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- W2893025318 abstract "Abstract Macaque RFHV and LCV are close homologs of human KSHV and EBV, respectively. No experimental model of RFHV has been developed due to the lack of a source of culturable infectious virus. Screening of macaques at the Washington National Primate Research Center detected RFHV in saliva of SIV-infected macaques from previous vaccine studies. A pilot experimental infection of two naïve juvenile pig-tailed macaques was initiated by inoculation of saliva from SIV-infected pig-tailed and cynomolgus macaque donors, which contained high levels of DNA of the respective species-specific RFHV strain. Both juvenile recipients developed SIV and RFHV infections with RFHV DNA detected transiently in saliva and/or PBMC around week 16 post-infection. One juvenile macaque was infected with the homologous RFHVMn from whole saliva of a pig-tailed donor, which had been inoculated into the cheek pouch. This animal became immunosuppressed, developing simian AIDS and was euthanized 23 weeks after inoculation. The levels of RFHV DNA in saliva and PBMC remained below the level of detection after week 17, showing no reactivation of the RFHVMn infection during the rapid development of AIDS. The other juvenile macaque was infected with the heterologous RFHVMf from i.v. inoculation of purified virions from saliva of a cynomolgus donor. The juvenile recipient remained immunocompetent, developing high levels of persistent anti-RFHV and -SIV antibodies. After the initial presence of RFHVMf DNA in saliva and PBMC decreased to undetectable levels by week 19, all attempts to reactivate the infection through additional inoculations, experimental infection with purified SRV-2 or SIV, or immunosuppressive treatments with cyclosporine or dexamethasone were unsuccessful. An heterologous LCV transmission was also detected in this recipient, characterized by continual high levels of LCVMf DNA from the cynomolgus donor in both saliva and PBMC, coupled with high levels of anti-LCV antibodies. The macaque was sacrificed 209 weeks after the initial inoculation. Low levels of LCVMf DNA were detected in salivary glands, tonsils and other lymphoid organs, while RFHVMf DNA was below the level of detection. These results show successful co-transmission of RFHV and LCV from saliva and demonstrate differential lytic activation of the different gammaherpesvirus lineages due to presumed differences in biology and tropism and control by the host immune system. Although this initial pilot transmission study utilized only two macaques, it provides the first evidence for experimental transmission of the macaque homolog of KSHV, setting the stage for larger transmission studies to examine the differential activation of rhadinovirus and lymphocryptovirus infections and the pathological effects of immunosuppression." @default.
- W2893025318 created "2018-10-05" @default.
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- W2893025318 date "2018-10-01" @default.
- W2893025318 modified "2023-09-25" @default.
- W2893025318 title "Experimental co-transmission of simian immunodeficiency virus (SIV) and the macaque homologs of the Kaposi sarcoma-associated herpesvirus (KSHV) and Epstein-Barr virus (EBV)" @default.
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- W2893025318 cites W1574674240 @default.
- W2893025318 cites W1578954095 @default.
- W2893025318 cites W1579891230 @default.
- W2893025318 cites W1633682654 @default.
- W2893025318 cites W1964118024 @default.
- W2893025318 cites W1968817103 @default.
- W2893025318 cites W1977214514 @default.
- W2893025318 cites W1977303999 @default.
- W2893025318 cites W1977809195 @default.
- W2893025318 cites W1977937666 @default.
- W2893025318 cites W1984951112 @default.
- W2893025318 cites W1985102788 @default.
- W2893025318 cites W2002742326 @default.
- W2893025318 cites W2002880933 @default.
- W2893025318 cites W2005623684 @default.
- W2893025318 cites W2007028022 @default.
- W2893025318 cites W2007639993 @default.
- W2893025318 cites W2013980575 @default.
- W2893025318 cites W2014725234 @default.
- W2893025318 cites W2024864645 @default.
- W2893025318 cites W2025640003 @default.
- W2893025318 cites W2034399770 @default.
- W2893025318 cites W2036289720 @default.
- W2893025318 cites W2038502021 @default.
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- W2893025318 cites W2048997012 @default.
- W2893025318 cites W2052747456 @default.
- W2893025318 cites W2065884315 @default.
- W2893025318 cites W2067604812 @default.
- W2893025318 cites W2087025873 @default.
- W2893025318 cites W2090240281 @default.
- W2893025318 cites W2096651356 @default.
- W2893025318 cites W2096987374 @default.
- W2893025318 cites W2097716766 @default.
- W2893025318 cites W2098190927 @default.
- W2893025318 cites W2099188007 @default.
- W2893025318 cites W2102089980 @default.
- W2893025318 cites W2102274616 @default.
- W2893025318 cites W2104286263 @default.
- W2893025318 cites W2107149965 @default.
- W2893025318 cites W2109193316 @default.
- W2893025318 cites W2111253666 @default.
- W2893025318 cites W2111256536 @default.
- W2893025318 cites W2114519125 @default.
- W2893025318 cites W2117427088 @default.
- W2893025318 cites W2124248927 @default.
- W2893025318 cites W2125325745 @default.
- W2893025318 cites W2127039496 @default.
- W2893025318 cites W2127335488 @default.
- W2893025318 cites W2127798439 @default.
- W2893025318 cites W2130353041 @default.
- W2893025318 cites W2139567373 @default.
- W2893025318 cites W2141375573 @default.
- W2893025318 cites W2143493524 @default.
- W2893025318 cites W2144687970 @default.
- W2893025318 cites W2147428441 @default.
- W2893025318 cites W2147577279 @default.
- W2893025318 cites W2152717052 @default.
- W2893025318 cites W2155094763 @default.
- W2893025318 cites W2158986449 @default.
- W2893025318 cites W2168886958 @default.
- W2893025318 cites W2170096097 @default.
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- W2893025318 cites W2177264310 @default.
- W2893025318 cites W2275315944 @default.
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- W2893025318 doi "https://doi.org/10.1101/432351" @default.
- W2893025318 hasPublicationYear "2018" @default.
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