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- W2893419887 abstract "Introduction The uterine myometrium is a unique immune environment, capable of harboring a ‘foreign’ fetus without eliciting an immune response. Objective In this pilot study we aimed to investigate the local presence, adaptation and function of human myometrial CD4 + T cells at the maternal-fetal interface in uncomplicated pregnancy. Methods Myometrial biopsies were obtained at caesarean section from placental and incision site. Lymphocytes were isolated through dissection and digestion with collagenase IV. CD4 + T cells were analyzed by flow cytometry or FACS sorted for RNA sequencing with CEL-seq protocol. Suppression assays were performed with FACS sorted uterine Tregs added to 15,000 healthy donor PBMC in different ratios, in 0.1 μg/mL anti-CD3 coated plates. Results Flow cytometry revealed that 70–80% of myometrial CD4 + T cells were CD69 + memory cells, suggesting a tissue-resident memory (TRM) phenotype. RNA sequencing confirmed a TRM-like profile in CD69 + cells, with high expression of CD49a, CXCR6, DUSP6, PD-1 and low expression of CD62L, KLF2/3 and S1PR1 compared to blood memory CD4 + T cells. Especially at the placental site, we observed a high expression of negative costimulatory molecules PD-1, TIGIT, Lag3, TIM-3 and CTLA4 and a high percentage of Tregs. Expression was intermediate at the incision site and low in blood. Suppression assays confirmed the suppressive capacity of myometrial Tregs. Conclusion Myometrial CD4 + T cells at the maternal-fetal interface are TRM cells with a high expression of negative costimulatory molecules and a high abundance of functional Tregs. The distribution pattern of these immunoregulatory features indicates that T cells may not only adapt to a tissue, but also to specific sites within one tissue, possibly depending on the local micro-environment." @default.
- W2893419887 created "2018-10-05" @default.
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- W2893419887 date "2018-10-01" @default.
- W2893419887 modified "2023-10-18" @default.
- W2893419887 title "243. Human myometrial T cells at the maternal-fetal interface are tissue-resident memory T cells, which show site-specific adaptation within one tissue" @default.
- W2893419887 doi "https://doi.org/10.1016/j.preghy.2018.08.102" @default.
- W2893419887 hasPublicationYear "2018" @default.
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