FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893203520> ?p ?o ?g. }

• W2893203520 abstract "About one-third of cellular proteins in eukaryotic cells are localized to membrane-enclosed organelles in the endomembrane system. Trafficking of these membrane proteins (including soluble lumenal proteins) among the organelles is mediated by small sac-like vesicles. Vesicle-mediated membrane trafficking regulates a broad range of biological processes, many of which are still poorly understood at the molecular level. A powerful approach to dissect a vesicle-mediated membrane trafficking pathway is unbiased genome-wide genetic screening, which only recently became possible in mammalian cells with the isolation of haploid human cell lines and the development of CRISPR-Cas9 genome editing. Here, we describe a FACS-based method to select populations of live mutant cells based on the surface levels of endogenous proteins or engineered reporters. Collection of these mutant populations enables subsequent deep sequencing and bioinformatics analysis to identify genes that regulate the trafficking pathway. This method can be readily adapted to genetically dissect a broad range of mammalian membrane trafficking processes using haploid genetics or CRISPR-Cas9 screens. © 2018 by John Wiley & Sons, Inc." @default.
• W2893203520 created "2018-10-05" @default.
• W2893203520 creator A5028789686 @default.
• W2893203520 creator A5028853083 @default.
• W2893203520 creator A5044270094 @default.
• W2893203520 creator A5048303779 @default.
• W2893203520 creator A5061532825 @default.
• W2893203520 creator A5070931753 @default.
• W2893203520 date "2018-09-28" @default.
• W2893203520 modified "2023-10-17" @default.
• W2893203520 title "Fluorescence Activated Cell Sorting (FACS) in Genome‐Wide Genetic Screening of Membrane Trafficking" @default.
• W2893203520 cites W1528367896 @default.
• W2893203520 cites W1934704664 @default.
• W2893203520 cites W1966446749 @default.
• W2893203520 cites W1973260880 @default.
• W2893203520 cites W1977709885 @default.
• W2893203520 cites W1982846895 @default.
• W2893203520 cites W1982873976 @default.
• W2893203520 cites W1990057935 @default.
• W2893203520 cites W1997372839 @default.
• W2893203520 cites W2003171404 @default.
• W2893203520 cites W2003787610 @default.
• W2893203520 cites W2009181620 @default.
• W2893203520 cites W2023527508 @default.
• W2893203520 cites W2024136850 @default.
• W2893203520 cites W2026592619 @default.
• W2893203520 cites W2031211461 @default.
• W2893203520 cites W2050480410 @default.
• W2893203520 cites W2064815984 @default.
• W2893203520 cites W2071004446 @default.
• W2893203520 cites W2110841199 @default.
• W2893203520 cites W2110951885 @default.
• W2893203520 cites W2131395804 @default.
• W2893203520 cites W2149491013 @default.
• W2893203520 cites W2152505144 @default.
• W2893203520 cites W2180291937 @default.
• W2893203520 cites W2426204902 @default.
• W2893203520 cites W2439020788 @default.
• W2893203520 cites W2511428910 @default.
• W2893203520 cites W2755239475 @default.
• W2893203520 cites W2911622295 @default.
• W2893203520 cites W4211197456 @default.
• W2893203520 cites W4233589827 @default.
• W2893203520 doi "https://doi.org/10.1002/cpcb.68" @default.
• W2893203520 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6386612" @default.
• W2893203520 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30265447" @default.
• W2893203520 hasPublicationYear "2018" @default.
• W2893203520 type Work @default.
• W2893203520 sameAs 2893203520 @default.
• W2893203520 citedByCount "6" @default.
• W2893203520 countsByYear W28932035202019 @default.
• W2893203520 countsByYear W28932035202020 @default.
• W2893203520 countsByYear W28932035202021 @default.
• W2893203520 countsByYear W28932035202023 @default.
• W2893203520 crossrefType "journal-article" @default.
• W2893203520 hasAuthorship W2893203520A5028789686 @default.
• W2893203520 hasAuthorship W2893203520A5028853083 @default.
• W2893203520 hasAuthorship W2893203520A5044270094 @default.
• W2893203520 hasAuthorship W2893203520A5048303779 @default.
• W2893203520 hasAuthorship W2893203520A5061532825 @default.
• W2893203520 hasAuthorship W2893203520A5070931753 @default.
• W2893203520 hasBestOaLocation W28932035201 @default.
• W2893203520 hasConcept C104317684 @default.
• W2893203520 hasConcept C119062480 @default.
• W2893203520 hasConcept C132455925 @default.
• W2893203520 hasConcept C141231307 @default.
• W2893203520 hasConcept C143065580 @default.
• W2893203520 hasConcept C144501496 @default.
• W2893203520 hasConcept C144647389 @default.
• W2893203520 hasConcept C1491633281 @default.
• W2893203520 hasConcept C158617107 @default.
• W2893203520 hasConcept C168240541 @default.
• W2893203520 hasConcept C206052861 @default.
• W2893203520 hasConcept C207177822 @default.
• W2893203520 hasConcept C2778630268 @default.
• W2893203520 hasConcept C41625074 @default.
• W2893203520 hasConcept C54355233 @default.
• W2893203520 hasConcept C559089031 @default.
• W2893203520 hasConcept C86803240 @default.
• W2893203520 hasConcept C95444343 @default.
• W2893203520 hasConcept C98108389 @default.
• W2893203520 hasConceptScore W2893203520C104317684 @default.
• W2893203520 hasConceptScore W2893203520C119062480 @default.
• W2893203520 hasConceptScore W2893203520C132455925 @default.
• W2893203520 hasConceptScore W2893203520C141231307 @default.
• W2893203520 hasConceptScore W2893203520C143065580 @default.
• W2893203520 hasConceptScore W2893203520C144501496 @default.
• W2893203520 hasConceptScore W2893203520C144647389 @default.
• W2893203520 hasConceptScore W2893203520C1491633281 @default.
• W2893203520 hasConceptScore W2893203520C158617107 @default.
• W2893203520 hasConceptScore W2893203520C168240541 @default.
• W2893203520 hasConceptScore W2893203520C206052861 @default.
• W2893203520 hasConceptScore W2893203520C207177822 @default.
• W2893203520 hasConceptScore W2893203520C2778630268 @default.
• W2893203520 hasConceptScore W2893203520C41625074 @default.
• W2893203520 hasConceptScore W2893203520C54355233 @default.
• W2893203520 hasConceptScore W2893203520C559089031 @default.
• W2893203520 hasConceptScore W2893203520C86803240 @default.
• W2893203520 hasConceptScore W2893203520C95444343 @default.
• W2893203520 hasConceptScore W2893203520C98108389 @default.

• next