Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893321395> ?p ?o ?g. }
- W2893321395 endingPage "230" @default.
- W2893321395 startingPage "223" @default.
- W2893321395 abstract "Angiotensin converting enzyme (ACE), fusing with FLAG tag, was overexpressed in human embryonic kidney 293T cells. This recombinant FLAG-tagged ACE was immobilized on anti-FLAG antibody coated magnetic beads by affinity method in crude cell lysate for the first time. The enzyme-immobilized magnetic beads (ACE-MB), without further cleavage procedure, were used directly to establish a cost-effective and reliable method for screening ACE inhibitors by coupling with fluorescence detection. The enzymatic activity of the ACE-MB was validated based on its Michaelian kinetic behavior towards hippuryl-histidyl-leucine by UHPLC-MS/MS method firstly. Then, several conditions were optimized including amount of magnetic beads, incubation temperature and time in the procedure of ACE immobilization and amount of ACE-MB in the microplate operation. Moreover, this screening assay was validated with Z′ factors between 0.71 and 0.81 using four known ACE inhibitors (captopril, lisinopril, fosinopril and fosinoprilat). The developed method was applied for the screening of ACE inhibitors from a small compound library of 45 natural products. As a result, epiberberine and fangchinoline with certain ACE inhibitory activities were screened out in the assay and validated. The results demonstrate the usefulness of this screening method using ACE immobilized on magnetic beads and the advantage of great efficiency with respect to both time and reagents for screening ACE inhibitors." @default.
- W2893321395 created "2018-10-05" @default.
- W2893321395 creator A5000168358 @default.
- W2893321395 creator A5008681979 @default.
- W2893321395 creator A5028858273 @default.
- W2893321395 creator A5035282947 @default.
- W2893321395 creator A5038667880 @default.
- W2893321395 creator A5060348081 @default.
- W2893321395 creator A5073070877 @default.
- W2893321395 creator A5091358989 @default.
- W2893321395 date "2019-02-01" @default.
- W2893321395 modified "2023-10-16" @default.
- W2893321395 title "A method using angiotensin converting enzyme immobilized on magnetic beads for inhibitor screening" @default.
- W2893321395 cites W1887255777 @default.
- W2893321395 cites W1965627867 @default.
- W2893321395 cites W1971303757 @default.
- W2893321395 cites W1971942980 @default.
- W2893321395 cites W1973348005 @default.
- W2893321395 cites W1980211728 @default.
- W2893321395 cites W1987145369 @default.
- W2893321395 cites W1987992329 @default.
- W2893321395 cites W1988771356 @default.
- W2893321395 cites W1990840149 @default.
- W2893321395 cites W1991001144 @default.
- W2893321395 cites W200018444 @default.
- W2893321395 cites W2000693703 @default.
- W2893321395 cites W2001290593 @default.
- W2893321395 cites W2006789324 @default.
- W2893321395 cites W2009682872 @default.
- W2893321395 cites W2017015661 @default.
- W2893321395 cites W2017284225 @default.
- W2893321395 cites W2020696766 @default.
- W2893321395 cites W2035671450 @default.
- W2893321395 cites W2059873767 @default.
- W2893321395 cites W2063688418 @default.
- W2893321395 cites W2079013207 @default.
- W2893321395 cites W2083457545 @default.
- W2893321395 cites W2093760059 @default.
- W2893321395 cites W2137080134 @default.
- W2893321395 cites W2165449806 @default.
- W2893321395 cites W2175228938 @default.
- W2893321395 cites W2217819442 @default.
- W2893321395 cites W2271194672 @default.
- W2893321395 cites W2335584316 @default.
- W2893321395 cites W2520199193 @default.
- W2893321395 cites W2528286804 @default.
- W2893321395 cites W2572330086 @default.
- W2893321395 cites W778019191 @default.
- W2893321395 doi "https://doi.org/10.1016/j.jpba.2018.09.054" @default.
- W2893321395 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30391811" @default.
- W2893321395 hasPublicationYear "2019" @default.
- W2893321395 type Work @default.
- W2893321395 sameAs 2893321395 @default.
- W2893321395 citedByCount "9" @default.
- W2893321395 countsByYear W28933213952019 @default.
- W2893321395 countsByYear W28933213952021 @default.
- W2893321395 countsByYear W28933213952022 @default.
- W2893321395 countsByYear W28933213952023 @default.
- W2893321395 crossrefType "journal-article" @default.
- W2893321395 hasAuthorship W2893321395A5000168358 @default.
- W2893321395 hasAuthorship W2893321395A5008681979 @default.
- W2893321395 hasAuthorship W2893321395A5028858273 @default.
- W2893321395 hasAuthorship W2893321395A5035282947 @default.
- W2893321395 hasAuthorship W2893321395A5038667880 @default.
- W2893321395 hasAuthorship W2893321395A5060348081 @default.
- W2893321395 hasAuthorship W2893321395A5073070877 @default.
- W2893321395 hasAuthorship W2893321395A5091358989 @default.
- W2893321395 hasConcept C104317684 @default.
- W2893321395 hasConcept C126838900 @default.
- W2893321395 hasConcept C181199279 @default.
- W2893321395 hasConcept C185592680 @default.
- W2893321395 hasConcept C27016395 @default.
- W2893321395 hasConcept C2776980637 @default.
- W2893321395 hasConcept C2777880890 @default.
- W2893321395 hasConcept C2779479957 @default.
- W2893321395 hasConcept C2779611605 @default.
- W2893321395 hasConcept C2780436477 @default.
- W2893321395 hasConcept C33633552 @default.
- W2893321395 hasConcept C40767141 @default.
- W2893321395 hasConcept C43617362 @default.
- W2893321395 hasConcept C51323132 @default.
- W2893321395 hasConcept C55493867 @default.
- W2893321395 hasConcept C71924100 @default.
- W2893321395 hasConcept C84393581 @default.
- W2893321395 hasConceptScore W2893321395C104317684 @default.
- W2893321395 hasConceptScore W2893321395C126838900 @default.
- W2893321395 hasConceptScore W2893321395C181199279 @default.
- W2893321395 hasConceptScore W2893321395C185592680 @default.
- W2893321395 hasConceptScore W2893321395C27016395 @default.
- W2893321395 hasConceptScore W2893321395C2776980637 @default.
- W2893321395 hasConceptScore W2893321395C2777880890 @default.
- W2893321395 hasConceptScore W2893321395C2779479957 @default.
- W2893321395 hasConceptScore W2893321395C2779611605 @default.
- W2893321395 hasConceptScore W2893321395C2780436477 @default.
- W2893321395 hasConceptScore W2893321395C33633552 @default.
- W2893321395 hasConceptScore W2893321395C40767141 @default.
- W2893321395 hasConceptScore W2893321395C43617362 @default.
- W2893321395 hasConceptScore W2893321395C51323132 @default.