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- W2893427075 abstract "Effect of long non-coding RNA AOC4P on gastrointestinal stromal tumor cells Jin-Chen Hu,1,* Quan Wang,2,* Li-Xin Jiang,1 Li Cai,3 Hui-Yuan Zhai,1 Zeng-Wu Yao,1 Meng-Lai Zhang,1 Ye Feng4 1Department of Gastrointestinal Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China; 2Department of Gastrointestinal and Anal Surgery, The First Hospital of Jilin University, Changchun, China; 3Department of Pathology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, China; 4Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital of Jilin University, Changchun, China *These authors contributed equally to this work Objective: In this research, we explored the effect of long non-coding RNA (lncRNA) AOC4P on gastrointestinal stromal tumor (GIST) cells. Materials and methods: The expression of lncRNA AOC4P in tissues was detected by real-time PCR (RT-PCR). The epithelial–mesenchymal transition (EMT)-related proteins in tissues were analyzed by Western blot. The experiment included negative control group (CN), silence AOC4P group (si AOC4P), and silence negative control group (si CT). RT-PCR, MTT, Scratch, Transwell, and Annexin V-FITC methods were used to detect the expression of lncRNA AOC4P, cell proliferation, cell migration ability, cell invasion ability, and apoptosis, respectively. The EMT-related proteins including TGF-β, ZEB1, Vimentin, Snail, and E-cadherin were analyzed by Western blot. Results: The expression of lncRNA AOC4P and the expression of EMT-related proteins in high-risk GISTs were higher than that in low- and intermediate-risk GISTs (P" @default.
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- W2893427075 date "2018-09-01" @default.
- W2893427075 modified "2023-09-22" @default.
- W2893427075 title "Effect of long non-coding RNA AOC4P on gastrointestinal stromal tumor cells" @default.
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- W2893427075 doi "https://doi.org/10.2147/ott.s174524" @default.
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