FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893050949> ?p ?o ?g. }

• W2893050949 endingPage "e1007629" @default.
• W2893050949 startingPage "e1007629" @default.
• W2893050949 abstract "Ammonia is a major signal that regulates nitrogen fixation in most diazotrophs. Regulation of nitrogen fixation by ammonia in the Gram-negative diazotrophs is well-characterized. In these bacteria, this regulation occurs mainly at the level of nif (nitrogen fixation) gene transcription, which requires a nif-specific activator, NifA. Although Gram-positive and diazotrophic Paenibacilli have been extensively used as a bacterial fertilizer in agriculture, how nitrogen fixation is regulated in response to nitrogen availability in these bacteria remains unclear. An indigenous GlnR and GlnR/TnrA-binding sites in the promoter region of the nif cluster are conserved in these strains, indicating the role of GlnR as a regulator of nitrogen fixation. In this study, we for the first time reveal that GlnR of Paenibacillus polymyxa WLY78 is essentially required for nif gene transcription under nitrogen limitation, whereas both GlnR and glutamine synthetase (GS) encoded by glnA within glnRA operon are required for repressing nif expression under excess nitrogen. Dimerization of GlnR is necessary for binding of GlnR to DNA. GlnR in P. polymyxa WLY78 exists in a mixture of dimers and monomers. The C-terminal region of GlnR monomer is an autoinhibitory domain that prevents GlnR from binding DNA. Two GlnR-biding sites flank the -35/-10 regions of the nif promoter of the nif operon (nifBHDKENXhesAnifV). The GlnR-binding site Ⅰ (located upstream of -35/-10 regions of the nif promoter) is specially required for activating nif transcription, while GlnR-binding siteⅡ (located downstream of -35/-10 regions of the nif promoter) is for repressing nif expression. Under nitrogen limitation, GlnR dimer binds to GlnR-binding siteⅠ in a weak and transient association way and then activates nif transcription. During excess nitrogen, glutamine binds to and feedback inhibits GS by forming the complex FBI-GS. The FBI-GS interacts with the C-terminal domain of GlnR and stabilizes the binding affinity of GlnR to GlnR-binding site Ⅱ and thus represses nif transcription." @default.
• W2893050949 created "2018-10-05" @default.
• W2893050949 creator A5005172217 @default.
• W2893050949 creator A5007950766 @default.
• W2893050949 creator A5013477568 @default.
• W2893050949 creator A5017797350 @default.
• W2893050949 creator A5022173400 @default.
• W2893050949 creator A5029265145 @default.
• W2893050949 creator A5032106989 @default.
• W2893050949 creator A5042462317 @default.
• W2893050949 creator A5044401948 @default.
• W2893050949 date "2018-09-28" @default.
• W2893050949 modified "2023-10-10" @default.
• W2893050949 title "Positive and negative regulation of transferred nif genes mediated by indigenous GlnR in Gram-positive Paenibacillus polymyxa" @default.
• W2893050949 cites W1497275823 @default.
• W2893050949 cites W1540254782 @default.
• W2893050949 cites W1586917242 @default.
• W2893050949 cites W1746223822 @default.
• W2893050949 cites W1804416415 @default.
• W2893050949 cites W1835434814 @default.
• W2893050949 cites W1842641861 @default.
• W2893050949 cites W1897119929 @default.
• W2893050949 cites W1978687530 @default.
• W2893050949 cites W1982830152 @default.
• W2893050949 cites W1983613885 @default.
• W2893050949 cites W1984469772 @default.
• W2893050949 cites W2016796204 @default.
• W2893050949 cites W2023798220 @default.
• W2893050949 cites W2024297040 @default.
• W2893050949 cites W2025812347 @default.
• W2893050949 cites W2028053857 @default.
• W2893050949 cites W2030164703 @default.
• W2893050949 cites W2030400863 @default.
• W2893050949 cites W2039833881 @default.
• W2893050949 cites W2054085503 @default.
• W2893050949 cites W2057257765 @default.
• W2893050949 cites W2060647962 @default.
• W2893050949 cites W2068150231 @default.
• W2893050949 cites W2076768215 @default.
• W2893050949 cites W2095570626 @default.
• W2893050949 cites W2104811944 @default.
• W2893050949 cites W2107082879 @default.
• W2893050949 cites W2107921073 @default.
• W2893050949 cites W2108811504 @default.
• W2893050949 cites W2109465920 @default.
• W2893050949 cites W2120674407 @default.
• W2893050949 cites W2125575209 @default.
• W2893050949 cites W2137397436 @default.
• W2893050949 cites W2137498721 @default.
• W2893050949 cites W2145483021 @default.
• W2893050949 cites W2157009395 @default.
• W2893050949 cites W2159156299 @default.
• W2893050949 cites W2161388525 @default.
• W2893050949 cites W2161773413 @default.
• W2893050949 cites W2164368007 @default.
• W2893050949 cites W2183867307 @default.
• W2893050949 cites W2289297095 @default.
• W2893050949 cites W2410775940 @default.
• W2893050949 cites W2418303045 @default.
• W2893050949 cites W2571766220 @default.
• W2893050949 cites W2747398312 @default.
• W2893050949 cites W4233997115 @default.
• W2893050949 doi "https://doi.org/10.1371/journal.pgen.1007629" @default.
• W2893050949 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6191146" @default.
• W2893050949 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30265664" @default.
• W2893050949 hasPublicationYear "2018" @default.
• W2893050949 type Work @default.
• W2893050949 sameAs 2893050949 @default.
• W2893050949 citedByCount "23" @default.
• W2893050949 countsByYear W28930509492019 @default.
• W2893050949 countsByYear W28930509492020 @default.
• W2893050949 countsByYear W28930509492021 @default.
• W2893050949 countsByYear W28930509492022 @default.
• W2893050949 countsByYear W28930509492023 @default.
• W2893050949 crossrefType "journal-article" @default.
• W2893050949 hasAuthorship W2893050949A5005172217 @default.
• W2893050949 hasAuthorship W2893050949A5007950766 @default.
• W2893050949 hasAuthorship W2893050949A5013477568 @default.
• W2893050949 hasAuthorship W2893050949A5017797350 @default.
• W2893050949 hasAuthorship W2893050949A5022173400 @default.
• W2893050949 hasAuthorship W2893050949A5029265145 @default.
• W2893050949 hasAuthorship W2893050949A5032106989 @default.
• W2893050949 hasAuthorship W2893050949A5042462317 @default.
• W2893050949 hasAuthorship W2893050949A5044401948 @default.
• W2893050949 hasBestOaLocation W28930509491 @default.
• W2893050949 hasConcept C101762097 @default.
• W2893050949 hasConcept C104317684 @default.
• W2893050949 hasConcept C138885662 @default.
• W2893050949 hasConcept C145244307 @default.
• W2893050949 hasConcept C150194340 @default.
• W2893050949 hasConcept C179926584 @default.
• W2893050949 hasConcept C181440489 @default.
• W2893050949 hasConcept C203075996 @default.
• W2893050949 hasConcept C2779134421 @default.
• W2893050949 hasConcept C2779289688 @default.
• W2893050949 hasConcept C2779349466 @default.
• W2893050949 hasConcept C41895202 @default.
• W2893050949 hasConcept C515207424 @default.

• next