FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893269170> ?p ?o ?g. }

• W2893269170 abstract "Abstract Disseminated infections with the fungal species Cryptococcus neoformans or, less frequently, C. gattii, are a leading cause of mortality in immunocompromised individuals. Central to the virulence of both species is an elaborate polysaccharide capsule that consists predominantly of glucuronoxylomannan (GXM). Due to its abundance, GXM is an ideal target for host antibodies, and several monoclonal antibodies (mAbs) have previously been derived using purified GXM or whole capsular preparations as antigen. In addition to their application in the diagnosis of cryptococcosis, anti-GXM mAbs are invaluable tools for studying capsule structure. In this study, we report the production and characterisation of a novel anti-GXM mAb, Crp127, that unexpectedly reveals a role for GXM remodelling during the process of fungal Titanisation. We show that Crp127 recognises a GXM epitope in an O -acetylation dependent, but xylosylation-independent, manner. The epitope is differentially expressed by the four main serotypes of Cryptococcus neoformans and gattii, is heterogeneously expressed within clonal populations of C. gattii serotype B strains and is typically confined to the central region of the enlarged capsule. Uniquely, however, this epitope redistributes to the capsular surface in Titan cells, a recently recognised subset of giant fungal cells that are produced in the host lung and are critical for successful infection. Crp127 therefore highlights hitherto unexpected features of cryptococcal morphological change and may hold significant therapeutic potential in differentially identifying cryptococcal strains and subtypes. Importance Cryptococcus neoformans and Cryptococcus gattii are the etiological agents of cryptococcosis, an invasive fungal infection responsible for approximately 200,000 deaths each year and 15% of AIDS-related deaths annually. Whilst the main virulence factor for both species is a highly variable polysaccharide capsule, formation of Titan cells also underlies the pathogenesis of C. neoformans. Previous studies have shown that capsule composition differs between yeast and Titan cells, however no clear distinctions in the expression or localisation of specific capsular epitopes have been made. In this study, we characterise a novel monoclonal antibody (mAb) specific to a capsular epitope that is differentially distributed throughout the capsules produced by yeast and Titan cells. Whilst this epitope is found within the midzone of yeast capsules, the presentation of this epitope on the surface of Titan cell capsules may represent a way in which these cell types are perceived differently by the immune system." @default.
• W2893269170 created "2018-10-05" @default.
• W2893269170 creator A5019397456 @default.
• W2893269170 creator A5023949391 @default.
• W2893269170 creator A5044430523 @default.
• W2893269170 creator A5058738414 @default.
• W2893269170 creator A5074728271 @default.
• W2893269170 creator A5075451807 @default.
• W2893269170 creator A5088886095 @default.
• W2893269170 date "2018-10-01" @default.
• W2893269170 modified "2023-09-23" @default.
• W2893269170 title "Redistribution of a glucuronoxylomannan epitope towards the capsule surface coincides with Titanisation in the human fungal pathogenCryptococcus neoformans" @default.
• W2893269170 cites W1040845057 @default.
• W2893269170 cites W1514158294 @default.
• W2893269170 cites W1539606662 @default.
• W2893269170 cites W1712993486 @default.
• W2893269170 cites W1845159538 @default.
• W2893269170 cites W1900943125 @default.
• W2893269170 cites W1934288202 @default.
• W2893269170 cites W1963311521 @default.
• W2893269170 cites W1963868317 @default.
• W2893269170 cites W1965580171 @default.
• W2893269170 cites W1972591128 @default.
• W2893269170 cites W1986415424 @default.
• W2893269170 cites W1996229802 @default.
• W2893269170 cites W1998820252 @default.
• W2893269170 cites W2000146338 @default.
• W2893269170 cites W2004173797 @default.
• W2893269170 cites W2005361432 @default.
• W2893269170 cites W2055416073 @default.
• W2893269170 cites W2063513729 @default.
