FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893227556> ?p ?o ?g. }

• W2893227556 abstract "Background. Norbormide (NRB) is a selective rat toxicant endowed with vasoconstrictor activity confined to the rat peripheral arteries. In a recent work we used a fluorescent derivative of NRB (NRB-AF12), obtained by coupling the NBD fluorophore to the parent molecule via a linker, in order to gain information about the possible site of action of the unlabelled compound. We found that NRB-AF12 labelled intracellular organelles in both NRB-sensitive and -insensitive cells and we accordingly proposed its use as a scaffold for the development of a new class of fluorescent probes. In this study, we examined the fluorescent properties of a BODIPY FL-conjugated NRB probe (MC009) developed: A) to verify if NRB distribution could be influenced by the attached fluorophore; B) to improve the fluorescent performance of NRB-AF12. Methods. MC009 characteristics were investigated by confocal fluorescence microscopy, in freshly isolated rat caudal artery myocytes (FIRCAM) and in LX2 cells, representative of NRB-sensitive and insensitive cells, respectively. Main results. In both FIRCAM and LX2 cells MC009 stained endoplasmic reticulum, mitochondria, Golgi apparatus and lipid droplets, revealing the same intracellular distribution as NRB-AF12, and, at the same time, had both improved photostability and gave a more intense fluorescent signal at lower concentrations than was possible with NRB-AF12, which resulted in a better and finer visualization of intracellular structures. Furthermore, MC009 was effective in cellular labelling in both living and fixed cells. At the concentration used to stain the cells, MC009 did not show any cytotoxic effect and did not affect the regular progression of cell cycle and division. Conclusions. This study demonstrates that the distribution of fluorescently labelled NRB is not affected by the type of fluorophore attached to the parent compound, supporting the idea that the localization of the fluorescent derivatives may reasonably reflect that of the parent compound. In addition, we observed a marked improvement in the fluorescent properties of BODIPY FL-conjugated NRB (MC009) over its NBD-derived counterpart (NRB-AF12), confirming NRB as a scaffold for the development of new, high performance, non-toxic fluorescent probes for the labelling of intracellular structures in both living and fixed cells." @default.
• W2893227556 created "2018-10-05" @default.
• W2893227556 creator A5014266275 @default.
• W2893227556 creator A5036706520 @default.
• W2893227556 creator A5043992105 @default.
• W2893227556 creator A5057012887 @default.
• W2893227556 creator A5069130616 @default.
• W2893227556 creator A5069875642 @default.
• W2893227556 creator A5072366209 @default.
• W2893227556 creator A5072785722 @default.
• W2893227556 creator A5088476604 @default.
• W2893227556 creator A5091398472 @default.
• W2893227556 date "2018-09-25" @default.
• W2893227556 modified "2023-10-02" @default.
• W2893227556 title "Synthesis and Biological Characterization of a New Norbormide Derived Bodipy FL-Conjugated Fluorescent Probe for Cell Imaging" @default.
• W2893227556 cites W1502026371 @default.
• W2893227556 cites W1923014952 @default.
• W2893227556 cites W1925922480 @default.
• W2893227556 cites W1975328187 @default.
• W2893227556 cites W1979220266 @default.
• W2893227556 cites W1985340929 @default.
• W2893227556 cites W1986164310 @default.
• W2893227556 cites W1986411648 @default.
• W2893227556 cites W2006872113 @default.
• W2893227556 cites W2009480581 @default.
• W2893227556 cites W2035068542 @default.
• W2893227556 cites W2038301088 @default.
• W2893227556 cites W2039879202 @default.
• W2893227556 cites W2045697933 @default.
• W2893227556 cites W2054431805 @default.
• W2893227556 cites W2057468776 @default.
• W2893227556 cites W2062334826 @default.
• W2893227556 cites W2077871784 @default.
• W2893227556 cites W2089960327 @default.
• W2893227556 cites W2092255988 @default.
• W2893227556 cites W2113981935 @default.
