Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893152168> ?p ?o ?g. }
- W2893152168 endingPage "2897" @default.
- W2893152168 startingPage "2887" @default.
- W2893152168 abstract "A major focus for our laboratory is identifying the molecules and mechanisms that regulate basolateral-to-apical transcytosis in polarized hepatocytes. Our most recent studies have focused on characterizing the biochemical and functional properties of the small rab17 GTPase. We determined that rab17 is a monosumoylated protein and that this modification likely mediates selective interactions with the apically located syntaxin 2. Using polarized hepatic WIF-B cells exogenously expressing wild-type, dominant active/guanosine triphosphate (GTP)-bound, dominant negative/guanosine diphosphate (GDP)-bound, or sumoylation-deficient/K68R rab17 proteins, we confirmed that rab17 regulates basolateral-to-apical transcytotic vesicle docking and fusion with the apical surface. We further confirmed that transcytosis is impaired from the subapical compartment to the apical surface and that GTP-bound and sumoylated rab17 are likely required for apical vesicle docking. Because expression of the GTP-bound rab17 led to impaired transcytosis, whereas wild type had no effect, we further propose that rab17 GTP hydrolysis is required for vesicle delivery. We also determined that transcytosis of three classes of newly synthesized apical residents showed similar responses to rab17 mutant expression, indicating that rab17 is a general component of the transcytotic machinery required for apically destined vesicle docking and fusion." @default.
- W2893152168 created "2018-10-05" @default.
- W2893152168 creator A5040325872 @default.
- W2893152168 creator A5049159802 @default.
- W2893152168 creator A5062329262 @default.
- W2893152168 creator A5062052747 @default.
- W2893152168 date "2018-11-15" @default.
- W2893152168 modified "2023-10-17" @default.
- W2893152168 title "Rab17 regulates apical delivery of hepatic transcytotic vesicles" @default.
- W2893152168 cites W1602063632 @default.
- W2893152168 cites W1671923285 @default.
- W2893152168 cites W1772190825 @default.
- W2893152168 cites W1778418746 @default.
- W2893152168 cites W1824067573 @default.
- W2893152168 cites W1843121983 @default.
- W2893152168 cites W1949551708 @default.
- W2893152168 cites W1966209618 @default.
- W2893152168 cites W1970977249 @default.
- W2893152168 cites W1973873018 @default.
- W2893152168 cites W1981345384 @default.
- W2893152168 cites W1984236884 @default.
- W2893152168 cites W1985509265 @default.
- W2893152168 cites W1990562688 @default.
- W2893152168 cites W1999748940 @default.
- W2893152168 cites W2000754635 @default.
- W2893152168 cites W2004839771 @default.
- W2893152168 cites W2007793507 @default.
- W2893152168 cites W2010936482 @default.
- W2893152168 cites W2011393081 @default.
- W2893152168 cites W2018289835 @default.
- W2893152168 cites W2019199442 @default.
- W2893152168 cites W2028590763 @default.
- W2893152168 cites W2029282032 @default.
- W2893152168 cites W2033483125 @default.
- W2893152168 cites W2039333617 @default.
- W2893152168 cites W2041640512 @default.
- W2893152168 cites W2046852407 @default.
- W2893152168 cites W2046873080 @default.
- W2893152168 cites W2054050573 @default.
- W2893152168 cites W2056164193 @default.
- W2893152168 cites W2056451594 @default.
- W2893152168 cites W2058148739 @default.
- W2893152168 cites W2058437595 @default.
- W2893152168 cites W2065103479 @default.
- W2893152168 cites W2065802258 @default.
- W2893152168 cites W2067914995 @default.
- W2893152168 cites W2070101581 @default.
- W2893152168 cites W2070996050 @default.
- W2893152168 cites W2074604248 @default.
- W2893152168 cites W2074782193 @default.
- W2893152168 cites W2074851426 @default.
- W2893152168 cites W2075568323 @default.
- W2893152168 cites W2079904892 @default.
- W2893152168 cites W2084959426 @default.
- W2893152168 cites W2090754575 @default.
- W2893152168 cites W2092236974 @default.
- W2893152168 cites W2100093548 @default.
- W2893152168 cites W2100837269 @default.
- W2893152168 cites W2103644870 @default.
- W2893152168 cites W2105827057 @default.
- W2893152168 cites W2108457537 @default.
- W2893152168 cites W2113189847 @default.
- W2893152168 cites W2128474008 @default.
- W2893152168 cites W2131649752 @default.
- W2893152168 cites W2133535035 @default.
- W2893152168 cites W2134484156 @default.
- W2893152168 cites W2134748481 @default.
- W2893152168 cites W2136193030 @default.
- W2893152168 cites W2142722979 @default.
- W2893152168 cites W2153259083 @default.
- W2893152168 cites W2156504663 @default.
- W2893152168 cites W2159332943 @default.
- W2893152168 cites W2164678697 @default.
- W2893152168 cites W2167638195 @default.
- W2893152168 cites W2293647937 @default.
- W2893152168 cites W2316574108 @default.
- W2893152168 cites W2406985299 @default.
- W2893152168 cites W2410826865 @default.
- W2893152168 cites W2416218655 @default.
- W2893152168 cites W263605040 @default.
- W2893152168 doi "https://doi.org/10.1091/mbc.e18-07-0433" @default.
- W2893152168 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6249867" @default.
- W2893152168 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30256711" @default.
- W2893152168 hasPublicationYear "2018" @default.
- W2893152168 type Work @default.
- W2893152168 sameAs 2893152168 @default.
- W2893152168 citedByCount "7" @default.
- W2893152168 countsByYear W28931521682019 @default.
- W2893152168 countsByYear W28931521682020 @default.
- W2893152168 countsByYear W28931521682021 @default.
- W2893152168 countsByYear W28931521682022 @default.
- W2893152168 crossrefType "journal-article" @default.
- W2893152168 hasAuthorship W2893152168A5040325872 @default.
- W2893152168 hasAuthorship W2893152168A5049159802 @default.
- W2893152168 hasAuthorship W2893152168A5062052747 @default.
- W2893152168 hasAuthorship W2893152168A5062329262 @default.
- W2893152168 hasBestOaLocation W28931521681 @default.
- W2893152168 hasConcept C118716 @default.