• W2893269170 cites W2086188281 @default.
• W2893269170 cites W2089939918 @default.
• W2893269170 cites W2099573346 @default.
• W2893269170 cites W2103669668 @default.
• W2893269170 cites W2103699386 @default.
• W2893269170 cites W2112452073 @default.
• W2893269170 cites W2113306402 @default.
• W2893269170 cites W2115408128 @default.
• W2893269170 cites W2118218075 @default.
• W2893269170 cites W2124041958 @default.
• W2893269170 cites W2127552260 @default.
• W2893269170 cites W2132715320 @default.
• W2893269170 cites W2139842783 @default.
• W2893269170 cites W2140810025 @default.
• W2893269170 cites W2146256775 @default.
• W2893269170 cites W2150860035 @default.
• W2893269170 cites W2157311363 @default.
• W2893269170 cites W2159161460 @default.
• W2893269170 cites W2159733879 @default.
• W2893269170 cites W2162456739 @default.
• W2893269170 cites W2168471667 @default.
• W2893269170 cites W2168974130 @default.
• W2893269170 cites W2611697423 @default.
• W2893269170 cites W2755163126 @default.
• W2893269170 cites W2791041082 @default.
• W2893269170 cites W2791963883 @default.
• W2893269170 cites W2804701509 @default.
• W2893269170 cites W2892057274 @default.
• W2893269170 cites W2892176011 @default.
• W2893269170 cites W4245794307 @default.
• W2893269170 doi "https://doi.org/10.1101/431650" @default.
• W2893269170 hasPublicationYear "2018" @default.
• W2893269170 type Work @default.
• W2893269170 sameAs 2893269170 @default.
• W2893269170 citedByCount "0" @default.
• W2893269170 crossrefType "posted-content" @default.
• W2893269170 hasAuthorship W2893269170A5019397456 @default.
• W2893269170 hasAuthorship W2893269170A5023949391 @default.
• W2893269170 hasAuthorship W2893269170A5044430523 @default.
• W2893269170 hasAuthorship W2893269170A5058738414 @default.
• W2893269170 hasAuthorship W2893269170A5074728271 @default.
• W2893269170 hasAuthorship W2893269170A5075451807 @default.
• W2893269170 hasAuthorship W2893269170A5088886095 @default.
• W2893269170 hasBestOaLocation W28932691701 @default.
• W2893269170 hasConcept C10389963 @default.
• W2893269170 hasConcept C147483822 @default.
• W2893269170 hasConcept C159047783 @default.
• W2893269170 hasConcept C159654299 @default.
• W2893269170 hasConcept C195616568 @default.
• W2893269170 hasConcept C203014093 @default.
• W2893269170 hasConcept C2777893358 @default.
• W2893269170 hasConcept C2778952914 @default.
• W2893269170 hasConcept C2779286289 @default.
• W2893269170 hasConcept C2779413141 @default.
• W2893269170 hasConcept C542903549 @default.
• W2893269170 hasConcept C86803240 @default.
• W2893269170 hasConcept C89423630 @default.
• W2893269170 hasConceptScore W2893269170C10389963 @default.
• W2893269170 hasConceptScore W2893269170C147483822 @default.
• W2893269170 hasConceptScore W2893269170C159047783 @default.
• W2893269170 hasConceptScore W2893269170C159654299 @default.
• W2893269170 hasConceptScore W2893269170C195616568 @default.
• W2893269170 hasConceptScore W2893269170C203014093 @default.
• W2893269170 hasConceptScore W2893269170C2777893358 @default.
• W2893269170 hasConceptScore W2893269170C2778952914 @default.
• W2893269170 hasConceptScore W2893269170C2779286289 @default.
• W2893269170 hasConceptScore W2893269170C2779413141 @default.
• W2893269170 hasConceptScore W2893269170C542903549 @default.
• W2893269170 hasConceptScore W2893269170C86803240 @default.

• next