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• W2893220144 endingPage "74" @default.
• W2893220144 startingPage "64" @default.
• W2893220144 abstract "We provide a kinetic characterization of (Na+, K+)-ATPase activity in a posterior gill microsomal fraction from a hololimnetic population of the diadromous Amazon River shrimp Macrobrachium amazonicum. Sucrose density gradient centrifugation reveals two distinct membrane fractions showing considerable (Na+, K+)ATP-ase activity, but also containing other microsomal ATPases. Only a single immune-reactive (Na+, K+)-ATPase with Mr of ≈110 kDa is present that hydrolyzes ATP with VM = 130.3 ± 4.8 nmol Pi min-1 mg protein-1 and K0.5 = 0.065 ± 0.00162 mmol L-1, exhibiting site-site interactions. Stimulation by Na+ (VM = 127.5 ± 5.3 nmol Pi min-1 mg protein-1, K0.5 = 5.3 ± 0.42 mmol L-1), Mg2+ (VM = 130.6 ± 6.8 nmol Pi min-1 mg protein-1, K0.5 = 0.33 ± 0.042 mmol L-1), K+ (VM = 126.7 ± 7.7 nmol Pi min-1 mg protein-1, K0.5 = 0.65 ± 0.0079 mmol L-1) and NH4+ (VM = 134.5 ± 8.6 nmol Pi min-1 mg protein-1, K0.5 = 1.28 ± 0.44 mmol L-1) also obeys cooperative kinetics. Ouabain (KI = 0.18 ± 0.058 mmol L-1) inhibits total ATPase activity by ≈70%. This study reveals considerable differences in the kinetic characteristics of the gill (Na+, K+)-ATPase in a hololimnetic population that appear to result from the adaptation of diadromous Macrobrachium amazonicum populations to different limnic habitats." @default.
• W2893220144 created "2018-10-05" @default.
• W2893220144 creator A5023304452 @default.
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• W2893220144 date "2019-01-01" @default.
• W2893220144 modified "2023-10-12" @default.
• W2893220144 title "Kinetic characterization of the gill (Na+, K+)-ATPase in a hololimnetic population of the diadromous Amazon River shrimp Macrobrachium amazonicum (Decapoda, Palaemonidae)" @default.
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• W2893220144 doi "https://doi.org/10.1016/j.cbpb.2018.09.004" @default.
• W2893220144 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30267892" @default.
• W2893220144 hasPublicationYear "2019" @default.
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