FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893007297> ?p ?o ?g. }

• W2893007297 endingPage "58" @default.
• W2893007297 startingPage "47" @default.
• W2893007297 abstract "Extracellular Hsp70 (eHsp70) can activate immune cells via Toll-like receptors (TLR) 2 and 4, and induce cytokine synthesis. The aim of this study was to explore inflammation-associated effects of eHsp70 alone and in combination with cigarette smoke extract (CSE) in primary bronchial epithelial cells. We assessed IL-6 and IL-8 concentrations, TLR2, TLR4 and Hsp70 mRNA expressions, and mitogen-activated protein kinases (MAPKs) activation induced by recombinant human (rh) Hsp70, CSE or their combinations in normal human bronchial epithelial cells (NHBE) obtained commercially, and primary bronchial epithelial cells isolated from non-COPD lung donors (PBEC) or COPD patients (PBEC COPD). Baseline levels of IL-6 and IL-8 were significantly higher in PBEC COPD than in non-COPD PBECs. Upon rhHsp70 stimulation, IL-6 and IL-8 were significantly increased, with the strongest response in COPD-derived PBECs. CSE alone elevated cytokine secretion in all examined cells. rhHsp70 and CSE had antagonistic interactions on IL-8 release in PBECs from COPD patients, while the addition of rhHsp70 further increased CSE-induced IL-6 secretion in NHBE cells. rhHsp70 and CSE alone decreased TLR2 and TLR4 mRNA expression in COPD-derived PBECs. In non-COPD PBECs, combined treatments decreased only TLR2 mRNA expression. Hsp70 mRNA expression, as indicator of intracellular Hsp70, which may have anti-inflammatory effects, was reduced in COPD-derived cells upon exposure to CSE and rhHsp70 alone, but not with their combinations. Contrary to this, in PBECs from lung donors only combined treatments supressed Hsp70 gene expression. CSE activated JNK and p38 MAPKs, while rhHsp70 increased activation of c-Jun kinase in NHBE cells. Collectively, both eHsp70 and CSE induce pro-inflammatory responses in PBECs from non-COPD as well as COPD donors, but in combination antagonistic effects were observed in COPD-derived cells. These effects may be related to the regulation of TLR2/4 and might lead to modulation of inflammation with possible deleterious consequences for COPD patients." @default.
• W2893007297 created "2018-10-05" @default.
• W2893007297 creator A5002431071 @default.
• W2893007297 creator A5002953034 @default.
• W2893007297 creator A5005383444 @default.
• W2893007297 creator A5017952260 @default.
• W2893007297 creator A5029188076 @default.
• W2893007297 creator A5042282082 @default.
• W2893007297 date "2019-01-01" @default.
• W2893007297 modified "2023-10-12" @default.
• W2893007297 title "Pro-inflammatory effects of extracellular Hsp70 and cigarette smoke in primary airway epithelial cells from COPD patients" @default.
• W2893007297 cites W1515599953 @default.
• W2893007297 cites W1597335052 @default.
• W2893007297 cites W166076169 @default.
• W2893007297 cites W172217614 @default.
• W2893007297 cites W1969736341 @default.
• W2893007297 cites W1971423834 @default.
• W2893007297 cites W1989289468 @default.
• W2893007297 cites W1991765698 @default.
• W2893007297 cites W1992525781 @default.
• W2893007297 cites W1996486842 @default.
• W2893007297 cites W2003115478 @default.
• W2893007297 cites W2012382065 @default.
• W2893007297 cites W2020942835 @default.
• W2893007297 cites W2048497142 @default.
• W2893007297 cites W2055865155 @default.
• W2893007297 cites W2060729541 @default.
• W2893007297 cites W2083682196 @default.
• W2893007297 cites W2093779147 @default.
• W2893007297 cites W2094605832 @default.
• W2893007297 cites W2105260061 @default.
• W2893007297 cites W2113468396 @default.
• W2893007297 cites W2126102310 @default.
• W2893007297 cites W2128923889 @default.
• W2893007297 cites W2140133344 @default.
• W2893007297 cites W2141109742 @default.
• W2893007297 cites W2143112023 @default.
• W2893007297 cites W2149215904 @default.
• W2893007297 cites W2149477903 @default.
• W2893007297 cites W2150086428 @default.
• W2893007297 cites W2156309330 @default.
• W2893007297 cites W2159472629 @default.
• W2893007297 cites W2165410977 @default.
• W2893007297 cites W2235033695 @default.
• W2893007297 cites W224427771 @default.
• W2893007297 cites W2472929952 @default.
• W2893007297 cites W2515227717 @default.
• W2893007297 cites W2775144107 @default.
• W2893007297 cites W3150931801 @default.
• W2893007297 cites W4232534952 @default.
• W2893007297 cites W92308566 @default.
• W2893007297 doi "https://doi.org/10.1016/j.biochi.2018.09.010" @default.
• W2893007297 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30268700" @default.
• W2893007297 hasPublicationYear "2019" @default.
• W2893007297 type Work @default.
• W2893007297 sameAs 2893007297 @default.
• W2893007297 citedByCount "23" @default.
• W2893007297 countsByYear W28930072972019 @default.
• W2893007297 countsByYear W28930072972020 @default.
• W2893007297 countsByYear W28930072972021 @default.
• W2893007297 countsByYear W28930072972022 @default.
• W2893007297 countsByYear W28930072972023 @default.
• W2893007297 crossrefType "journal-article" @default.
• W2893007297 hasAuthorship W2893007297A5002431071 @default.
• W2893007297 hasAuthorship W2893007297A5002953034 @default.
• W2893007297 hasAuthorship W2893007297A5005383444 @default.
• W2893007297 hasAuthorship W2893007297A5017952260 @default.
• W2893007297 hasAuthorship W2893007297A5029188076 @default.
• W2893007297 hasAuthorship W2893007297A5042282082 @default.
• W2893007297 hasBestOaLocation W28930072972 @default.
• W2893007297 hasConcept C126322002 @default.
• W2893007297 hasConcept C170493617 @default.
• W2893007297 hasConcept C203014093 @default.
• W2893007297 hasConcept C2776070231 @default.
• W2893007297 hasConcept C2776780178 @default.
• W2893007297 hasConcept C2776914184 @default.
• W2893007297 hasConcept C2778690821 @default.
• W2893007297 hasConcept C2781236682 @default.
• W2893007297 hasConcept C68324877 @default.
• W2893007297 hasConcept C71924100 @default.
• W2893007297 hasConcept C86803240 @default.
• W2893007297 hasConceptScore W2893007297C126322002 @default.
• W2893007297 hasConceptScore W2893007297C170493617 @default.
• W2893007297 hasConceptScore W2893007297C203014093 @default.
• W2893007297 hasConceptScore W2893007297C2776070231 @default.
• W2893007297 hasConceptScore W2893007297C2776780178 @default.
• W2893007297 hasConceptScore W2893007297C2776914184 @default.
• W2893007297 hasConceptScore W2893007297C2778690821 @default.
• W2893007297 hasConceptScore W2893007297C2781236682 @default.
• W2893007297 hasConceptScore W2893007297C68324877 @default.
• W2893007297 hasConceptScore W2893007297C71924100 @default.
• W2893007297 hasConceptScore W2893007297C86803240 @default.
• W2893007297 hasFunder F4320322674 @default.
• W2893007297 hasLocation W28930072971 @default.
• W2893007297 hasLocation W28930072972 @default.
• W2893007297 hasLocation W28930072973 @default.
• W2893007297 hasLocation W28930072974 @default.
• W2893007297 hasOpenAccess W2893007297 @default.

• next