FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2893438498> ?p ?o ?g. }

• W2893438498 abstract "In Entamoeba histolytica, the EhADH adhesin together with the EhCP112 cysteine protease, form a 124 kDa complex named EhCPADH. This complex participates in trophozoite adherence, phagocytosis and cytolysis of target cells. EhCPADH and EhCP112 are both involved on epithelium damage, by opening tight junctions (TJ) and reaching other intercellular junctions. EhADH is a scaffold protein belonging to the ALIX family that contains a Bro1 domain, expresses at plasma membrane, endosomes and cytoplasm of trophozoites, and is also secreted to the medium. Contribution of EhADH to TJ opening still remains unknown. In this paper, to elucidate the role of EhADH on epithelium injury, we followed two strategies: producing a recombinant protein (rEhADH) and transfecting the ehadh gene in MDCK cells. Results from the first strategy revealed that rEhADH reached the intercellular space of epithelial cells and co-localized with claudin-1 and occludin at TJ region; later, rEhADH was mainly internalized by clathrin-coated vesicles. In the second strategy, MDCK cells expressing EhADH (MDCK-EhADH) showed the adhesin at plasma membrane. In addition, MDCK-EHADH cells exhibited adhesive features, producing epithelial aggregation and adherence to erythrocytes, as described in trophozoites. Surprisingly, the adhesin expression produced an increased amount of claudin-1, occludin, ZO-1 and ZO-2 at TJ, and also the transepithelial electric resistance (TEER), which is a measure of TJ gate function. Moreover, MDCK-EhADH cells resulted more susceptible to trophozoites attack, as showed by TEER and cytopathic experiments. Overall, our results indicated that EhADH affected TJ from the extracellular space and also intracellularly, suggesting that EhADH affects by itself TJ proteins, and possibly synergizes the action of other parasite molecules during epithelial invasion." @default.
• W2893438498 created "2018-10-05" @default.
• W2893438498 creator A5008340767 @default.
• W2893438498 creator A5051498950 @default.
• W2893438498 creator A5055280434 @default.
• W2893438498 creator A5069419406 @default.
• W2893438498 creator A5073394951 @default.
• W2893438498 creator A5087837931 @default.
• W2893438498 date "2018-09-28" @default.
• W2893438498 modified "2023-10-05" @default.
• W2893438498 title "Epithelial Cells Expressing EhADH, An Entamoeba histolytica Adhesin, Exhibit Increased Tight Junction Proteins" @default.
• W2893438498 cites W1533463539 @default.
• W2893438498 cites W1543548512 @default.
• W2893438498 cites W1602838328 @default.
• W2893438498 cites W1799705133 @default.
• W2893438498 cites W1827024784 @default.
• W2893438498 cites W1875628381 @default.
• W2893438498 cites W1970340893 @default.
• W2893438498 cites W1973466009 @default.
• W2893438498 cites W1978022020 @default.
• W2893438498 cites W1978397706 @default.
• W2893438498 cites W1985228626 @default.
• W2893438498 cites W1995980810 @default.
• W2893438498 cites W2000469457 @default.
• W2893438498 cites W2002066745 @default.
• W2893438498 cites W2020912749 @default.
• W2893438498 cites W2029171937 @default.
• W2893438498 cites W2040105120 @default.
• W2893438498 cites W2041454039 @default.
• W2893438498 cites W2048248702 @default.
• W2893438498 cites W2065100982 @default.
• W2893438498 cites W2073862596 @default.
• W2893438498 cites W2077752559 @default.
• W2893438498 cites W2083038106 @default.
• W2893438498 cites W2087814923 @default.
• W2893438498 cites W2096996795 @default.
• W2893438498 cites W2097046100 @default.
• W2893438498 cites W2097961311 @default.
• W2893438498 cites W2099646811 @default.
• W2893438498 cites W2100483442 @default.
• W2893438498 cites W2105167628 @default.
• W2893438498 cites W2112003767 @default.
• W2893438498 cites W2123712315 @default.
• W2893438498 cites W2133341114 @default.
• W2893438498 cites W2135325359 @default.
• W2893438498 cites W2151625646 @default.
• W2893438498 cites W2154203178 @default.
• W2893438498 cites W2155822490 @default.
• W2893438498 cites W2162351483 @default.
• W2893438498 cites W2166180305 @default.
• W2893438498 cites W2167879127 @default.
• W2893438498 cites W2168191922 @default.
• W2893438498 cites W2204093622 @default.
• W2893438498 cites W2286163175 @default.
• W2893438498 cites W2339338044 @default.
• W2893438498 cites W2363830885 @default.
• W2893438498 cites W2415591668 @default.
• W2893438498 cites W2472225832 @default.
• W2893438498 cites W2557523423 @default.
• W2893438498 cites W2570958552 @default.
• W2893438498 cites W2575368573 @default.
• W2893438498 cites W2729650180 @default.
• W2893438498 cites W2749668171 @default.
• W2893438498 cites W2766587854 @default.
• W2893438498 cites W2788820772 @default.
• W2893438498 cites W2808613196 @default.
• W2893438498 cites W4256321313 @default.
• W2893438498 cites W992193322 @default.
• W2893438498 doi "https://doi.org/10.3389/fcimb.2018.00340" @default.
• W2893438498 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6172307" @default.
• W2893438498 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30324093" @default.
• W2893438498 hasPublicationYear "2018" @default.
• W2893438498 type Work @default.
• W2893438498 sameAs 2893438498 @default.
• W2893438498 citedByCount "7" @default.
• W2893438498 countsByYear W28934384982019 @default.
• W2893438498 countsByYear W28934384982020 @default.
• W2893438498 countsByYear W28934384982022 @default.
• W2893438498 countsByYear W28934384982023 @default.
• W2893438498 crossrefType "journal-article" @default.
• W2893438498 hasAuthorship W2893438498A5008340767 @default.
• W2893438498 hasAuthorship W2893438498A5051498950 @default.
• W2893438498 hasAuthorship W2893438498A5055280434 @default.
• W2893438498 hasAuthorship W2893438498A5069419406 @default.
• W2893438498 hasAuthorship W2893438498A5073394951 @default.
• W2893438498 hasAuthorship W2893438498A5087837931 @default.
• W2893438498 hasBestOaLocation W28934384981 @default.
• W2893438498 hasConcept C104317684 @default.
• W2893438498 hasConcept C1491633281 @default.
• W2893438498 hasConcept C154317977 @default.
• W2893438498 hasConcept C177779419 @default.
• W2893438498 hasConcept C179470777 @default.
• W2893438498 hasConcept C190062978 @default.
• W2893438498 hasConcept C202751555 @default.
• W2893438498 hasConcept C2775962179 @default.
• W2893438498 hasConcept C2776867272 @default.
• W2893438498 hasConcept C29350253 @default.
• W2893438498 hasConcept C51872919 @default.
• W2893438498 hasConcept C529295009 @default.
• W2893438498 hasConcept C54355233 @default.

• next