• W2893227556 cites W2114918609 @default.
• W2893227556 cites W2140411509 @default.
• W2893227556 cites W2236865793 @default.
• W2893227556 cites W2521227953 @default.
• W2893227556 cites W2588716287 @default.
• W2893227556 doi "https://doi.org/10.3389/fphar.2018.01055" @default.
• W2893227556 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6168047" @default.
• W2893227556 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30319407" @default.
• W2893227556 hasPublicationYear "2018" @default.
• W2893227556 type Work @default.
• W2893227556 sameAs 2893227556 @default.
• W2893227556 citedByCount "9" @default.
• W2893227556 countsByYear W28932275562019 @default.
• W2893227556 countsByYear W28932275562020 @default.
• W2893227556 countsByYear W28932275562021 @default.
• W2893227556 countsByYear W28932275562022 @default.
• W2893227556 crossrefType "journal-article" @default.
• W2893227556 hasAuthorship W2893227556A5014266275 @default.
• W2893227556 hasAuthorship W2893227556A5036706520 @default.
• W2893227556 hasAuthorship W2893227556A5043992105 @default.
• W2893227556 hasAuthorship W2893227556A5057012887 @default.
• W2893227556 hasAuthorship W2893227556A5069130616 @default.
• W2893227556 hasAuthorship W2893227556A5069875642 @default.
• W2893227556 hasAuthorship W2893227556A5072366209 @default.
• W2893227556 hasAuthorship W2893227556A5072785722 @default.
• W2893227556 hasAuthorship W2893227556A5088476604 @default.
• W2893227556 hasAuthorship W2893227556A5091398472 @default.
• W2893227556 hasBestOaLocation W28932275561 @default.
• W2893227556 hasConcept C111919701 @default.
• W2893227556 hasConcept C119062480 @default.
• W2893227556 hasConcept C121332964 @default.
• W2893227556 hasConcept C12554922 @default.
• W2893227556 hasConcept C158617107 @default.
• W2893227556 hasConcept C168240541 @default.
• W2893227556 hasConcept C169274487 @default.
• W2893227556 hasConcept C185592680 @default.
• W2893227556 hasConcept C2778617687 @default.
• W2893227556 hasConcept C2779178360 @default.
• W2893227556 hasConcept C2780557392 @default.
• W2893227556 hasConcept C41008148 @default.
• W2893227556 hasConcept C55493867 @default.
• W2893227556 hasConcept C62520636 @default.
• W2893227556 hasConcept C79879829 @default.
• W2893227556 hasConcept C86803240 @default.
• W2893227556 hasConcept C91881484 @default.
• W2893227556 hasConcept C95444343 @default.
• W2893227556 hasConceptScore W2893227556C111919701 @default.
• W2893227556 hasConceptScore W2893227556C119062480 @default.
• W2893227556 hasConceptScore W2893227556C121332964 @default.
• W2893227556 hasConceptScore W2893227556C12554922 @default.
• W2893227556 hasConceptScore W2893227556C158617107 @default.
• W2893227556 hasConceptScore W2893227556C168240541 @default.
• W2893227556 hasConceptScore W2893227556C169274487 @default.
• W2893227556 hasConceptScore W2893227556C185592680 @default.
• W2893227556 hasConceptScore W2893227556C2778617687 @default.
• W2893227556 hasConceptScore W2893227556C2779178360 @default.
• W2893227556 hasConceptScore W2893227556C2780557392 @default.
• W2893227556 hasConceptScore W2893227556C41008148 @default.
• W2893227556 hasConceptScore W2893227556C55493867 @default.
• W2893227556 hasConceptScore W2893227556C62520636 @default.
• W2893227556 hasConceptScore W2893227556C79879829 @default.
• W2893227556 hasConceptScore W2893227556C86803240 @default.
• W2893227556 hasConceptScore W2893227556C91881484 @default.
• W2893227556 hasConceptScore W2893227556C95444343 @default.

• next