122 @default.
• W2893003330 cites W2549907777 @default.
• W2893003330 cites W2582199324 @default.
• W2893003330 cites W2789942745 @default.
• W2893003330 cites W4211127808 @default.
• W2893003330 doi "https://doi.org/10.3233/hab-180350" @default.
• W2893003330 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30282350" @default.
• W2893003330 hasPublicationYear "2018" @default.
• W2893003330 type Work @default.
• W2893003330 sameAs 2893003330 @default.
• W2893003330 citedByCount "0" @default.
• W2893003330 crossrefType "journal-article" @default.
• W2893003330 hasAuthorship W2893003330A5016114675 @default.
• W2893003330 hasAuthorship W2893003330A5025147028 @default.
• W2893003330 hasAuthorship W2893003330A5034791601 @default.
• W2893003330 hasAuthorship W2893003330A5047400431 @default.
• W2893003330 hasAuthorship W2893003330A5083143645 @default.
• W2893003330 hasConcept C104317684 @default.
• W2893003330 hasConcept C153911025 @default.
• W2893003330 hasConcept C159654299 @default.
• W2893003330 hasConcept C185592680 @default.
• W2893003330 hasConcept C203014093 @default.
• W2893003330 hasConcept C2777991845 @default.
• W2893003330 hasConcept C2778502174 @default.
• W2893003330 hasConcept C28406088 @default.
• W2893003330 hasConcept C40767141 @default.
• W2893003330 hasConcept C54009773 @default.
• W2893003330 hasConcept C542903549 @default.
• W2893003330 hasConcept C54355233 @default.
• W2893003330 hasConcept C55493867 @default.
• W2893003330 hasConcept C81885089 @default.
• W2893003330 hasConcept C86803240 @default.
• W2893003330 hasConceptScore W2893003330C104317684 @default.
• W2893003330 hasConceptScore W2893003330C153911025 @default.
• W2893003330 hasConceptScore W2893003330C159654299 @default.
• W2893003330 hasConceptScore W2893003330C185592680 @default.
• W2893003330 hasConceptScore W2893003330C203014093 @default.
• W2893003330 hasConceptScore W2893003330C2777991845 @default.
• W2893003330 hasConceptScore W2893003330C2778502174 @default.
• W2893003330 hasConceptScore W2893003330C28406088 @default.
• W2893003330 hasConceptScore W2893003330C40767141 @default.
• W2893003330 hasConceptScore W2893003330C54009773 @default.
• W2893003330 hasConceptScore W2893003330C542903549 @default.
• W2893003330 hasConceptScore W2893003330C54355233 @default.
• W2893003330 hasConceptScore W2893003330C55493867 @default.
• W2893003330 hasConceptScore W2893003330C81885089 @default.
• W2893003330 hasConceptScore W2893003330C86803240 @default.
• W2893003330 hasLocation W28930033301 @default.
• W2893003330 hasLocation W28930033302 @default.
• W2893003330 hasOpenAccess W2893003330 @default.
• W2893003330 hasPrimaryLocation W28930033301 @default.
• W2893003330 hasRelatedWork W1590120095 @default.
• W2893003330 hasRelatedWork W1987801792 @default.
• W2893003330 hasRelatedWork W2009889818 @default.
• W2893003330 hasRelatedWork W2190999069 @default.
• W2893003330 hasRelatedWork W2311480185 @default.
• W2893003330 hasRelatedWork W2349557033 @default.
• W2893003330 hasRelatedWork W2380817849 @default.
• W2893003330 hasRelatedWork W2381276715 @default.

• next