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• W2893082618 abstract "Microbial communities play an important role in organismal and ecosystem health. While high-throughput metabarcoding has revolutionized the study of bacterial communities, generating comparable viral communities has proven elusive, particularly in wildlife samples where the diversity of viruses and limited quantities of viral nucleic acid present distinctive challenges. Metagenomic sequencing is a promising solution for studying viral communities, but the lack of standardized methods currently precludes comparisons across host taxa or localities. Here, we developed an untargeted shotgun metagenomic sequencing protocol to generate comparable viral communities from noninvasively collected faecal and oropharyngeal swabs. Using samples from common vampire bats (Desmodus rotundus), a key species for virus transmission to humans and domestic animals, we tested how different storage media, nucleic acid extraction procedures and enrichment steps affect viral community detection. Based on finding viral contamination in foetal bovine serum, we recommend storing swabs in RNAlater or another nonbiological medium. We recommend extracting nucleic acid directly from swabs rather than from supernatant or pelleted material, which had undetectable levels of viral RNA. Results from a low-input RNA library preparation protocol suggest that ribosomal RNA depletion and light DNase treatment reduce host and bacterial nucleic acid, and improve virus detection. Finally, applying our approach to twelve pooled samples from seven localities in Peru, we showed that detected viral communities saturated at the attained sequencing depth, allowing unbiased comparisons of viral community composition. Future studies using the methods outlined here will elucidate the determinants of viral communities across host species, environments and time." @default.
• W2893082618 created "2018-10-05" @default.
• W2893082618 creator A5000112358 @default.
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• W2893082618 creator A5088259836 @default.
• W2893082618 date "2018-10-19" @default.
• W2893082618 modified "2023-10-03" @default.
• W2893082618 title "Using noninvasive metagenomics to characterize viral communities from wildlife" @default.
• W2893082618 cites W1552724685 @default.
• W2893082618 cites W1782790110 @default.
• W2893082618 cites W1948774627 @default.
• W2893082618 cites W1951724000 @default.
• W2893082618 cites W1957483571 @default.
• W2893082618 cites W1969236063 @default.
• W2893082618 cites W1969707722 @default.
• W2893082618 cites W1970067687 @default.
• W2893082618 cites W1970729332 @default.
• W2893082618 cites W1973324921 @default.
• W2893082618 cites W1974846245 @default.
• W2893082618 cites W1975175764 @default.
• W2893082618 cites W1977365703 @default.
• W2893082618 cites W1989889539 @default.
• W2893082618 cites W1992837491 @default.
• W2893082618 cites W1994421736 @default.
• W2893082618 cites W1997027215 @default.
• W2893082618 cites W2004280722 @default.
• W2893082618 cites W2005803754 @default.
• W2893082618 cites W2009906332 @default.
• W2893082618 cites W2013544175 @default.
• W2893082618 cites W2015035889 @default.
• W2893082618 cites W2022552842 @default.
• W2893082618 cites W2023517522 @default.
• W2893082618 cites W2025850587 @default.
• W2893082618 cites W2034215959 @default.
• W2893082618 cites W2038475803 @default.
• W2893082618 cites W2039583570 @default.
• W2893082618 cites W2040898355 @default.
• W2893082618 cites W2051356618 @default.
• W2893082618 cites W2059599514 @default.
• W2893082618 cites W2060036254 @default.
• W2893082618 cites W2066011810 @default.
• W2893082618 cites W2068945824 @default.
• W2893082618 cites W2073332363 @default.
• W2893082618 cites W2073911578 @default.
• W2893082618 cites W2073975144 @default.
• W2893082618 cites W2076037594 @default.
• W2893082618 cites W2078626331 @default.
• W2893082618 cites W2089212356 @default.
• W2893082618 cites W2095394401 @default.
• W2893082618 cites W2097146199 @default.
• W2893082618 cites W2107974372 @default.
• W2893082618 cites W2109947735 @default.
• W2893082618 cites W2110260027 @default.
• W2893082618 cites W2112874908 @default.
• W2893082618 cites W2113048642 @default.
• W2893082618 cites W2117106990 @default.
• W2893082618 cites W2119943561 @default.
• W2893082618 cites W2131171044 @default.
• W2893082618 cites W2131342905 @default.
• W2893082618 cites W2131851182 @default.
• W2893082618 cites W2134919433 @default.
• W2893082618 cites W2136114024 @default.
• W2893082618 cites W2139122528 @default.
• W2893082618 cites W2139702915 @default.
• W2893082618 cites W2141165258 @default.
• W2893082618 cites W2144340783 @default.
• W2893082618 cites W2144568881 @default.
• W2893082618 cites W2154760096 @default.
• W2893082618 cites W2156748860 @default.
• W2893082618 cites W2158998752 @default.
• W2893082618 cites W2159974882 @default.
• W2893082618 cites W2160762717 @default.
• W2893082618 cites W2161251845 @default.
• W2893082618 cites W2167391272 @default.
• W2893082618 cites W2167783407 @default.
• W2893082618 cites W2169945698 @default.
• W2893082618 cites W2170027074 @default.
• W2893082618 cites W2170486072 @default.
• W2893082618 cites W2172506017 @default.
• W2893082618 cites W2187687703 @default.
• W2893082618 cites W2215907530 @default.
• W2893082618 cites W2221549995 @default.
• W2893082618 cites W2233332950 @default.
• W2893082618 cites W2315497846 @default.
• W2893082618 cites W2340748026 @default.
• W2893082618 cites W2341347979 @default.
• W2893082618 cites W2376962651 @default.
• W2893082618 cites W2410580207 @default.
• W2893082618 cites W2465681871 @default.
• W2893082618 cites W2511649068 @default.
• W2893082618 cites W2523550005 @default.
• W2893082618 cites W2560765545 @default.
• W2893082618 cites W2567410363 @default.

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