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- W2893064518 abstract "Last year, Gerdes and colleagues published a paper (1) describing experiments that failed to support earlier work from their group (2, 3) which had implicated 10 type II toxin-antitoxin (TA) systems in the formation of antibiotic-tolerant Escherichia coli K-12 persister cells. The problem apparently arose as a result of contamination by and activation of the cryptic bacteriophage Φ80 in mutant strains lacking TA genes. A more recent paper by Goormaghtigh et al. (4) confirms and extends this reappraisal by providing evidence that an independently constructed E. coli K-12 mutant strain lacking the 10 type II TAs and free of phage contamination produced levels of persisters similar to those of wild-type bacteria after exposure to antibiotics (4). In addition, this work questions the validity of TA::green fluorescent protein (GFP) transcriptional reporter fusions (3). Since the possible link between TA systems and the persister phenotype is being studied in many laboratories, these corrections are both important and salutary." @default.
- W2893064518 created "2018-10-05" @default.
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- W2893064518 date "2018-11-07" @default.
- W2893064518 modified "2023-09-26" @default.
- W2893064518 title "Type II Toxin-Antitoxin Systems and Persister Cells" @default.
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- W2893064518 doi "https://doi.org/10.1128/mbio.01574-18" @default.
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