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• W2893424000 abstract "The objectives of this study were to evaluate the effects of collection day and 4-hour collection times on the urinary excretion of creatinine, purine derivatives (PD), and nitrogen compounds in grazing Zebu cattle. Five Nellore heifers (300 ± 15.2 kg body weight [BW]) were used in a 5 × 5 Latin square design. Five treatments were tested: a mineral mixture supplied at 60 g/day (g/d) (control – CON); two treatments higher in salt and urea to achieve a reduced ad libitum intake of supplements: one with 39.7% crude protein (CP) on a dry matter (DM) basis (RI40) and another with 69.4% CP (RI70), both fed ad libitum; and two supplements with restricted feeding rates relative to BW: both with 20.3% of CP fed at 5 g/kg BW (BW5) or 10 g/kg BW (BW10). Each experimental period lasted 17 d (12 used for adaptation and 5 for sample collection). Six collection times at 4-hour intervals were established for each 24-hour collection day. For total urine collection, a Foley catheter was used and the collection bag attached to the animal was emptied every 4 h. Collection day, collection time, treatment or their interactions (P > 0.05) had no effect on urinary excretions of creatinine, allantoin, uric acid, and PD, nor on their ratio with creatinine. However, an interaction between collection times and treatment (P < 0.05) was observed for the excretions of urinary nitrogen and urea nitrogen, and for their ratio with creatinine. The results supported the use of a single day-collection estimate the excretion of purine derivatives and nitrogen compounds in beef cattle kept in pasture. A single urine collection for 4-hour obtained at any time during the day was adequate to estimate the purine derivatives excretion. However, using a single 4-hour urine collection to estimate the urinary excretion of nitrogen compounds in grazing Zebu cattle is not recommended." @default.
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• W2893424000 date "2018-11-01" @default.
• W2893424000 modified "2023-10-14" @default.
• W2893424000 title "Evaluation of collection days and times to estimate urinary excretion of purine derivatives and nitrogen compounds in grazing Nellore cattle" @default.
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• W2893424000 doi "https://doi.org/10.1016/j.livsci.2018.09.016" @default.
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