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- W2893299418 abstract "Intramolecular i-motifs of the form C3L3-8C3L3-8C3L3-8C3, where C3 denotes the cytosinestretch and L3-8 are “loop” regions containing any DNA base (L) including cytosine, were studied tounderstand the effects of loop length on i-motif stability. It contrast to the previously held notion thatlong-looped i-motifs are more stable, it was found that i-motif structures with short loops exhibithigher thermal stabilities and transitional pH values.The stability of long-looped i-motifs are then shown to increase with the addition of[Ru(phen)2dppz]2+ (phen = 1,10-phenanthroline, dppz = dipyrido [3,2-a:2',3'-c] phenazine); apolypyridyl complex that has a potential for photodynamic therapy. Addition of the complexenhances the stability of d(C3T838)3C3 but not that of d(C3T383)3C3, implying that loop lengths areimportant in defining i-motif-ligand interactions.The effects of loop base composition on the stability of i-motifs have also been presented. It isshown that when d(C3XYZ)3C3 sequences are used (where X and Z are adenine, thymine and guanine,and Y is any of the four DNA bases), pyrimidine-rich sequences form more stable i-motif structures.However, when guanine is X, only two out of 12 d(C3XYG)3C3 sequences were able to form i-motifs.Change in sequence direction also resulted in different thermal and pH stabilities; emphasising therole of loop base composition on not only the i-motif’s stability but also in its formation.X-ray crystallography was used to further understand the effects of loop bases on i-motifstructures. The study focuses on four tetramolecular i-motifs; two of which were solved in the mid1990’s;(d(C4)4 and d(C3T)4 but have now been re-examined using improved experimentalapproaches. Two novel i-motif structures of d(C3A)4 and [d(C3A) + d(C3T)] are presented.Following the X-ray diffraction of d(C3T)4 crystals to 0.68 Å resolution (previously reportedat 1.4 Å) at beamline I02 (Diamond Light Source Ltd.), a novel neutron diffraction study on theparticular i-motif was conducted. Single crystal neutron diffraction was carried out at MaNDibeamline (Spallation Neutron Source) to find the distribution of the proton between the hemiprotonatedcytosine+·cytosine base pairs and to understand the role that H-bonded water can play instabilising the i-motif structure." @default.
- W2893299418 created "2018-10-05" @default.
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- W2893299418 date "2018-01-01" @default.
- W2893299418 modified "2023-09-26" @default.
- W2893299418 title "Biophysical & crystallographic studies of DNA i-motifs" @default.
- W2893299418 hasPublicationYear "2018" @default.
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