FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893146965> ?p ?o ?g. }

• W2893146965 endingPage "12535" @default.
• W2893146965 startingPage "12527" @default.
• W2893146965 abstract "Pairing light and heavy chains in monoclonal antibodies (mAbs) using top-down (TD) or middle-down (MD) mass spectrometry (MS) may complement the sequence information on single chains provided by high-throughput genomic sequencing and bottom-up proteomics, favoring the rational selection of drug candidates. The 50 kDa F(ab) subunits of mAbs are the smallest structural units that contain the required information on chain pairing. These subunits can be enzymatically produced from whole mAbs and interrogated in their intact form by TD/MD MS approaches. However, the high structural complexity of F(ab) subunits requires increased sensitivity of the modern TD/MD MS for a comprehensive structural analysis. To address this and similar challenges, we developed and applied a multiplexed TD/MD MS workflow based on spectral averaging of tandem mass spectra (MS/MS) across multiple liquid chromatography (LC)–MS/MS runs acquired in reduced or full profile mode using an Orbitrap Fourier transform mass spectrometer (FTMS). We first benchmark the workflow using myoglobin as a reference protein, and then validate it for the analysis of the 50 kDa F(ab) subunit of a therapeutic mAb, trastuzumab. Obtained results confirm the envisioned benefits in terms of increased signal-to-noise ratio of product ions from utilizing multiple LC–MS/MS runs for TD/MD protein analysis using mass spectral averaging. The workflow performance is compared with the earlier introduced multiplexed TD/MD MS workflow based on transient averaging in Orbitrap FTMS. For the latter, we also report on enabling absorption mode FT processing and demonstrate its comparable performance to the enhanced FT (eFT) spectral representation." @default.
• W2893146965 created "2018-10-05" @default.
• W2893146965 creator A5002493964 @default.
• W2893146965 creator A5004595287 @default.
• W2893146965 creator A5022292935 @default.
• W2893146965 creator A5035195284 @default.
• W2893146965 creator A5039119223 @default.
• W2893146965 creator A5043588621 @default.
• W2893146965 creator A5068967401 @default.
• W2893146965 creator A5071352831 @default.
• W2893146965 creator A5073430364 @default.
• W2893146965 creator A5075723932 @default.
• W2893146965 creator A5079340352 @default.
• W2893146965 creator A5088326915 @default.
• W2893146965 date "2018-09-25" @default.
• W2893146965 modified "2023-10-17" @default.
• W2893146965 title "Multiplexed Middle-Down Mass Spectrometry as a Method for Revealing Light and Heavy Chain Connectivity in a Monoclonal Antibody" @default.
• W2893146965 cites W1964096679 @default.
• W2893146965 cites W1966227539 @default.
• W2893146965 cites W1970024552 @default.
• W2893146965 cites W1973495005 @default.
• W2893146965 cites W1983285663 @default.
• W2893146965 cites W1988396270 @default.
• W2893146965 cites W1991207270 @default.
• W2893146965 cites W2002222610 @default.
• W2893146965 cites W2005088298 @default.
• W2893146965 cites W2013365477 @default.
• W2893146965 cites W2019291685 @default.
• W2893146965 cites W2029325590 @default.
• W2893146965 cites W2040135401 @default.
• W2893146965 cites W2048461578 @default.
• W2893146965 cites W2048627194 @default.
• W2893146965 cites W2050139272 @default.
• W2893146965 cites W2050488655 @default.
• W2893146965 cites W2056580789 @default.
• W2893146965 cites W2059845531 @default.
• W2893146965 cites W2074186198 @default.
• W2893146965 cites W2078598194 @default.
• W2893146965 cites W2080139473 @default.
• W2893146965 cites W2090910993 @default.
• W2893146965 cites W2096488752 @default.
• W2893146965 cites W2132321369 @default.
• W2893146965 cites W2167056206 @default.
• W2893146965 cites W2175428996 @default.
• W2893146965 cites W2296811751 @default.
• W2893146965 cites W2418656493 @default.
• W2893146965 cites W2419323450 @default.
• W2893146965 cites W2557881040 @default.
• W2893146965 cites W2591798791 @default.
• W2893146965 cites W2604220201 @default.
• W2893146965 cites W2604431488 @default.
• W2893146965 cites W2607142718 @default.
• W2893146965 cites W2620867558 @default.
• W2893146965 cites W2750417386 @default.
• W2893146965 cites W2767144842 @default.
• W2893146965 cites W2769629480 @default.
• W2893146965 cites W2769766412 @default.
• W2893146965 cites W2777900905 @default.
• W2893146965 cites W2785258210 @default.
• W2893146965 cites W2786505886 @default.
• W2893146965 cites W2886934807 @default.
• W2893146965 cites W2949320474 @default.
• W2893146965 doi "https://doi.org/10.1021/acs.analchem.8b02398" @default.
• W2893146965 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30252447" @default.
• W2893146965 hasPublicationYear "2018" @default.
• W2893146965 type Work @default.
• W2893146965 sameAs 2893146965 @default.
• W2893146965 citedByCount "36" @default.
• W2893146965 countsByYear W28931469652018 @default.
• W2893146965 countsByYear W28931469652019 @default.
• W2893146965 countsByYear W28931469652020 @default.
• W2893146965 countsByYear W28931469652021 @default.
• W2893146965 countsByYear W28931469652022 @default.
• W2893146965 countsByYear W28931469652023 @default.
• W2893146965 crossrefType "journal-article" @default.
• W2893146965 hasAuthorship W2893146965A5002493964 @default.
• W2893146965 hasAuthorship W2893146965A5004595287 @default.
• W2893146965 hasAuthorship W2893146965A5022292935 @default.
• W2893146965 hasAuthorship W2893146965A5035195284 @default.
• W2893146965 hasAuthorship W2893146965A5039119223 @default.
• W2893146965 hasAuthorship W2893146965A5043588621 @default.
• W2893146965 hasAuthorship W2893146965A5068967401 @default.
• W2893146965 hasAuthorship W2893146965A5071352831 @default.
• W2893146965 hasAuthorship W2893146965A5073430364 @default.
• W2893146965 hasAuthorship W2893146965A5075723932 @default.
• W2893146965 hasAuthorship W2893146965A5079340352 @default.
• W2893146965 hasAuthorship W2893146965A5088326915 @default.
• W2893146965 hasConcept C113196181 @default.
• W2893146965 hasConcept C162356407 @default.
• W2893146965 hasConcept C185592680 @default.
• W2893146965 hasConcept C2777109674 @default.
• W2893146965 hasConcept C2780841973 @default.
• W2893146965 hasConcept C31827203 @default.
• W2893146965 hasConcept C43617362 @default.
• W2893146965 hasConceptScore W2893146965C113196181 @default.
• W2893146965 hasConceptScore W2893146965C162356407 @default.
• W2893146965 hasConceptScore W2893146965C185592680 @default.
• W2893146965 hasConceptScore W2893146965C2777109674 @default.

• next