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• W2893345441 abstract "Abstract The objective of our study was to determine the effect of 5% of CO 2 atmosphere and time of sample dilution on results of i n vitro analysis of stallion semen. Frozen-thawed semen from 14 stallions was incubated either in incubator or in a water bath, diluted prior to analysis or immediatelly after thawing. The following qualitative parameters were assessed after thawing (T0) and after 30 min (T30): motility in 3 sperm subpopulations (slow, medium, fast) defined by cluster analysis of parameters obtained by Computer Assisted Sperm Analysis, viability, acrosome and mitochondrial integrity. The slow subpopulation was only significantly reduced in diluted samples in CO 2 atmosphere at T0 (P < 0.05). In diluted samples the incubation time significantly affected distribution of fast, slow, and medium subpopulations in CO 2 and CO 2 free atmosphere (P < 0.05), respectively. Viability, acrosome and mitochondrial integrity were not affected by CO 2 atmosphere (P > 0.05), however acrosome (at T0) and mitochondrial integrity (at T30) were significantly higher in CO 2 atmosphere in non-diluted and diluted samples (P < 0.05), respectively. The results of the in vitro analysis of stallion semen were rather similar regardless of the atmosphere or dilution time." @default.
• W2893345441 created "2018-10-05" @default.
• W2893345441 creator A5062343395 @default.
• W2893345441 creator A5077790274 @default.
• W2893345441 date "2018-09-01" @default.
• W2893345441 modified "2023-09-26" @default.
• W2893345441 title "Effects of Various Incubation Conditions on Functional Parameters of Stallion Spermatozoa" @default.
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• W2893345441 doi "https://doi.org/10.2478/sab-2018-0026" @default.
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